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anti-Human Amphiregulin antibody for Immunoprecipitation

Recommended Amphiregulin Antibody (supplied by: Log in to see )

Antigen
Amphiregulin (AREG) Antibodies
  • AR
  • AREG
  • AREG/SDGF
  • AREGB
  • CRDGF
  • SDGF
  • Sdgf
Epitope
AA 8-26
12
8
5
4
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
67
12
5
2
1
1
1
Host
Rabbit
58
10
2
1
1
1
Clonality
Polyclonal
Conjugate
This Amphiregulin antibody is un-conjugated
1
1
Application
Immunoprecipitation (IP)
46
26
18
13
11
6
5
4
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN181313
$ 531.30
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089235755 ABIN153430 IP Rabbit Log in to see Polyclonal
0.00089235755 ABIN387285 IP Rabbit IgG AA 8-26 Log in to see Polyclonal
0.00089235755 ABIN2200220 IP ELISA Rabbit IgG AA 8-26 Log in to see Polyclonal
0.00089235755 ABIN2200227 IP WB Rabbit IgG AA 26-44 Log in to see Polyclonal
0.00089235755 ABIN387291 IP Rabbit IgG AA 26-44 Log in to see Polyclonal
0.00089235755 ABIN2200226 IP ELISA Rabbit IgG AA 8-26 Log in to see Polyclonal
0.00089235755 ABIN387286 IP Rabbit IgG AA 8-26 Log in to see Polyclonal
0.00089235755 ABIN387293 IP WB Rabbit IgG AA 26-44 Log in to see Polyclonal
0.00089235755 ABIN2200228 IP WB Rabbit IgG AA 26-44 Log in to see Polyclonal
0.00089235755 ABIN2200234 IP IHC Rabbit IgG AA 8-26 Log in to see Polyclonal
0.00089235755 ABIN387288 IP Rabbit IgG AA 8-26 Log in to see Polyclonal
0.00089235755 ABIN2165344 IHC (fp) IP Rabbit IgG Log in to see Polyclonal
0.00089235755 ABIN2200225 IP Rabbit AA 8-26 Log in to see Polyclonal
0.00089235755 ABIN2477406 IHC (p) IP Rabbit IgG AA 8-26 Log in to see Polyclonal

Top referenced anti-Amphiregulin antibody for Immunoprecipitation

Similar anti-Amphiregulin Antibodies

Application / Reactivity Human Mouse (Murine)
Western Blotting (WB) 42 Antibodies 9 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Neutralization (Neut) 1 Antibodies
Immunoprecipitation (IP) 15 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 10 Antibodies 4 Antibodies
Immunohistochemistry (IHC) 19 Antibodies 5 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies 2 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies 1 Antibodies
Immunofluorescence (IF) 4 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunochromatography (IC) 1 Antibodies

General

Antigen Amphiregulin (AREG) Antibodies
Epitope AA 8-26
(12), (8), (5), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(67), (12), (5), (2), (1), (1), (1)
Host Rabbit
(58), (10), (2), (1), (1), (1)
Clonality
Polyclonal
Conjugate This Amphiregulin antibody is un-conjugated
(1), (1)
Application Immunoprecipitation (IP)
(46), (26), (18), (13), (11), (6), (5), (4), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-Amphiregulin Antibody

Target Details Amphiregulin Application Details Handling References for anti-Amphiregulin Antibody (ABIN181313) Images
Specificity Highly specific for Amphiregulin.
Cellular Localization: Cytoplasmic and/or nuclear. Mol. Wt. of Antigen: 18.5-22.5 kDa.
Purification IgG purified from rabbit anti-serum by Protein A chromatography.
Immunogen A synthetic 19-mer peptide, corresponding to aa 8-26 (VKPPQNKTESENTSDKPKR) from the secreted amphiregulin of human origin.

Target Details Amphiregulin

Product Details anti-Amphiregulin Antibody Application Details Handling References for anti-Amphiregulin Antibody (ABIN181313) Images back to top
Antigen
Alternative Name Amphiregulin (AREG Antibody Abstract)
Background Amphiregulin (AR) binds to EGF-Receptor (EGFR) with lower affinity than EGF. The mature secreted form AR is an 84-amino acid residue glycosylated polypeptide growth regulator, which is generated by proteolytic processing of a 252-amino acid transmembrane precursor. Seven different polypeptide ligands, which derive from distinct genes, are capable of binding to the extracellular domain of EGFR. These ligands include EGF, TGFalpha, AR, HB-EGF, cripto-1, epiregulin, and betacellulin. All of these growth factors contain a characteristic EGF-like domain which is defined by 6 evenly spaced cysteine residues that generate 3 loops through the formation of disulfide bonds. AR protein has been localized by immunohistochemistry to the epithelium of the colon, stomach, pancreas, breast, and placenta. AR is reportedly overexpressed in human cancers of breast, colon, stomach, and pancreas.Synonyms: AREG, CRDGF, Colorectum cell-derived growth factor, SDGF, Schwannoma-Derived Growth Factor
Molecular Weight 18.5-22.5kDa.
Gene ID 374
NCBI Accession NP_001648
UniProt P15514
Research Area Cancer, Growth Factors
Pathways RTK Signaling, EGFR Signaling Pathway

Application Details

Product Details anti-Amphiregulin Antibody Target Details Amphiregulin Handling References for anti-Amphiregulin Antibody (ABIN181313) Images back to top
Application Notes Immunoprecipitation (Native): use Protein A and Ab at 10 g/mg protein lysate. PositiveControl: LS174T cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Amphiregulin Antibody Target Details Amphiregulin Application Details References for anti-Amphiregulin Antibody (ABIN181313) Images back to top
Buffer 10 mM PBS, pH 7.4, with 0.2 % BSA and 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid Repeated freeze/thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Store at 2-8 °C for up to one month or in aliquots at -20 °C for longer.

References for anti-Amphiregulin Antibody (ABIN181313)

Product Details anti-Amphiregulin Antibody Target Details Amphiregulin Application Details Handling Images back to top
Background publications

Johnson, Prigent, Gullick, Stromberg: "Characterization of high and low molecular weight forms of amphiregulin that differ in glycosylation and peptide core length. Evidence that the NH2-terminal region is not critical for bioactivity." in: The Journal of biological chemistry, Vol. 268, Issue 25, pp. 18835-43, 1993

Johnson, Saeki, Gordon, Shoyab, Salomon, Stromberg: "Autocrine action of amphiregulin in a colon carcinoma cell line and immunocytochemical localization of amphiregulin in human colon." in: The Journal of cell biology, Vol. 118, Issue 3, pp. 741-51, 1992