Dihydrofolate Reductase Protein (DHFR) (AA 1-187) (His tag)

Catalog No. ABIN667350

Product Details

Target: Dihydrofolate Reductase (DHFR)

Protein Type: Recombinant

Protein Characteristics: AA 1-187

Origin: Mouse

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This Dihydrofolate Reductase protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Characteristics: DHFR, 1-187aa, Mouse, His tag, E.coli

Purity: > 95 % by SDS - PAGE

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/ml (determined by Bradford assay)

Buffer: Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.

Storage: 4 °C

Target Details

Target: Dihydrofolate Reductase (DHFR)

Alternative Name: DHFR (DHFR Products)

Synonyms: DHFR Protein, F10_orf160 Protein, ECK0049 Protein, JW0047 Protein, tmrA Protein, cb595 Protein, zgc:86637 Protein, DDBDRAFT_0187132 Protein, DDBDRAFT_0230113 Protein, DDB_0187132 Protein, DDB_0230113 Protein, CG14887 Protein, Dmel\\CG14887 Protein, dhfr Protein, DHFRP1 Protein, DYR Protein, 8430436I03Rik Protein, AA607882 Protein, AI662710 Protein, AW555094 Protein, Dhfr1 Protein, confers resistance to sulfonamides Protein, dihydrofolate reductase Protein, dihydrofolate reductase DhfR Protein, Dihydrofolate reductase Protein, dhfr Protein, DHFR Protein, dhfR Protein, folA Protein, dfrA Protein, LOC707382 Protein, CNJ01940 Protein, DDB_G0286755 Protein, Dhfr Protein, Mesil_0096 Protein, Arch_0408 Protein, Fbal_3367 Protein, Ilyop_0482 Protein, Lbys_2007 Protein, Riean_0733 Protein, Mrub_1855 Protein, Poras_0766 Protein, Halhy_3552 Protein, Mesop_3614 Protein, LOC609048 Protein

Background: Dihydrofolate reductase (DHFR) is a member of the reductase family of enzymes that is ubiquitously expressed in all organisms. DHFR catalyzes the NADPH-dependent reduction of dihydrofolate to tetrahydrofolate, and it is essential for the synthesis of thymidylate, purines and several amino acids. Expression of methotrexate (MTX)-resistant variants of DHFR in normal hematopoietic cells is a potential strategy to permit administration of larger doses of MTX by alleviating drug toxicity in normal cells and tissues that are drug sensitive. Recombinant mouse DHFR protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: Dihydrofolate reductase, EC 1.5.1.3. NCBI no.: NP_034179

Molecular Weight: 23.8 kDa (207aa), confirmed by MALDI-TOF

Pathways: Mitotic G1-G1/S Phases