FGFR1 Protein (Dimer, glycosylated, Soluble) (Fc Tag)

Catalog No. ABIN1589526

Product Details

Target: FGFR1 (Fibroblast Growth Factor Receptor 1 (FGFR1))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: glycosylated, Dimer, Soluble

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This FGFR1 protein is labelled with Fc Tag.

Sequence: RPSPTLPEQA QPWGAPVEVE SFLVHPGDLL QLRCRLRDDV QSINWLRDGV QLAESNRTRI TGEEVEVQDS VPADSGLYAC VTSSPSGSDT TYFSVNVSDA LPSSEDDDDD DDSSSEEKET DNTKPNRMPV APYWTSPEKM EKKLHAVPAA KTVKFKCPSS GTPNPTLRWL KNGKEFKPDH RIGGYKVRYA TWSIIMDSVV PSDKGNYTCI VENEYGSINH TYQLDVVERS PHRPILQAGL PANKTVALGS NVEFMCKVYS DPQPHIQWLK HIEVNGSKIG PDNLPYVQIL KTAGVNTTDK EMEVLHLRNV SFEDAGEYTC LAGNSIGLSH HSAWLTVLEA LEERPAVMTS PLYLEDPRRA SIEGRGDPEE PKSCDKTHTC PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRD ELTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG K

Characteristics: Length (AA): 601
Chromosomal location: 8p12
Determined by its ability to inhibit human FGF basic-dependent proliferation on HUVE cells

Purity: > 90 % by SDS-PAGE. Visualized by silver stain

Application Details

Comment:

Soluble Receptors

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: The lyophilized sFGFR-1/Fc is soluble in water and most aqueous buffers and should be reconstituted in PBS or medium to a concentration not lower than 50 μg/mL.

Buffer: PBS

Storage: -20 °C/-80 °C

Storage Comment: Lyophilized samples are stable for greater than six months at -20 °C to -70 °C. Reconstituted sFGFR-1/Fc should be stored in working aliquots at -20 °C.

Expiry Date: 6 months

References

Lohr, Mock, Beckhove, Herold-Mende: "Endothelial Cells Derived from Non-malignant Tissues Are of Limited Value as Models for Brain Tumor Vasculature." in: Anticancer research, Vol. 35, Issue 5, pp. 2681-90, (2015) (PubMed).

Kim, Al-Hilal, Chung, Kim, Ryu, Son, Byun: "Antiangiogenic and anticancer effect of an orally active low molecular weight heparin conjugates and its application to lung cancer chemoprevention." in: Journal of controlled release : official journal of the Controlled Release Society, Vol. 199, pp. 122-31, (2015) (PubMed).

Teodorczyk, Kleber, Wollny, Sefrin, Aykut, Mateos, Herhaus, Sancho-Martinez, Hill, Gieffers, Sykora, Weichert, Eisen, Trumpp, Sprick, Bergmann, Welsch, Martin-Villalba: "CD95 promotes metastatic spread via Sck in pancreatic ductal adenocarcinoma." in: Cell death and differentiation, Vol. 22, Issue 7, pp. 1192-202, (2015) (PubMed).

Target Details

Target: FGFR1 (Fibroblast Growth Factor Receptor 1 (FGFR1))

Alternative Name: FGFR-1 (FGFR1 Products)

