GNMT Protein (AA 2-294) (His tag)
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- Target See all GNMT Proteins
- GNMT (Glycine N-Methyltransferase (GNMT))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 2-294
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This GNMT protein is labelled with His tag.
- Purpose
- Recombinant Human Glycine N-Methyltransferase/GNMT (N-6His)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MVDSVYRTRS LGVAAEGLPD QYADGEAARV WQLYIGDTRS RTAEYKAWLL GLLRQHGCQR VLDVACGTGV DSIMLVEEGF SVTSVDASDK MLKYALKERW NRRHEPAFDK WVIEEANWMT LDKDVPQSAE GGFDAVICLG NSFAHLPDCK GDQSEHRLAL KNIASMVRAG GLLVIDHRNY DHILSTGCAP PGKNIYYKSD LTKDVTTSVL IVNNKAHMVT LDYTVQVPGA GQDGSPGLSK FRLSYYPHCL ASFTELLQAA FGGKCQHSVL GDFKPYKPGQ TYIPCYFIHV LKRTD
- Characteristics
- Recombinant Human Glycine N-Methyltransferase/GNMT is produced by our E. coli expression system. The target protein is expressed with sequence (Val2-Asg294) of Human GNMT fused with a 6His tag at the N-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product GNMT Protein
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Glycine N-Methyltransferase (GNMT) (AA 1-100) protein (GST tag)
GNMT Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Glycine N-Methyltransferase (GNMT) (AA 1-295) protein (His tag)GNMT Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS - PAGE SDS
Glycine N-Methyltransferase (GNMT) (AA 1-295) protein (GST tag)GNMT Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Glycine N-Methyltransferase (GNMT) (AA 2-295) protein (His tag)Crystallography grade GNMT Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Glycine N-Methyltransferase (GNMT) (AA 2-293) protein (His tag)Crystallography grade GNMT Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Glycine N-Methyltransferase (GNMT) (AA 1-295) (Active) protein (His tag)GNMT Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS - PAGE SDS Active
Glycine N-Methyltransferase (GNMT) protein (Myc-DYKDDDDK Tag)GNMT Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 150 mM NaCl, pH 8.0.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- GNMT (Glycine N-Methyltransferase (GNMT))
- Alternative Name
- Glycine-N-methyltransferase (GNMT Products)
- Synonyms
- glycine N-methyltransferase Protein, CpipJ_CPIJ015956 Protein, Gnmt Protein, GNMT Protein
- Sub Type
- Fusionprotein
- Background
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Glycine N-Methyltransferase (GNMT) is a tetrameric cytosolic protein. GNMT catalyzes the synthesis of N-methylglycine from glycine using S-adenosylmethionine (AdoMet) as the methyl donor. It can affects DNA methylation by regulating the ratio of S-adenosylmethionine to S-adenosylhomocystine, playing an important role in maintaining normal AdoMet levels. GNMT is highly expressed in liver. As a major folate-binding protein, GNMT takes part in the detoxification pathway. Defects in GNMT are the cause of hypermethioninemia. the patients with this deficiency are mild hepatomegaly and chronic elevation of serum transaminases.
Alternative Names: Glycine N-Methyltransferase, GNMT - Molecular Weight
- 34.9 kDa
- UniProt
- Q14749
- Pathways
- Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process
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