anti-Human EPH Receptor B4 antibody for Immunofluorescence

Recommended EPH Receptor B4 Antibody (supplied by: Log in to see )

Antigen
EPH Receptor B4 (EPHB4) Antibodies
  • htk
  • myk1
  • ephB4a
  • tyro11
  • ephb4b
  • MGC130997
  • AI042935
  • Htk
  • MDK2
  • Myk1
  • Tyro11
  • HTK
  • MYK1
  • TYRO11
  • EPH receptor B4
  • Eph receptor B4
  • EPHB4
  • ephb4-a
  • ephb4
  • LOC100359286
  • Ephb4
Epitope
AA 1-539
20
17
17
14
12
7
6
5
4
3
3
3
3
2
1
1
1
1
Reactivity
Human
148
67
10
1
1
1
1
Host
Mouse
69
66
38
18
2
Clonality
Monoclonal
Conjugate
This EPH Receptor B4 antibody is conjugated to FITC
7
6
5
5
5
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunofluorescence (fixed cells) (IF/ICC)
137
106
34
26
21
19
5
4
3
3
2
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN1996162
$ 198.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.32492 ABIN1996164 FACS IF IF/ICC PE Mouse IgG1 AA 1-539 Log in to see 04
12.32492 ABIN614445 EIA IF IHC (p) WB Mouse IgG1 Extracellular Domain Log in to see 5B5
12.32492 ABIN1107115 IF WB Mouse IgG1 Log in to see 5B5 2
12.32492 ABIN4308715 ELISA ICC IF WB Mouse IgG1 Log in to see 5B5
12.32492 ABIN931422 IF ELISA WB Mouse IgG1 Log in to see 5B5
1 ABIN462588 IF IHC (p) ELISA WB Mouse IgG1 Extracellular Log in to see 5B5
1 ABIN3184512 ELISA IF IHC WB Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN498286 IF IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN2470840 IF IHC ELISA WB Mouse IgG1 Log in to see 5B5
1 ABIN1996159 ELISA FACS IF IF/ICC Mouse IgG1 AA 1-539, Extracellular Domain Log in to see 04
1 ABIN966075 IF ELISA WB Mouse IgG2a Log in to see 5B8F7 2
1 ABIN5577412 IF IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN2260864 IF IHC ELISA WB Mouse IgG1 Log in to see 5B5
1 ABIN1582826 IF WB Mouse IgG1 Log in to see 5B5
1 ABIN5577409 ELISA IF WB Mouse IgG1 Log in to see 5B5
1 ABIN4901468 ELISA IF WB Mouse IgG1 Log in to see 5B5
1 ABIN1576319 IF ELISA WB Mouse Log in to see

Top referenced anti-EPH Receptor B4 antibody for Immunofluorescence

Similar anti-EPH Receptor B4 Antibodies

Application / Reactivity Human
ELISA 88 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 30 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunofluorescence (IF) 18 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 4 Antibodies
Immunohistochemistry (IHC) 21 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 18 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Simple Western (SimWes) 1 Antibodies
Western Blotting (WB) 97 Antibodies

General

Antigen EPH Receptor B4 (EPHB4) Antibodies
Epitope AA 1-539
(20), (17), (17), (14), (12), (7), (6), (5), (4), (3), (3), (3), (3), (2), (1), (1), (1), (1)
Reactivity Human
(148), (67), (10), (1), (1), (1), (1)
Host Mouse
(69), (66), (38), (18), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This EPH Receptor B4 antibody is conjugated to FITC
(7), (6), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunofluorescence (fixed cells) (IF/ICC)
(137), (106), (34), (26), (21), (19), (5), (4), (3), (3), (2), (1), (1), (1)
Supplier Log in to see

Product Details anti-EPH Receptor B4 Antibody

Target Details EPH Receptor B4 Application Details Handling Images
Specificity Human EphB4
No cross-reactivity in WB and ELISA with
Human cell lysate (293 cell line)
No Cross-Reactivity Human
Characteristics This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human EphB4 (rhEphB4, ABIN2002403 NP_004435.3, Met 1-Ala 539, and conjugated with FITC under optimum conditions, the unreacted FITC was removed.
Immunogen Recombinant Human EphB4 protein (ABIN2002403)
Clone 04
Isotype IgG1

Target Details EPH Receptor B4

Product Details anti-EPH Receptor B4 Antibody Application Details Handling Images back to top
Antigen
Alternative Name EPHB4 (EPHB4 Antibody Abstract)
Background EphB4, also known as Htk, Myk-1, is a member of the Eph subfamily of receptor tyrosine kinases (RTKs). The Ephs are divided into two classes based on their extracellular structure and ligand specificity, and the transmembrane receptor binding preferentially to ephrin-B ligands is referred to as EphB. Sharing common structure organization, all Eph receptors contain an N-terminal Ig-like domain, a cysteine-rich region and two fibronectin type III domains within the ECD, as well as the typical cytoplasmic tyrosine kinase domain. EphB4 and its ligand ephrin-B2 are specifically expressed on venous and arterial endothelial cells, respectively, and play an essential role in vascular development via bidirectional signals. The forward EphB4 signaling inhibits cell adhesion, chemotaxis, angiogenesis, and tumor growth. In contrast, the reverse Ephrin-B2 signaling exerts the opposite effect. It has been reported that aberrant expression of EphB4 is associated with prostate cancer and highly malignant breast cancers, and accordingly, EphB4 has potential applications as a therapeutic candidate.
NCBI Accession NP_004435.3
Pathways RTK Signaling

Application Details

Product Details anti-EPH Receptor B4 Antibody Target Details EPH Receptor B4 Handling Images back to top
Application Notes ICC/IF: 5-20 μg/mL
Restrictions For Research Use only

Handling

Product Details anti-EPH Receptor B4 Antibody Target Details EPH Receptor B4 Application Details Images back to top
Format Liquid
Concentration 0.1 mg/mL
Buffer Aqueous solution containing 0.5 % BSA and 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal
Storage 4 °C
Storage Comment This antibody is stable for 12 months from date of receipt when stored at 2°C-8°C. Protected from prolonged exposure to light. Do not freeze !
Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
Expiry Date 12 months

Images

Product Details anti-EPH Receptor B4 Antibody Target Details EPH Receptor B4 Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-EPH Receptor B4 antibody (EPHB4) (AA 1-539) (FITC) (ABIN1996162) Profile of anti-EphB4 reactivity on MCF-7 cells analyzed by flow cytometry.
Immunofluorescence (IF) image for anti-EPH Receptor B4 antibody (EPHB4) (AA 1-539) (FITC) (ABIN1996162) Immunofluorescence staining of Human EphB4 in MCF7 cells. Cells were fixed with 4% PF...
Flow Cytometry (FACS) image for anti-EPH Receptor B4 antibody (EPHB4) (AA 1-539) (FITC) (ABIN1996162) EphB4 Antibody (APC), Mouse MAb, Flow cytometric analysis