IL-6 Protein

Catalog No. ABIN1305110

Product Details

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Protein Type: Recombinant

Biological Activity: Active

Origin: Rat

Source: Escherichia coli (E. coli)

Application: ELISA, Functional Studies (Func)

Brand: BD Pharmingen™

Specificity: Biological Activity Range: Proliferation is measured using NFS-60 indicator cells
Specific Activity: 1.0 - 6.6 X 10^7 Units /mg
ED50: 0.15 - 1.0 ng/ml
Observed dose response: 60 fold A Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation. Since applications vary, titrate the reagent to obtain optimal results.

Characteristics: Recombinant rat IL-6 is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution, glycerol and 1.0 mg/ml biotechnology grade, low endotoxin bovine serum albumin, with no preservatives. The recombinant rat IL-6 was purified by immunoaffinity chromatography and was found to be > 95% pure by SDS-PAGE analysis, and an absorbance assay based on the Beers-Lambert law.

Purification: Immunoaffinity Chromatography
Recombinant rat IL-6 is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution, glycerol and bovine serum albumin, with no preservatives. Recombinant rat IL-6 is ≥ 95 % pure as determined by SDS-PAGE and an absorbance assay based on the Beers-Lambert law.

Purity: ≥ 95 %

Sterility: 0.22 μm filtered

Endotoxin Level: The endotoxin level is ≤ 0.1 ng per µg of rat IL-6, as measured in a chromogenic LAL assay.

Application Details

Application Notes: ELISA Standard: Recombinant rat IL-6 is useful as a quantitative standard for measuring rat IL-6 protein levels in a sandwich ELISA with the purified C3-4 antibody (Cat. No. 550644) as a capture antibody and the biotinylated G307-2 antibody (Cat. No. 550642) as the detection antibody. Note : This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assaying serum or plasma samples. For measuring rat IL-6 in serum or plasma our rat IL-6 OptEIA™ set (Cat. No. 550319) is specially formulated and recommended.

Comment:

BD Pharmingen™ Recombinant Rat IL-6 - Reactivity Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: Frozen aqueous buffered solution containing BSA and glycerol.

Preservative: Azide free

Handling Advice: Interleukin-6 (IL-6, aka IFN-β2, BSF-2, and HPGF) is a multifunctional cytokine which regulates immune responses, acute-phase reactions and hematopoiesis. Reported cellular targets for IL-6 action are hepatocytes, B and T lymphocytes, neurons, tumor cells and multi-potent hematopoietic cells.

Storage: -80 °C

Storage Comment: Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References

Van Snick: "Interleukin-6: an overview." in: Annual review of immunology, Vol. 8, pp. 253-78, (1990) (PubMed).

Chiu, Moulds, Coffman, Rennick, Lee: "Multiple biological activities are expressed by a mouse interleukin 6 cDNA clone isolated from bone marrow stromal cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, Issue 19, pp. 7099-103, (1988) (PubMed).

Van Snick, Cayphas, Szikora, Renauld, Van Roost, Boon, Simpson: "cDNA cloning of murine interleukin-HP1: homology with human interleukin 6." in: European journal of immunology, Vol. 18, Issue 2, pp. 193-7, (1988) (PubMed).

Van Snick, Cayphas, Vink, Uyttenhove, Coulie, Rubira, Simpson: "Purification and NH2-terminal amino acid sequence of a T-cell-derived lymphokine with growth factor activity for B-cell hybridomas." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 24, pp. 9679-83, (1987) (PubMed).

Weinstein, Ihle, Lavu, Reddy: "Truncation of the c-myb gene by a retroviral integration in an interleukin 3-dependent myeloid leukemia cell line." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 14, pp. 5010-4, (1986) (PubMed).

Target Details

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Alternative Name: IL-6 (IL6 Products)

Synonyms: BSF2 Protein, HGF Protein, HSF Protein, IFNB2 Protein, IL-6 Protein, Il-6 Protein, ILg6 Protein, Ifnb2 Protein, il6 Protein, CHIL-6 Protein, interleukin 6 Protein, interleukin-6 Protein, IL6 Protein, Il6 Protein, il-6 Protein, IL-6 Protein

Background: Interleukin-6 (IL-6, aka IFN-β2, BSF-2, and HPGF) is a multifunctional cytokine which regulates immune responses, acute-phase reactions and hematopoiesis. Reported cellular targets for IL-6 action are hepatocytes, B and T lymphocytes, neurons, tumor cells and multi-potent hematopoietic cells.

Pathways: TLR Signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome