FGF7 Protein
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- Target See all FGF7 Proteins
- FGF7 (Fibroblast Growth Factor 7 (FGF7))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- Western Blotting (WB)
- Characteristics
- The human FGF-7 lacking the signal sequence (1-31 aa) was expressed in E. coli and purified by the chromatographic procedures. This product is an intact enzyme without tag with 19 kDa size.
- Purity
- > 95 % as determined by SDS-PAGE (CBB staining)
- Sterility
- Sterile filtered
- Top Product
- Discover our top product FGF7 Protein
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- Application Notes
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1. Mitogen for epithelial cells
2. Western blot control for anti-FGF-7 antibodies
3. Acceleration of wound healing is implied.
4. Acceleration of hair development is implied. - Comment
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Activity: The ED50 as determined by a cell proliferation assay using MTS assay kit (CellTiter 96,Promega) with human keratinocyte JCRB141 cells was < 10 ng/mL.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
- PBS (10 mM Na-phosphate, 150 mM NaCl) pH 7.2, 50 % glycerol
- Handling Advice
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Avoid repeated freeze-thaw.
Quick freezing with liquid nitrogen and rapid thawing on water is desirable when necessary. - Storage
- -20 °C/-80 °C
- Storage Comment
- Store at -20 C (For long term storage: -80 C).
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Keratinocyte growth factor. A fibroblast growth factor family member with unusual target cell specificity." in: Annals of the New York Academy of Sciences, Vol. 638, pp. 62-77, (1992) (PubMed).
: "Purification and characterization of a newly identified growth factor specific for epithelial cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, Issue 3, pp. 802-6, (1989) (PubMed).
: "
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Keratinocyte growth factor. A fibroblast growth factor family member with unusual target cell specificity." in: Annals of the New York Academy of Sciences, Vol. 638, pp. 62-77, (1992) (PubMed).
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- Target
- FGF7 (Fibroblast Growth Factor 7 (FGF7))
- Alternative Name
- Keratinocyte Growth Factor (KGF/FGF7) (FGF7 Products)
- Synonyms
- HBGF-7 Protein, KGF Protein, Kgf Protein, kgf Protein, FGF-7 Protein, fgf7 Protein, fibroblast growth factor 7 Protein, fibroblast growth factor 7 L homeolog Protein, FGF7 Protein, Fgf7 Protein, fgf7 Protein, fgf7.L Protein
- Background
- Keratinocyte Growth Factor (KGF), also known as Fibroblast Growth Factor 7 (FGF-7), is a member of fibroblast growth factor (FGF) family. Although FGF-7 has heparin binding activity similar to FGF-1, its mitogenic activity is predominantly exhibited in keratinocytes. It is not effective to fibroblasts and endothelial cells.
- Gene ID
- 2252
- UniProt
- P21781
- Pathways
- RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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