PCBP2 Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)
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- Target See all PCBP2 Proteins
- PCBP2 (Poly(rC) Binding Protein 2 (PCBP2))
- Protein Type
- Recombinant
- Protein Characteristics
- Transcript Variant 2
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This PCBP2 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human PCBP2 / hnRNP-E2 (transcript variant 2) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product PCBP2 Protein
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- PCBP2 (Poly(rC) Binding Protein 2 (PCBP2))
- Alternative Name
- Pcbp2,hnrnp-e2 (PCBP2 Products)
- Synonyms
- HNRNPE2 Protein, HNRPE2 Protein, hnRNP-E2 Protein, AW412548 Protein, Hnrpx Protein, alphaCP-2 Protein, PCBP3 Protein, fb36h02 Protein, wu:fb36h02 Protein, zgc:55964 Protein, PCBP2 Protein, poly(rC) binding protein 2 Protein, poly(rC) binding protein 2 L homeolog Protein, PCBP2 Protein, Pcbp2 Protein, pcbp2 Protein, pcbp2.L Protein
- Background
- The protein encoded by this gene appears to be multifunctional. Along with PCBP-1 and hnRNPK, it is one of the major cellular poly(rC)-binding proteins. The encoded protein contains three K-homologous (KH) domains which may be involved in RNA binding. Together with PCBP-1, this protein also functions as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This multiexon structural mRNA is thought to be retrotransposed to generate PCBP-1, an intronless gene with functions similar to that of PCBP2. This gene and PCBP-1 have paralogous genes (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. Thsi gene also has two processed pseudogenes (PCBP2P1 and PCBP2P2). Multiple transcript variants encoding different isoforms have been found for this gene.
- Molecular Weight
- 38 kDa
- NCBI Accession
- NP_114366
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