Tumor Necrosis Factor (TNF) (AA 77-233) (Active) Protein

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Protein Name
  • TNF
  • tnf
  • dif
  • tnfa
  • xtnf
  • TNF-a
  • tnfsf2
  • tnf-alpha
  • TNF-alpha
  • DIF
  • TNFA
  • TNFSF2
  • TNFalpha
  • Tnfa
  • Tnfsf1a
  • RATTNF
  • TNFa
  • cTNF
  • TNF-ALPHA
  • Cachectin
  • Tnf-alpha
  • tnfa-like
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor
  • tumour necrosis factor alpha-like
  • tumor necrosis factor alpha
  • lipopolysaccharide-induced TNF factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • TNF
  • tnf
  • tnf-alpha
  • LITAF
  • Tnf
  • tnfb
Protein Characteristics
AA 77-233
29
20
12
11
7
5
5
5
4
4
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Origin
Human
122
51
30
15
11
8
7
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Source
HEK-293 Cells
167
29
15
4
4
3
3
2
2
2
2
1
1
Protein Type
Recombinant
Biological Activity
Active
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Brand ActiveMax®
Characteristics This protein carries no "tag". The protein has a calculated MW of 17.4 kDa. The protein migrates as 18 kDa under reducing (R) condition (SDS-PAGE).
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 0.1 EU per μg by the LAL method.
Grade HPLC verified
ProductDetails: Biological Activity Comment Biological Activity: The bio-activity was determined by its cytotoxcity assy using L929 Cells with 1ug/mL actinomycin D. ED50<25pg/mL, corresponding to a specific activity of >4X107 Unit/mg Gel Filtration analyisis of TNF alpha protein (ActiveMax®) and E.Coli derived TNF alpha protein

SDS-PAGE: The gel filtration analysis indicates that only HEK293 expressed TNF alpha protein is trimeric form , which has a molecular mass of 54 kDa.
Background Tumor necrosis factor alpha (TNFα) is a cytokine produced primarily by monocytes and macrophages. It is found in synovial cells and macrophages in the tissues.The primary role of TNFα is in the regulation of immune cells. TNFα is able to induce apoptotic cell death, to induce inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNFα production has been implicated in a variety of human diseases, including major depression, Alzheimer's disease and cancer. Recombinant TNFα is used as an immunostimulant under the INN tasonermin. TNFα can be produced ectopically in the setting of malignancy and parallels parathyroid hormone both in causing secondary hypercalcemia and in the cancers with which excessive production is associated.
Molecular Weight 17.4 kDa
NCBI Accession NP_000585
UniProt P01375
Research Area Inflammation, Cancer, Virology
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment Store at -20°C in lyophilized state after receipt. For long term storage, upon reconstitution rhTNFa should be aliquot and store at -20°C or -80°C.
Supplier Images
SDS-PAGE (SDS) image for Tumor Necrosis Factor (TNF) (AA 77-233) (Active) Protein (ABIN2181831) Human TNF-alpha (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel was...
Binding Studies (Bind) image for Tumor Necrosis Factor (TNF) (AA 77-233) (Active) Protein (ABIN2181831) Immobilized Human TNF-alpha can bind Humira with a linear range of 0.04-0.6 ng/mL.
Background publications Dowlati, Herrmann, Swardfager, Liu, Sham, Reim, Lanctôt: "A meta-analysis of cytokines in major depression." in: Biological psychiatry, Vol. 67, Issue 5, pp. 446-57, 2010 (PubMed).