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|Application / Reactivity||Human||Mouse (Murine)|
|Western Blotting (WB)||42 Antibodies||9 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Neutralization (Neut)||1 Antibodies|
|Immunoprecipitation (IP)||15 Antibodies||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||10 Antibodies||4 Antibodies|
|Immunohistochemistry (IHC)||19 Antibodies||5 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies||2 Antibodies|
|Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||2 Antibodies||2 Antibodies|
|Immunofluorescence (IF)||4 Antibodies|
|Immunocytochemistry (ICC)||4 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Antigen||Amphiregulin (AREG) Antibodies|
|Epitope||AA 8-26 Alternatives|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This Amphiregulin antibody is un-conjugated Alternatives|
|2 references available|
|Supplier||Log in to see|
Product Details anti-Amphiregulin AntibodyTarget Details Amphiregulin Application Details Handling References for anti-Amphiregulin Antibody (ABIN181313) Images
Highly specific for Amphiregulin.
Cellular Localization: Cytoplasmic and/or nuclear. Mol. Wt. of Antigen: 18.5-22.5 kDa.
|Purification||IgG purified from rabbit anti-serum by Protein A chromatography.|
|Immunogen||A synthetic 19-mer peptide, corresponding to aa 8-26 (VKPPQNKTESENTSDKPKR) from the secreted amphiregulin of human origin.|
Target Details AmphiregulinProduct Details anti-Amphiregulin Antibody Application Details Handling References for anti-Amphiregulin Antibody (ABIN181313) Images back to top
|Alternative Name||Amphiregulin (AREG Antibody Abstract)|
|Background||Amphiregulin (AR) binds to EGF-Receptor (EGFR) with lower affinity than EGF. The mature secreted form AR is an 84-amino acid residue glycosylated polypeptide growth regulator, which is generated by proteolytic processing of a 252-amino acid transmembrane precursor. Seven different polypeptide ligands, which derive from distinct genes, are capable of binding to the extracellular domain of EGFR. These ligands include EGF, TGFalpha, AR, HB-EGF, cripto-1, epiregulin, and betacellulin. All of these growth factors contain a characteristic EGF-like domain which is defined by 6 evenly spaced cysteine residues that generate 3 loops through the formation of disulfide bonds. AR protein has been localized by immunohistochemistry to the epithelium of the colon, stomach, pancreas, breast, and placenta. AR is reportedly overexpressed in human cancers of breast, colon, stomach, and pancreas.Synonyms: AREG, CRDGF, Colorectum cell-derived growth factor, SDGF, Schwannoma-Derived Growth Factor|
|Research Area||Cancer, Growth Factors|
|Pathways||RTK Signaling, EGFR Signaling Pathway|
Application DetailsProduct Details anti-Amphiregulin Antibody Target Details Amphiregulin Handling References for anti-Amphiregulin Antibody (ABIN181313) Images back to top
Immunoprecipitation (Native): use Protein A and Ab at 10 g/mg protein lysate. PositiveControl: LS174T cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Amphiregulin Antibody Target Details Amphiregulin Application Details References for anti-Amphiregulin Antibody (ABIN181313) Images back to top
|Buffer||10 mM PBS, pH 7.4, with 0.2 % BSA and 0.09 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid Repeated freeze/thaw cycles.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store at 2-8 °C for up to one month or in aliquots at -20 °C for longer.|
References for anti-Amphiregulin Antibody (ABIN181313)Product Details anti-Amphiregulin Antibody Target Details Amphiregulin Application Details Handling Images back to top
Johnson, Prigent, Gullick et al.: "Characterization of high and low molecular weight forms of amphiregulin that differ in glycosylation and peptide core length. Evidence that the NH2-terminal region is not critical for bioactivity." in: The Journal of biological chemistry, Vol. 268, Issue 25, pp. 18835-43, 1993 (PubMed).
Johnson, Saeki, Gordon et al.: "Autocrine action of amphiregulin in a colon carcinoma cell line and immunocytochemical localization of amphiregulin in human colon." in: The Journal of cell biology, Vol. 118, Issue 3, pp. 741-51, 1992 (PubMed).