TNFSF4 Protein (AA 51-183, N-Term)
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- Target See all TNFSF4 Proteins
- TNFSF4 (Tumor Necrosis Factor (Ligand) Superfamily, Member 4 (TNFSF4))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 51-183, N-Term
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Origin
- Human
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Source
- HEK-293 Cells
- Application
- Flow Cytometry (FACS), Immunohistochemistry (IHC)
- Purity
- > 95 % , as determined by Coomassie stained SDS-PAGE.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
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Less than 0.1 EU per μg of protein as determine by the LAL method.
- Top Product
- Discover our top product TNFSF4 Protein
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Biological activity: Human OX40L induces proliferation of PHA-activated human T cells in a dose dependent manner. The ED50 is 0.25 - 1.0 μg/mL. Results may vary depending on human donors and the dose of PHA.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
- For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.
- Buffer
- 0.22 μm filtered protein solution is in PBS.
- Handling Advice
- Avoid repeated freeze/thaw cycles.
- Storage
- -20 °C
- Storage Comment
- Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.
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- Target
- TNFSF4 (Tumor Necrosis Factor (Ligand) Superfamily, Member 4 (TNFSF4))
- Alternative Name
- OX40L (TNFSF4 Products)
- Synonyms
- TNFSF4 Protein, OX40L Protein, TNFRSF4 Protein, CD134L Protein, CD252 Protein, GP34 Protein, OX-40L Protein, OX4OL Protein, TXGP1 Protein, Ath-1 Protein, Ath1 Protein, Ox40l Protein, Txgp1l Protein, gp34 Protein, TNF superfamily member 4 Protein, tumor necrosis factor (ligand) superfamily, member 4 Protein, TNFSF4 Protein, Tnfsf4 Protein
- Background
- OX40L is a member of the TNF superfamily of structurally related proteins that exists primarily as a type II membrane bound, non-covalently linked homotrimeric protein. It is expressed on antigen presenting cells (APCs), such as dendritic cells and activated B cells. It is also expressed on vascular endothelial cells, mast cells, and natural killer cells. OX40L signals specifically through the OX40 receptor (CD134), which is expressed predominantly on CD4+ T cells, as well as on certain activated CD8+ T cells. OX40-OX40L interaction function as a costimulatory signal that is required for a conducive interaction between antigen presenting cells and their target T cells. OX40L enhances cellular proliferation and survival of memory and effector cells, and increases expression of RANTES, IL-2, IL-3, and IFN-γ. This interaction also induces immunoglobulin secretion by activated B cells. Besides playing a role in asthma, it has been shown that plasmacytoid dendritic cells (DCs) infiltrating a melanoma microenvironment are able to promote proinflammatory Th2 and regulatory immune profiles through OX40L and ICOSL expression. This suggests that this molecule could potentially be a therapeutic target for cancer treatment.
- Molecular Weight
- The 154 amino acid recombinant protein has a predicted molecular mass of approximately 17.6 kDa. The protein migrates at approximately 34 kDa in DTT-reducing conditions by SDS-PAGE. The predicted N-terminal amino acid is His.
- Pathways
- Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation, Cancer Immune Checkpoints
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