ALPL Protein (Transcript Variant 1) (His tag)
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- Target See all ALPL Proteins
- ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))
- Protein Type
- Recombinant
- Protein Characteristics
- Transcript Variant 1
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This ALPL protein is labelled with His tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human Alkaline phosphatase / ALPL (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- Endotoxin Level
- < 0.1 EU per μg protein as determined by LAL test
- Top Product
- Discover our top product ALPL Protein
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the N-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 20 mM HEPES, 150 mM NaCl, 2 mM MgSO4, 0.1 mM ZnCl2, pH 7.5. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))
- Alternative Name
- Alkaline Phosphatase,alpl (ALPL Products)
- Synonyms
- ALP Protein, iap Protein, alpl Protein, AP-TNAP Protein, APTNAP Protein, HOPS Protein, TNAP Protein, TNSALP Protein, Akp-2 Protein, Akp2 Protein, PHOA Protein, TNS-AP Protein, alkaline phosphatase, liver/bone/kidney L homeolog Protein, alkaline phosphatase, liver/bone/kidney Protein, alkaline phosphatase, intestinal, gene 2 Protein, alkaline phosphatase, liver/bone/kidney pseudogene Protein, alpl.L Protein, ALPL Protein, alpi.2 Protein, LOC100031702 Protein, Alpl Protein
- Background
- This gene encodes a member of the alkaline phosphatase family of proteins. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme may play a role in bone mineralization. Mutations in this gene have been linked to hypophosphatasia, a disorder that is characterized by hypercalcemia and skeletal defects.
- Molecular Weight
- 54.5 kDa
- NCBI Accession
- NP_000469
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