TWF1 Protein (AA 1-252) (His tag)

Catalog No. ABIN667989

Product Details

Target: TWF1 (Twinfilin, Actin-Binding Protein 1 (TWF1))

Protein Type: Recombinant

Protein Characteristics: AA 1-252

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This TWF1 protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Characteristics: TWF1, 1-252aa, Human, His tag, E.coli

Purity: > 80 % by SDS - PAGE

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/ml (determined by Bradford assay)

Buffer: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT

Storage: 4 °C

Target Details

Target: TWF1 (Twinfilin, Actin-Binding Protein 1 (TWF1))

Alternative Name: TWF1 (TWF1 Products)

Synonyms: A6 Protein, PTK9 Protein, Ptk9 Protein, twinfilin Protein, ptk9 Protein, twf1 Protein, wu:fd02b03 Protein, zgc:65922 Protein, DKFZp469O1925 Protein, CG3771 Protein, Dmel\\CG3771 Protein, EG:9D2.3 Protein, zgc:92472 Protein, twinfilin actin binding protein 1 Protein, twinfilin actin-binding protein 1a Protein, twinfilin actin binding protein 1 S homeolog Protein, twinfilin-1 Protein, hypothetical protein Protein, Twinfilin-1 Protein, twinfilin actin-binding protein 1 Protein, CG3771 gene product from transcript CG3771-RC Protein, twinfilin actin-binding protein 1b Protein, TWF1 Protein, Twf1 Protein, twf1a Protein, twf1.S Protein, PTRG_09288 Protein, PGTG_01788 Protein, Tsp_02530 Protein, twf1 Protein, a6 Protein, twf1b Protein

Background: TWF1 is a ubiquitous actin-monomer-binding protein that is composed of two ADF-homology domains. It forms a 1:1 complex with ADP-actin-monomers, inhibits nucleotide exchange on actin monomers and prevents assembly of the monomer into filaments. This protein composed of two ADF/cofilin-like (ADF-H) domains connected by a short linker region and followed by a C-terminal tail of 20 residues. The two ADF-H domains are 20% homologous to ADF/cofilin and to each other. Recombinant human TWF1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. Synonyms: Twinfilin-1, PTK9. NCBI no.: AAH22344

Molecular Weight: 31.5 kDa (277aa) confirmed by MALDI-TOF

Pathways: Regulation of Actin Filament Polymerization, Maintenance of Protein Location