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DARPP32 antibody (N-Term)
PPP1R1B
Reactivity: Rat
WB, IHC
Host: Rabbit
Polyclonal
unconjugated
Product Details anti-DARPP32 Antibody
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Target
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DARPP32 (PPP1R1B)
(Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B))
Binding Specificity
All epitopes for DARPP32 antibodies
N-Term
Reactivity
All reactivities for DARPP32 antibodies
Rat
Host
All hosts for DARPP32 antibodies
Rabbit
Clonality
All clonalities for DARPP32 antibodies
Polyclonal
Conjugate
All conjugates for DARPP32 antibodies
This DARPP32 antibody is un-conjugated
Application
All applications for DARPP32 antibodies
Western Blotting (WB), Immunohistochemistry (IHC)
Specificity
Specific for the ~32k DARPP-32 protein.
Cross-Reactivity
Rat (Rattus)
Predicted Reactivity
bovine, canine, chicken, human, mouse, non-human primate, Xenopus
Purification
Antigen Affinity Purified from Pooled Serum
Immunogen
Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH
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Alternatives
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Application Details
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Application Notes
Recommended Dilution: WB: 1:1000 IHC: 1:250 Quality Control: Western blots performed on each lot.
Restrictions
For Research Use only
Handling
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Format
Liquid
Buffer
100 μL in 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.
Storage
-20 °C
References for anti-DARPP32 antibody (ABIN361423)
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Gomez, Midde, Mactutus, Booze, Zhu : "Environmental enrichment alters nicotine-mediated locomotor sensitization and phosphorylation of DARPP-32 and CREB in rat prefrontal cortex. " in: PLoS ONE , Vol. 7 , Issue 8 , pp. e44149 , (2013 ) (PubMed ).
Target Details for DARPP32
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Target
DARPP32 (PPP1R1B)
(Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B))
Alternative Name
PPP1R1B (PPP1R1B Products )
Synonyms
darpp32 antibody, darpp-32 antibody, Darpp-32 antibody, Darpp32 antibody, DARPP-32 antibody, DARPP32 antibody, AU040756 antibody, protein phosphatase 1 regulatory inhibitor subunit 1B antibody, protein phosphatase 1, regulatory (inhibitor) subunit 1B antibody, protein phosphatase 1 regulatory inhibitor subunit 1B S homeolog antibody, ppp1r1b antibody, PPP1R1B antibody, Ppp1r1b antibody, ppp1r1b.S antibody
Background
DARPP-32 is a dopamine (DA) and cAMP-regulated ~32k phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998). The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA (Bibb et al., 1999). Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac® (Maldve et al., 2002, Lindskog et al., 2002, Svenningsson et al., 2002). Anti-DARPP-32 Western blot of a rat hippocampal lysate showing specific immunolabeling of the ~32k DARPP protein.
Molecular Weight
'32 kDa
Gene ID
360616
UniProt
Q6J4I0
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