IL-1 beta Protein

Catalog No. ABIN935468

This Recombinant IL-1 beta protein is produced in Escherichia coli (E. coli).

Quick Overview for IL-1 beta Protein (ABIN935468)

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 98 % pure

Product Details

Sequence: APVRSLNCTL RDSQQKSLVM SGPYELKALH LQGQDMEQQV VFSMSFVQGE ESNDKIPVAL GLKEKNLYLS CVLKDDKPTL QLESVDPKNY PKKKMEKRFV FNKIEINNKL EFESAQFPNW YISTSQAENM PVFLGGTKGG QDITDFTMQF VSS

Characteristics: Purified recombinant Human IL1 beta protein
Expression System: E.coli
Bioactivity: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by Mouse D10S cells is ? 1.0 pg/mL, corresponding to a specific activity of ? 1 x 10^9 units/mg.

Sterility: 0.2 μm filtered

Endotoxin Level: < 0.1 ng per µg (1 EU/µg).

Application Details

Application Notes: Each Investigator should determine their own optimal working dilution for specific applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in water to a concentration of 0.1-1.0 mg/mL. When dissolved, dilute further into aqueous buffered solutions.

Buffer: Sterile filtered through a 0.2 µm filter. Lyophilized from 10 mM Tris, pH 8.2.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: RT/-20 °C

Target Details

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Alternative Name: IL1 beta

Background: IL1beta is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL1a and IL1beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL2 release, B-cell maturation and proliferation, mitogenic FGFlike activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL1beta is a secreted cytokine, IL1a is predominantly a cell-associated cytokine.
Alternative Names: IL1, Leukocyte Endogenous Mediator protein, Lymphocyte-activating factor protein, LAF protein, Mononuclear Cell Factor protein, MCF protein, Catabolin protein, IL 1, IL-1 protein, Interleukin 1 beta protein, IL 1 protein, Endogenous Pyrogen protein, EP protein, LEM protein, IL-1 beta protein, IL-1

Molecular Weight: 17.3 kDa

Pathways: NF-kappaB Signaling, Interferon-gamma Pathway, TLR Signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome