N-Glycanase 1 Protein (NGLY1) (AA 1-333) (His tag)
-
- Target See all N-Glycanase 1 (NGLY1) Proteins
- N-Glycanase 1 (NGLY1)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-333
-
Origin
- Schizosaccharomyces pombe
-
Source
- Yeast
- Purification tag / Conjugate
- This N-Glycanase 1 protein is labelled with His tag.
- Application
- ELISA
- Sequence
- MDFHAISQRF IDMMRSKNSQ NASQPPETYP FYHEVRQMSQ HPWMYEDPEL QDYALSILPL DKLFQDASEL EKEGDGSWGY QDYVIQALLK WFKREFFVWV NQPPCEKCGG ETHMTGNGPP NEEEKWNGVR NVELYQCNVC GHNQRFPRYN RIRALLDSRK GRCGEWANCF TFLCRALGSR ARWIWNAEDH VWTEVYSNKQ QRWVHVDSGE ESFDEPLIYE QGWGKKMSYC LGFGIDSVRD VSHRYIRHPE NGLPRDRCPE SVLQQALHEI NIEFRSRLTD SERKALEEED KREKDELDGY MRPVSQATPT NTDLPARQTG NVEWKEKRGE AGK
- Specificity
- Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
- Characteristics
- Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.
- Purity
- > 90 %
- Top Product
- Discover our top product NGLY1 Protein
-
-
- Comment
-
The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Concentration
- 0.2-2 mg/mL
- Buffer
- Tris-based buffer, 50 % glycerol
- Handling Advice
- Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week
- Storage
- -20 °C
- Storage Comment
- Store at -20 °C, for extended storage, conserve at -20 °C or -80 °C.
-
- Target
- N-Glycanase 1 (NGLY1)
- Alternative Name
- Peptide-N (4)- (N-acetyl-beta-glucosaminyl)asparagine amidase (NGLY1 Products)
- Synonyms
- CDG1V Protein, PNG1 Protein, PNGase Protein, 1110002C09Rik Protein, Png1 Protein, im:6895151 Protein, zgc:110561 Protein, AtPNG1 Protein, K6M13.12 Protein, K6M13_12 Protein, peptide-N-glycanase 1 Protein, N-glycanase 1 Protein, N-glycanase 1 S homeolog Protein, peptide-N-glycanase 1 Protein, Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase Protein, similar to S. cerevisiae PNG1 (YPL096W) peptide:N-glycanase de-N-glycosylation enzyme Protein, NGLY1 Protein, ngly1.S Protein, ngly1 Protein, Ngly1 Protein, PNG1 Protein, png-1 Protein
- Background
-
Recommended name: Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase.
Short name= PNGase.
EC= 3.5.1.52.
Alternative name(s): Peptide:N-glycanase 1 - UniProt
- O74739
- Pathways
- Cell RedoxHomeostasis, SARS-CoV-2 Protein Interactome
-