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anti-Human FGF2 antibody for Blocking Antibody

Recommended FGF2 Antibody (supplied by: Log in to see )

Antigen
Fibroblast Growth Factor 2 (Basic) (FGF2) Antibodies
  • bFGF
  • BFGF
  • Fgf-2
  • FGF-2
  • Fgfb
  • FGFB
  • HBGF-2
Reactivity
Human
300
57
43
38
16
9
5
3
1
1
1
1
Host
Rabbit
180
147
7
6
1
1
Clonality
Polyclonal
Conjugate
This FGF2 antibody is un-conjugated
30
18
13
8
5
5
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Blocking Antibody (Inhibition), ELISA, Western Blotting (WB)
240
207
97
56
34
27
21
20
17
11
11
11
7
5
3
2
2
1
1
1
1
1
1
1
1
1
1
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Catalog No. ABIN1001864
$ 374.22
Plus shipping costs $45.00

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General

Antigen Fibroblast Growth Factor 2 (Basic) (FGF2) Antibodies
Reactivity Human
(300), (57), (43), (38), (16), (9), (5), (3), (1), (1), (1), (1)
Host Rabbit
(180), (147), (7), (6), (1), (1)
Clonality
Polyclonal
Conjugate This FGF2 antibody is un-conjugated
(30), (18), (13), (8), (5), (5), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Blocking Antibody (Inhibition), ELISA, Western Blotting (WB)
(240), (207), (97), (56), (34), (27), (21), (20), (17), (11), (11), (11), (7), (5), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-FGF2 Antibody

Target Details FGF2 Application Details Handling Images
Purification Antigen affinity purified polyclol antibody
Sterility Sterile filtered
Immunogen recombint hFGF-basic (human Fibroblast Growth Factor-basic)
Isotype IgG

Target Details FGF2

Product Details anti-FGF2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name basic Fibroblast Growth Factor (FGF2 Antibody Abstract)
Background Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hFGF-basic (human Fibroblast Growth Factor-basic). Anti-Human FGF-basic specific antibody was purified by affinity chromatography employing immobilized hFGF-basic matrix.
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-FGF2 Antibody Target Details FGF2 Handling Images back to top
Application Notes To yield one-half maximal inhibition [ND₅₀] of the biological activity of hFGF-basic (0.30 ng/µl), a concentration of 0.25 - 0.40 µg/µl of this antibody is required. To detect hFGF-basic by indirect ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFGF-basic. To detect hFGF-basic by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PromoKine's Biotinylated Anti-Human FGF-basic (PK-AB815-60240B) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFGF-basic. To detect hFGF-basic by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFGF-basic is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Immunihostochemistry: NA.
Restrictions For Research Use only

Handling

Product Details anti-FGF2 Antibody Target Details FGF2 Application Details Images back to top
Format Lyophilized
Buffer A sterile-filtered antibody solution was lyophilized from PBS, pH 7.2.
Handling Advice The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles. Centrifuge vial prior to opening. Reconstitute in sterile water at a concentration of 0.1-1.0 mg/ml.
Storage -20 °C
Expiry Date 24 months