IL-1 beta Protein

Catalog No. ABIN7321252

This Recombinant IL-1 beta protein is produced in Escherichia coli (E. coli).

Quick Overview for IL-1 beta Protein (ABIN7321252)

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Protein Type: Recombinant

Biological Activity: Active

Origin: Rat

Source: Escherichia coli (E. coli)

Application: Cell Culture (CC)

Purity: > 98 % as determined by reducing SDS-PAGE.

Product Details

Purpose: Recombinant Rat IL-1 beta/IL1B Protein (mature form)

Sequence: Val 117-Ser 268

Characteristics: A DNA sequence encoding the mature form of rat IL1B (Q63264-1) (Val 117-Ser 268) was expressed and purified.

Sterility: 0.2 μm filtered

Biological Activity Comment: 1. Measured in a cell proliferation assay using D10.G4. 1 mouse helper T cells. The ED₅₀ for this effect is typically 30-120 pg/mL. 2. Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The EC₅₀ for this effect is typically 20-100ng/mL.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile 40 mM Tris, 0.15 M NaCl, pH 7.8
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Alternative Name: IL-1 beta/IL1B

Background: IL1F,catabolin,Il-1b,IL1-BETA,IL-1beta,IL1F2,Il1b,Interleukin-1 beta,IL-1 beta,Interleukin-1 beta (IL1 beta or IL1B) also known as catabolin, is a member of the interleukin 1 cytokine family. IL1 is a pleiotropic cytokine. It is involved in the inflammatory response, cell growth, and tissue repair in the cortex. The IL1 superfamily consists of three members, IL1A (IL1 alpha), IL1B (IL1 beta), and IL1 receptor antagonist (IL1Ra). In clinical, it has been reported that Interleukin (IL)-1 may influence Th1 / Th2 immune responsiveness and has been implicated in the establishment of successful pregnancy. Proinflammatory interleukin (IL)-1 gene polymorphisms associated with high levels of IL-1beta activity increase the risk for hypochlorhydria and distal gastric carcinoma. IL1B polymorphisms may be involved in susceptibility to SSc. Moreover, the IL2-384-G allele may be a marker for the limited phenotype of systemic sclerosis (SSc).

Molecular Weight:

Calculated MW: 17.4 kDa

Observed MW: 35 kDa

UniProt: Q63264

Pathways: NF-kappaB Signaling, Interferon-gamma Pathway, TLR Signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome