anti-Human Tumor Necrosis Factor antibody for Flow Cytometry

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • TNF
  • tnf
  • dif
  • tnfa
  • xtnf
  • TNF-a
  • tnfsf2
  • tnf-alpha
  • TNF-alpha
  • DIF
  • TNFA
  • TNFSF2
  • TNFalpha
  • Tnfa
  • Tnfsf1a
  • RATTNF
  • TNFa
  • cTNF
  • TNF-ALPHA
  • Cachectin
  • Tnf-alpha
  • tnfa-like
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor
  • tumour necrosis factor alpha-like
  • tumor necrosis factor alpha
  • lipopolysaccharide-induced TNF factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • TNF
  • tnf
  • tnf-alpha
  • LITAF
  • Tnf
  • tnfb
Epitope
AA 77-233
51
43
29
27
24
22
17
17
15
11
11
10
10
8
4
4
4
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
1233
457
358
145
136
119
119
62
55
41
29
18
13
12
9
7
6
4
4
4
4
2
1
1
1
1
Host
Mouse
928
506
182
48
10
9
7
6
3
2
2
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
167
100
74
50
32
30
28
27
26
25
25
25
25
25
24
24
24
23
13
9
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Cell Culture (CC), ELISA, Flow Cytometry (FACS)
869
853
611
311
282
230
211
190
137
118
98
74
29
27
26
22
20
16
14
8
7
6
6
6
6
5
5
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN1997163
$ 308.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
14.354294 ABIN263070 ICC FACS IF IHC (p) FITC Mouse IgG1 kappa Log in to see MAb11 1
14.354294 ABIN263068 ELISA FACS ICC IF IHC IHC (fro) IHC (p) Mouse IgG1 kappa Log in to see MAb11 1
14.354294 ABIN1383957 FACS PE Mouse IgG1 Log in to see B-D9 1
14.354294 ABIN4898909 FACS Fluorescein Mouse IgG1 Log in to see 6402 4
14.354294 ABIN4898910 FACS PE Mouse IgG1 Log in to see 6402 7
14.354294 ABIN1383956 FACS FITC Mouse IgG1 Log in to see B-D9 1
14.354294 ABIN2662374 FACS Pacific Blue Mouse IgG1 kappa Log in to see MAb11 11
14.354294 ABIN2658839 FACS APC Mouse IgG1 kappa Log in to see MAb11 10
14.354294 ABIN2660503 FACS PerCP-Cy5.5 Mouse IgG1 kappa Log in to see MAb11 10
14.354294 ABIN2660627 FACS APC-Cy7 Mouse IgG1 kappa Log in to see MAb11 10
14.354294 ABIN2663940 FACS PE Mouse IgG1 kappa Log in to see MAb11 10
14.354294 ABIN2659787 BR FACS IF IHC PE/Dazzle™ 594 Mouse IgG1 kappa Log in to see MAb11 10
14.354294 ABIN2657587 FACS Alexa Fluor 488 Mouse IgG1 kappa Log in to see MAb11 10
14.354294 ABIN2657286 FACS Alexa Fluor 700 Mouse IgG1 kappa Log in to see MAb11 10
14.354294 ABIN2662036 FACS FITC Mouse IgG1 kappa Log in to see MAb11 11
14.354294 ABIN2659610 FACS PE-Cy7 Mouse IgG1 kappa Log in to see MAb11 10
14.354294 ABIN1997166 FACS FITC Mouse IgG1 AA 77-233 Log in to see 01
14.354294 ABIN1997168 FACS FITC Rabbit IgG AA 77-233 Log in to see 101
14.354294 ABIN5503748 FACS Fluorescein Mouse IgG1 AA 57-233, Membrane Form Log in to see 6401
14.354294 ABIN5503749 FACS Fluorescein Mouse IgG1 AA 57-233, Membrane Form Log in to see 6401

Top referenced anti-Tumor Necrosis Factor antibody for Flow Cytometry

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Application / Reactivity Human
Activity Inhibition (Act Inh) 2 Antibodies
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 13 Antibodies
Blocking Peptide (BP) 5 Antibodies
Blocking Reagent (BR) 5 Antibodies
Cell Culture (CC) 1 Antibodies
Cellular Assay (CA) 2 Antibodies
Cytometry by Time of Flight (CyTOF) 27 Antibodies
Dot Blot (DB) 2 Antibodies
ELISA 580 Antibodies
ELISA (Capture) 5 Antibodies
ELISA (Detection) 4 Antibodies
ELISpot 9 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 1 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 1 Antibodies
Enzyme Immunoassay (EIA) 49 Antibodies
Flow Cytometry (FACS) 509 Antibodies
Functional Studies (Func) 68 Antibodies
Immunoassay (IA) 13 Antibodies

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Epitope AA 77-233
(51), (43), (29), (27), (24), (22), (17), (17), (15), (11), (11), (10), (10), (8), (4), (4), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(1233), (457), (358), (145), (136), (119), (119), (62), (55), (41), (29), (18), (13), (12), (9), (7), (6), (4), (4), (4), (4), (2), (1), (1), (1), (1)
Host Mouse
(928), (506), (182), (48), (10), (9), (7), (6), (3), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(167), (100), (74), (50), (32), (30), (28), (27), (26), (25), (25), (25), (25), (25), (24), (24), (24), (23), (13), (9), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Cell Culture (CC), ELISA, Flow Cytometry (FACS)
(869), (853), (611), (311), (282), (230), (211), (190), (137), (118), (98), (74), (29), (27), (26), (22), (20), (16), (14), (8), (7), (6), (6), (6), (6), (5), (5), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling ProductDetails: References for anti-Tumor Necrosis Factor antibody (ABIN1997163) Images
Specificity Human TNFα
No cross-reactivity in WB and ELISA with
E.coli cell lysate
No Cross-Reactivity Escherichia coli (E. coli)
Characteristics This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Tumor necrosis factor-alpha (rhTNFα, ABIN2003122 Val 77-Leu 233, NP_000585.2,. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Purification purified by Protein A affinity chromatography
Sterility 0.2 μm filtered
Immunogen Recombinant Human TNFalpha protein (ABIN2003122)
Clone 9A7A5G9
Isotype IgG1

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling ProductDetails: References for anti-Tumor Necrosis Factor antibody (ABIN1997163) Images back to top
Antigen
Alternative Name TNF (TNF Antibody Abstract)
Background Tumor necrosis factor alpha (TNFα), also known as cachectin and TNFSF1A, is the prototypic cytokine of the the TNF superfamily. It is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNFα is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). Two receptors mediating the TNFα function have been identified, the ubiquitous TNF RI from which most signaling are derived, and the hematopoietic cell-restricted TNFRII. TNFα has been implicated in the pathogenesis of a variety of human diseases including autoimmune diseases, insulin resistance, and cancer.
NCBI Accession NP_000585.2
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling ProductDetails: References for anti-Tumor Necrosis Factor antibody (ABIN1997163) Images back to top
Application Notes ELISA: 0.5-1.0 μg/mL
This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect Human TNFα. The detection limit for Human TNFα is approximately 0.6 ng/well.
ELISA(Cap): 0.5-4 μg/mL
FCM: 0.5-2 μg/Test
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details ProductDetails: References for anti-Tumor Necrosis Factor antibody (ABIN1997163) Images back to top
Format Liquid
Buffer 0.2 μm filtered solution in PBS
Preservative Without preservative
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C,-80 °C
Storage Comment This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Expiry Date 12 months

ProductDetails: References for anti-Tumor Necrosis Factor antibody (ABIN1997163)

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Product cited in:

Cohen, Sabhachandani, Golberg, Konry: "Approaching near real-time biosensing: microfluidic microsphere based biosensor for real-time analyte detection." in: Biosensors & bioelectronics, Vol. 66, pp. 454-60, 2014

Images

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling ProductDetails: References for anti-Tumor Necrosis Factor antibody (ABIN1997163) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Tumor Necrosis Factor antibody (TNF) (AA 77-233) (ABIN1997163) Flow cytometric analysis of Purified anti-Human TNFa on CHO transfected cells and sti...