Synonyms: FGFR Protein, fgfr Protein, X1FGFR Protein, XFGFR-1 Protein, XFGFRA2 Protein, bfgfr Protein, cek Protein, fgfr1 Protein, flg Protein, flt-2 Protein, flt2 Protein, kal2 Protein, ogd Protein, xfgfr1 Protein, CG7223 Protein, CT22273 Protein, CT39172 Protein, DFGF-R1 Protein, DFGF-R2 Protein, DFR-1 Protein, DFR1 Protein, DFR1/DFGF-R2 Protein, DPR3 Protein, DTRK(FR1) Protein, Dfr-1 Protein, Dfr1 Protein, DmHD-38 Protein, Dmel\\CG7223 Protein, Dtk1 Protein, EMS2 Protein, FGF-R2 Protein, FGFR2 Protein, FR1 Protein, Fr1 Protein, HD-38 Protein, HTL/FGFR1 Protein, Htl Protein, Tk1 Protein, dtk1 Protein, i100 Protein, i150 Protein, i79 Protein, j372 Protein, cd331 Protein, fgfbr Protein, fgfr-1 Protein, hbgfr Protein, n-sam Protein, ach Protein, cd333 Protein, cek2 Protein, fgfr-3 Protein, hsfgfr3ex Protein, jtk4 Protein, BFGFR Protein, CD331 Protein, CEK Protein, FGFBR Protein, FGFR-1 Protein, FLG Protein, FLT-2 Protein, FLT2 Protein, HBGFR Protein, HH2 Protein, KAL2 Protein, N-SAM Protein, OGD Protein, bFGF-R-1 Protein, AW208770 Protein, Eask Protein, Fgfr-1 Protein, Flt-2 Protein, Hspy Protein, FLG-1 Protein, FGFRI Protein, x1fgfr Protein, cb231 Protein, sb:cb231 Protein, fibroblast growth factor receptor Protein, fibroblast growth factor receptor 1 S homeolog Protein, basic fibroblast growth factor receptor 1 Protein, heartless Protein, fibroblast growth factor receptor 1 Protein, fibroblast growth factor receptor 3 (achondroplasia, thanatophoric dwarfism) L homeolog Protein, Fibroblast growth factor receptor 1 Protein, fibroblast growth factor receptor 1 L homeolog Protein, fibroblast growth factor receptor 1a Protein, FGFR Protein, fgfr Protein, fgfr1.S Protein, CpipJ_CPIJ005849 Protein, htl Protein, fgfr1 Protein, fgfr3.L Protein, FGFR1 Protein, Fgfr1 Protein, fgfr1.L Protein, fgfr1a Protein

Background: Recombinant human soluble FGFR-1 alpha (IIIc) was fused via a Xa cleavage site with the Fc part of human IgG1. Human recombinant soluble FGFR-1 alpha (IIIc)/Fc is a disulfide-linked heterodimeric protein. In the reduced form the glycosylated subunits of sFGFR-1 alpha/human Fc chimera display a molecular mass of 80-85 kDa. Fibroblast Growth Factors (FGFs) comprise a family of at least eighteen structurally related proteins that are involved in a multitude of physiological and pathological cellular processes, including cell growth, differentiation, angiogenesis, wound healing and tumorigenesis. The biological activities of the FGFs are mediated by a family of type I transmembrane tyrosine kinases which undergo dimerization and autophosphorylation after ligand binding. Four distinct genes encoding closely related FGF receptors, FGFR-1 to -4 are known. Multiple forms of FGFR-1 to -3 are generated by alternative splicing of the mRNAs. A frequent splicing event involving FGFR-1 and -2 results in receptors containing all three Ig domains, referred to as the alpha isoform, or only of the Ig-like domains IgII and IgIII, referred to as the ß isoform. Only the alpha isoform has been identified for FGFR-3 and FGFR-4. Additional splicing events for FGFR-1 to -3, involving the C-terminal half of the IgIII domain encoded by two mutually exclusive alternative exons, generate FGF receptors with alternative IgIII domains (IIIb and IIIc). A IIIa isoform which is a secreted FGF binding protein containing only the N-terminal half of the IgIII domain plus some intron sequences has also been reported for FGFR-1. Mutations in FGFR-1 to -3 have been found in patients with birth defects involving craniosynostosis.
Synonyms: FGFR1, CEK, FLG, OGD, FLT2, KAL2, BFGFR, CD331, FGFBR, FLT-2, HBGFR, N-SAM, FGFR-1, bFGF-R-1

Molecular Weight: 170.0 kDa

NCBI Accession: NM_023110, NP_075598

UniProt: P11362

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, S100 Proteins