anti-Rabbit BDNF antibody for Immunocytochemistry

Recommended BDNF Antibody (supplied by: Log in to see )

Antigen
Brain-Derived Neurotrophic Factor (BDNF) Antibodies
  • bdnf-A
  • BDNF
  • Bdnf
  • bdnf
  • ANON2
  • BULN2
  • brain-derived neurotrophic factor
  • brain derived neurotrophic factor
  • brain-derived neurotrophic factor-like
  • bdnf
  • bdnf-A
  • BDNF
  • Bdnf
  • bdnf-b
  • LOC100356949
Epitope
AA 16-183
36
30
18
9
8
7
6
6
6
5
5
5
5
4
4
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Rabbit
321
133
114
18
16
13
11
7
6
5
5
4
3
3
2
2
Host
Guinea Pig
225
117
11
11
10
4
Clonality
Polyclonal
Conjugate
This BDNF antibody is un-conjugated
31
20
13
7
7
6
6
6
5
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
288
149
111
69
67
55
54
23
13
10
9
4
4
4
2
2
1
1
1
1
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Catalog No. ABIN1858131
$ 342.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN5013241 ICC IHC IP WB Rabbit IgG AA 20-252 Log in to see Polyclonal

General

Antigen Brain-Derived Neurotrophic Factor (BDNF) Antibodies
Epitope AA 16-183
(36), (30), (18), (9), (8), (7), (6), (6), (6), (5), (5), (5), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Rabbit
(321), (133), (114), (18), (16), (13), (11), (7), (6), (5), (5), (4), (3), (3), (2), (2)
Host Guinea Pig
(225), (117), (11), (11), (10), (4)
Clonality
Polyclonal
Conjugate This BDNF antibody is un-conjugated
(31), (20), (13), (7), (7), (6), (6), (6), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(288), (149), (111), (69), (67), (55), (54), (23), (13), (10), (9), (4), (4), (4), (2), (2), (1), (1), (1), (1)
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Product Details anti-BDNF Antibody

Target Details BDNF Application Details Handling Images
Sequence MGHHHHHHSG SEF-MVISY FGCMKAAPMK EANVRGQGSL AYPGMRTHGT LESVNGPKTG SRGLTSLADT FEHVIEELLD EDQKVRPNEE NNKDADLYTS RVMLSSQVPL EPPLLFLLEE YKNYLDAANM SMRVRRHSDP ARRGELSVCD SISEWVTAAD KKTAVDMSGG TVTVLEKVPV SKG
Specificity It has been selected for its ability to recognize BDNF in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen BDNF (AA 16-183)
Isotype IgG

Target Details BDNF

Product Details anti-BDNF Antibody Application Details Handling Images back to top
Antigen
Alternative Name Brain Derived Neurotrophic Factor (BDNF) (BDNF Antibody Abstract)
Research Area Hormones
Pathways RTK Signaling, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity

Application Details

Product Details anti-BDNF Antibody Target Details BDNF Handling Images back to top
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Comment

Content: The quality control contains recombinant BDNF (Met16~Gly183) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions For Research Use only

Handling

Product Details anti-BDNF Antibody Target Details BDNF Application Details Images back to top
Format Liquid
Concentration 200 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-BDNF Antibody Target Details BDNF Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Brain-Derived Neurotrophic Factor (BDNF) (AA 16-183) antibody (ABIN1858131) anti-Brain-Derived Neurotrophic Factor (BDNF) (AA 16-183) antibody