D Amino Acid Oxidase Protein (AA 1-347) (His tag)
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- Target See all D Amino Acid Oxidase (DAO) Proteins
- D Amino Acid Oxidase (DAO) (D-Amino-Acid Oxidase (DAO))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-347
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This D Amino Acid Oxidase protein is labelled with His tag.
- Purpose
- Recombinant Human D-Amino-Acid Oxidase/DAO (N-6His)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MRVVVIGAGV IGLSTALCIH ERYHSVLQPL DIKVYADRFT PLTTTDVAAG LWQPYLSDPN NPQEADWSQQ TFDYLLSHVH SPNAENLGLF LISGYNLFHE AIPDPSWKDT VLGFRKLTPR ELDMFPDYGY GWFHTSLILE GKNYLQWLTE RLTERGVKFF QRKVESFEEV AREGADVIVN CTGVWAGALQ RDPLLQPGRG QIMKVDAPWM KHFILTHDPE RGIYNSPYII PGTQTVTLGG IFQLGNWSEL NNIQDHNTIW EGCCRLEPTL KNARIIGERT GFRPVRPQIR LEREQLRTGP SNTEVIHNYG HGGYGLTIHW GCALEAAKLF GRILEEKKLS RMPPSHL
- Characteristics
- Recombinant Human D-Amino-Acid Oxidase/DAO is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Leu347) of Human DAO fused with a 6His tag at the N-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product DAO Protein
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 150 mM NaCl, 1 mM DTT, 2 mM EDTA, pH 8.0 .
- Preservative
- Dithiothreitol (DTT)
- Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- D Amino Acid Oxidase (DAO) (D-Amino-Acid Oxidase (DAO))
- Alternative Name
- D-Amino-Acid Oxidase/DAO (DAO Products)
- Synonyms
- DAAO Protein, DAMOX Protein, OXDA Protein, daao Protein, oxda Protein, damox Protein, DAO Protein, Dao1 Protein, DKFZp469N2232 Protein, AI987963 Protein, Dao-1 Protein, SC5F2A.23c Protein, wu:fb55b11 Protein, zgc:113922 Protein, ABP Protein, ABP1 Protein, DAO1 Protein, KAO Protein, MGC114783 Protein, Abp Protein, Abp1 Protein, D-amino acid oxidase Protein, D-amino-acid oxidase Protein, D-amino-acid oxidase, tandem duplicate 1 Protein, amine oxidase, copper containing 1 Protein, D-amino-acid oxidase L homeolog Protein, DAO Protein, Dao Protein, dao Protein, SCO6740 Protein, dao.1 Protein, AOC1 Protein, PTRG_02224 Protein, SJAG_02496 Protein, dao.L Protein, Aoc1 Protein
- Sub Type
- Fusionprotein
- Background
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D-Amino-Acid Oxidase (DAO) belongs to the DAMOX/DASOX family. DAO is a peroxisomal enzyme which founctions as a homodimer to oxidizes D-amino acids to the corresponding imino acids, producing ammonia and hydrogen peroxide. D-amino-acid oxidase regulates the level of the neuromodulator D-serine in the brain, has a high activity towards D-DOPA and contributes to dopamine synthesis. D-amino-acid oxidase could act as a detoxifying agent which removes D-amino acids accumulated during aging. It also acts on a variety of D-amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups.
Alternative Names: D-Amino-Acid Oxidase, DAAO, DAMOX, DAO - Molecular Weight
- 41.64 kDa
- UniProt
- P14920
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