IL-4 Protein

Catalog No. ABIN1305130

Product Details

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Protein Type: Recombinant

Origin: Rat

Source: Escherichia coli (E. coli)

Application: ELISA, Functional Studies (Func), Intracellular Flow Cytometry (ICFC), Blocking Reagent (BR)

Brand: BD Pharmingen™

Specificity: Biological Activity: This material has been shown to have biological activity. Carrier proteins should be pre-screened for possible effects in an appropriate experimental system. Carrier proteins may effect experimental results due to toxicity, high endotoxin levels or possible blocking activity.

Characteristics: Recombinant rat IL-4 is supplied as a frozen liquid comprised of 0.22 ìm sterile-filtered PBS, pH 7.2, and containing 2.0 mg/ml biotechnology grade, low endotoxin bovine serum albumin, with no preservatives. Recombinant rat IL-4 protein was purified by immunoaffinity chromatography and was found to be > 95% pure by SDS-PAGE analysis, and an absorbance assay based on the Beers-Lambert law.

Purification: Recombinant rat IL-4 protein was purified by immunoaffinity chromatography and was found to be > 95 % pure by SDS-PAGE analysis, and an absorbance assay based on the Beers-Lambert law.

Purity: ≥ 95 %

Sterility: 0.22 μm filtered

Endotoxin Level: The endotoxin level is ≤ 0.1 ng per µg of rat IL-4, as measured in a chromogenic LAL assay.

Application Details

Application Notes: ELISA Standard: Recombinant rat IL-4 (Cat. No. 555107) is useful as a quantitative standard for measuring rat IL-4 protein levels in an IL-4 specific sandwich ELISA with purified anti-rat IL-4, clone OX-81, (Cat. No. 555080) as a capture antibody and biotinylated anti-rat IL-4, clone B11-3 (Cat. No. 555090) as the detection antibody. To obtain linear standard curves, doubling dilutions of the rat IL-4 standard from ~2,000 to 15 pg/ml should be included in each ELISA plate.
Note: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assay of serum or plasma samples. For measuring rat IL-4 in serum or plasma our BD OptEIA™ Rat IL-4 ELISA Set (Cat. No. 555198) is specially formulated and recommended.

Comment:

BD Pharmingen™ Recombinant Rat IL-4

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant rat IL-4 is supplied as a frozen liquid comprised of 0.22 im sterile-filtered PBS, pH 7.2, and containing 2.0 mg/mL biotechnology grade, low endotoxin bovine serum albumin, with no preservatives.

Preservative: Azide free

Handling Advice: This preparation contains no preservatives, thus it should be handled under aseptic conditions. Rapidly thaw and quick-spin product prior to use. Recombinant rat IL-4 is supplied as a frozen liquid. Upon initial thawing the product should be aliquoted into polypropylene microtubes and frozen at -80°C for future use. Alternatively, the product can be diluted in sterile neutral buffer containing not less than 0.5 - 10 mg/ml carrier protein such as human or bovine albumin, aliquoted and stored at -80°C. For in vitro biological assay use, we recommend carrier-protein concentrations of 0.5 - 1.0 mg/ml. For use as an ELISA standard we recommend carrier-protein concentrations of 5 - 10 mg/ml.
Note: Failure to add carrier protein or store at indicated temperatures may result in a loss of activity.

Storage: -80 °C

Storage Comment: This preparation contains no preservatives, thus it should be handled under aseptic conditions. Rapidly thaw and quick-spin product prior to use. Store product at -80°C prior to use or for long term storage of stock solutions. Avoid multiple freeze-thaws of product. Recombinant rat IL-4 is supplied as a frozen liquid. Upon initial thawing the product should be aliquoted into polypropylene microtubes and frozen at -80°C for future use. Alternatively, the product can be diluted in sterile neutral buffer containing not less than 0.5 - 10 mg/ml carrier protein** such as human or bovine albumin, aliquoted and stored at -80°C. For in vitro biological assay use, we recommend carrier-protein concentrations of 0.5 - 1.0 mg/ml. For use as an ELISA standard we recommend carrier-protein concentrations of 5 - 10 mg/ml. NOTE: Failure to add carrier protein or store at indicated temperatures may result in a loss of activity.

References

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).

McKnight, Classon: "Biochemical and immunological properties of rat recombinant interleukin-2 and interleukin-4." in: Immunology, Vol. 75, Issue 2, pp. 286-92, (1992) (PubMed).

McKnight, Barclay, Mason: "Molecular cloning of rat interleukin 4 cDNA and analysis of the cytokine repertoire of subsets of CD4+ T cells." in: European journal of immunology, Vol. 21, Issue 5, pp. 1187-94, (1991) (PubMed).

Paul: "Interleukin-4: a prototypic immunoregulatory lymphokine." in: Blood, Vol. 77, Issue 9, pp. 1859-70, (1991) (PubMed).

Target Details

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Alternative Name: IL-4 (IL4 Products)

Synonyms: IL4 Protein, IL-4 Protein, BCGF-1 Protein, BCGF1 Protein, BSF-1 Protein, BSF1 Protein, Il-4 Protein, Il4e12 Protein, IL2 Protein, interleukin 4 Protein, IL4 Protein, Il4 Protein

Background: Interleukin-4 (IL-4) is a potent lymphoid cell growth factor which can stimulate the growth, differentiation and survivability of and regulate the function of various cell types of hematopoietic (including B and T lymphocytes, mast cells, monocytes and macrophages) and non-hematopoietic (e.g., vascular endothelial cells and certain tumor cells) origin. IL-4 exerts its biological activities by binding to specific high affinity receptors expressed by a wide variety of cell types. The rat IL-4 gene product is a 17 -20 kD glycoprotein containing 123 amino acid residues.

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation