ISG15 Ubiquitin-Like Modifier (ISG15) (AA 2-157), (full length) protein (GST tag)

Details for Product No. ABIN1047870, Supplier: Log in to see
Protein Name
  • g1p2
  • zfISG15
  • si:rp71-1c23.2
  • ISG15
  • G1P2
  • IFI15
  • IP17
  • UCRP
  • hUCRP
  • G1p2
  • IGI15
  • Irfp
  • ISG15 ubiquitin-like modifier
  • isg15
  • ISG15
  • Isg15
Protein Characteristics
AA 2-157, full length
13
2
2
2
1
1
1
1
1
1
1
Origin
Human
23
10
1
Source
Escherichia coli (E. coli)
30
2
1
1
Protein Type
Recombinant
Purification tag / Conjugate
This ISG15 protein is labelled with GST tag.
Application
ELISA
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Sequence GWDLTVKMLA GNEFQVSLSS SMSVSELKAQ ITQKIGVHAF QQRLAVHPSG VALQDRVPLA SQGLGPGSTV LLVVDKCDEP LSILVRNNKG RSSTYEVRLT QTVAHLKQQV SGLEGVQDDL FWLTFEGKPL EDQLPLGEYG LKPLSTVFMN LRLRGG
Characteristics Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.
Purity 90 %
Background Ubiquitin-like protein that is conjugated to intracellular target proteins after IFN-alpha or IFN-beta stimulation. Its enzymatic pathway is partially distinct from that of ubiquitin, differing in substrate specificity and interaction with ligating enzymes. ISG15 conjugation pathway uses a dedicated E1 enzyme, but seems to converge with the Ub conjugation pathway at the level of a specific E2 enzyme. Targets include STAT1, SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, EIF2AK2/PKR, MX1/MxA, and RIG-1. Deconjugated by USP18/UBP43. Shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. May serve as a trans-acting binding factor directing the association of ligated target proteins to intermediate filaments. May also be involved in autocrine, paracrine and endocrine mechanisms, as in cell-to-cell signaling, possibly partly by inducing IFN-gamma secretion by monocytes and macrophages. Seems to display antiviral activity during viral infections. Ref.12 Ref.13 Ref.14 Ref.15 Ref.20 Ref.21 In response to IFN-tau secreted by the conceptus, may ligate to and regulate proteins involved in the release of prostaglandin F2-alpha (PGF), and thus prevent lysis of the corpus luteum and maintain the pregnancy By similarity. Ref.12 Ref.13 Ref.14 Ref.15 Ref.20 Ref.21
Molecular Weight 44.4 kD
UniProt Q64339
Comment

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

Restrictions For Research Use only
Format Lyophilized
Concentration 0.2-2 mg/mL
Buffer Tris-based buffer, 50 % glycerol
Handling Advice Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week
Storage -20 °C
Storage Comment Store at -20 °C for extended storage, conserve at -20 °C or -80 °C
Supplier Images
SDS-PAGE (SDS) image for ISG15 Ubiquitin-Like Modifier (ISG15) (AA 2-157), (full length) protein (GST tag) (ABIN1047870) ISG15 Ubiquitin-Like Modifier (ISG15) (AA 2-157), (full length) protein (GST tag)
Background publications Knight, Fahey, Cordova, Hillman, Kutny, Reich, Blomstrom: "A 15-kDa interferon-induced protein is derived by COOH-terminal processing of a 17-kDa precursor." in: The Journal of biological chemistry, Vol. 263, Issue 10, pp. 4520-2, 1988 (PubMed).

Reich, Evans, Levy, Fahey, Knight, Darnell: "Interferon-induced transcription of a gene encoding a 15-kDa protein depends on an upstream enhancer element." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 18, pp. 6394-8, 1987 (PubMed).

Blomstrom, Fahey, Kutny, Korant, Knight: "Molecular characterization of the interferon-induced 15-kDa protein. Molecular cloning and nucleotide and amino acid sequence." in: The Journal of biological chemistry, Vol. 261, Issue 19, pp. 8811-6, 1986 (PubMed).