CD25 antibody (FITC)

Catalog No. ABIN6655112

Product Details anti-CD25 Antibody (FITC)

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD25 antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC)

Cross-Reactivity (Details): Cross reactivity with CD25 from other sources has not been tested

Purification: Fluorescein conjugated CD25 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD25. Reactivity is observed against human CD25, Baboon, Chimpanzee, Cynomolgus, Rhesus, and Pigtailed Macaque. . Anti-CD25 is conjugated with FITC under optimal conditions and the solution is free of unconjugated FITC.

Immunogen:

Immunogen: Anti-CD25 Antibody (Monoclonal) was produced by repeated immunizations with CD25 antigen.

Clone: BC96

Isotype: IgG1

Labeling Ratio: 4-6

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-CD25 is useful for Flow Cytometry and Immunofluorescence. Researchers should determine optimal titers for applications that are not stated.

Flow Cytometry Dilution: 5 µL/1x10e6 cells or 100μL of whole blood

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.2 % BSA (w/v)

0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.

References for anti-CD25 antibody (FITC) (ABIN6655112)

Taniguchi, Minami: "The IL-2/IL-2 receptor system: a current overview." in: Cell, Vol. 73, Issue 1, pp. 5-8, (1993) (PubMed).

Waldmann: "The interleukin-2 receptor." in: The Journal of biological chemistry, Vol. 266, Issue 5, pp. 2681-4, (1991) (PubMed).

Target Details for CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Alternative Name: CD25 (IL2RA Products)

Synonyms: CD25 antibody, IDDM10 antibody, IL2R antibody, TCGFR antibody, IL2RAC antibody, IL-2RA antibody, Il2r antibody, Ly-43 antibody, interleukin 2 receptor subunit alpha antibody, interleukin-2 receptor subunit alpha-like antibody, interleukin 2 receptor, alpha chain antibody, IL2RA antibody, Il2ra antibody, LOC100222957 antibody

Background:

Synonyms: Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, TAC antigen, p55

Background: CD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.

Gene Name: IL2RA

Gene ID: 3559

NCBI Accession: NP_000408

UniProt: P01589

Pathways: JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation