anti-Zebrafish PDPK1 antibody for Western Blotting

Recommended PDPK1 Antibody (supplied by: Log in to see )

Antigen
3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) Antibodies
  • pdpk1
  • MGC82080
  • PDK1
  • PDPK2
  • PRO0461
  • Pdk1
  • zgc:153787
  • 1210
  • BG02759
  • CG1201
  • CG1210
  • DSTPK61
  • Dmel\\CG1210
  • Dstpk61
  • PDK
  • PDK-1
  • PDK1/Pk61C
  • PK61C
  • Pk61C
  • Pk61C/PDK1
  • dPDK-1
  • dPDK1
  • dSTPK61
  • pk61c
  • 3-phosphoinositide dependent protein kinase-1
  • 3-phosphoinositide dependent protein kinase 1
  • 3-phosphoinositide dependent protein kinase-1a
  • 3-phosphoinositide-dependent protein kinase 1
  • Phosphoinositide-dependent kinase 1
  • Protein PDK-1
  • PDPK1
  • pdpk1
  • Pdpk1
  • pdpk1a
  • Pdk1
  • pdk-1
Epitope
Middle Region
60
35
32
27
27
26
18
17
15
15
15
15
8
7
6
6
6
5
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish
465
220
220
19
13
11
10
10
6
4
3
2
2
1
1
1
1
1
1
1
Host
Rabbit
383
73
16
Clonality
Polyclonal
Conjugate
This PDPK1 antibody is un-conjugated
19
17
16
11
11
7
7
7
7
7
7
7
7
7
7
6
5
5
4
4
3
3
3
2
2
1
1
1
1
1
1
Application
Western Blotting (WB)
313
158
102
91
67
66
40
31
22
10
7
5
3
2
1
Options
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Catalog No. ABIN2779320
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN216809 WB Rabbit IgG AA 201-250 Log in to see Polyclonal
1 ABIN927288 WB Rabbit Middle Region Log in to see Polyclonal

General

Antigen 3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) Antibodies
Epitope Middle Region
(60), (35), (32), (27), (27), (26), (18), (17), (15), (15), (15), (15), (8), (7), (6), (6), (6), (5), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish
(465), (220), (220), (19), (13), (11), (10), (10), (6), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(383), (73), (16)
Clonality
Polyclonal
Conjugate This PDPK1 antibody is un-conjugated
(19), (17), (16), (11), (11), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (6), (5), (5), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(313), (158), (102), (91), (67), (66), (40), (31), (22), (10), (7), (5), (3), (2), (1)
Pubmed 1 reference available
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Product Details anti-PDPK1 Antibody

Target Details PDPK1 Application Details Handling ProductDetails: References for anti-PDPK1 antibody (ABIN2779320) Images
Blocking Peptide Blocking peptide for this product available: ABIN981552
Sequence IIHRDLKPEN ILLNEDMHIQ ITDFGTAKVL SPESKQARAN SFVGTAQYVS
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%
Characteristics This is a rabbit polyclonal antibody against PDPK1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human PDPK1

Target Details PDPK1

Product Details anti-PDPK1 Antibody Application Details Handling ProductDetails: References for anti-PDPK1 antibody (ABIN2779320) Images back to top
Antigen
Alternative Name PDPK1 (PDPK1 Antibody Abstract)
Background PDPK1 phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. It may play a general role in signaling processes and in development.
Alias Symbols: MGC20087, MGC35290, PDK1, PRO0461, PkB-like, PkB-like 1
Protein Interaction Partner: SRPK1, AKT1, APP, YWHAQ, STRAP, SGK1, UBC, SOCS3, PEA15, TCAP, ZNF133, SMARCB1, SBF1, XRCC6, BLMH, FAM213B, CARD11, C2orf44, KATNBL1, PNO1, DDIT4, PHAX, LUC7L2, ZC3HC1, GIT1, POLDIP2, XPO7, PRKCQ, G3BP1, PA2G4, SND1, TBL2, RPS6KB2, RPS6KB1, CDAN1, AKTIP,
Protein Size: 556
Molecular Weight 63 kDa
Gene ID 5170
NCBI Accession NM_002613, NP_002604
UniProt O15530
Research Area Kinases/Phosphatases
Pathways PI3K-Akt Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals

Application Details

Product Details anti-PDPK1 Antibody Target Details PDPK1 Handling ProductDetails: References for anti-PDPK1 antibody (ABIN2779320) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 556 AA

Restrictions For Research Use only

Handling

Product Details anti-PDPK1 Antibody Target Details PDPK1 Application Details ProductDetails: References for anti-PDPK1 antibody (ABIN2779320) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

ProductDetails: References for anti-PDPK1 antibody (ABIN2779320)

Product Details anti-PDPK1 Antibody Target Details PDPK1 Application Details Handling Images back to top
Background publications

Lin, Hsieh, Song, Lin: "Elevated phosphorylation and activation of PDK-1/AKT pathway in human breast cancer." in: British journal of cancer, Vol. 93, Issue 12, pp. 1372-81, 2005

Images

Product Details anti-PDPK1 Antibody Target Details PDPK1 Application Details Handling ProductDetails: References for anti-PDPK1 antibody (ABIN2779320) back to top
Supplier Images
Western Blotting (WB) image for anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (Middle Region) antibody (ABIN2779320) WB Suggested Anti-PDPK1 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:312500 Positiv...