|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)||Opossum (Didelphimorphia)||Guinea Pig|
|Western Blotting (WB)||169 Antibodies||29 Antibodies||20 Antibodies||1 Antibodies||2 Antibodies|
|Transfected Cell Culture (TCC)||1 Antibodies|
|Radioimmunoassay (RIA)||3 Antibodies||2 Antibodies||2 Antibodies|
|Radial Immunodiffusion (RID)||1 Antibodies|
|Neutralization (Neut)||21 Antibodies|
|Immunostaining (ISt)||1 Antibodies|
|Immunoprecipitation (IP)||21 Antibodies||2 Antibodies||4 Antibodies|
|Immunohistochemistry (Paraformaldehyde) (IHC (pfa))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||29 Antibodies||5 Antibodies||6 Antibodies||3 Antibodies|
|Antigen||Fibroblast Growth Factor 2 (Basic) (FGF2) Antibodies|
|Reactivity||Opossum (Didelphimorphia), Mouse (Murine), Guinea Pig, Rat (Rattus), Human Alternatives|
|Conjugate||This FGF2 antibody is un-conjugated Alternatives|
Immunohistochemistry (IHC), Western Blotting (WB)
|7 references available|
|Supplier||Log in to see|
Product Details anti-FGF2 AntibodyTarget Details FGF2 Application Details Handling References for anti-FGF2 Antibody (ABIN350320) Images
|Specificity||Specific for FGF2.|
|Immunogen||A synthetic peptides from human basic FGF2 were used as the antigen. The peptides are homologous in many other species including rat and mouse.|
Target Details FGF2Product Details anti-FGF2 Antibody Application Details Handling References for anti-FGF2 Antibody (ABIN350320) Images back to top
|Alternative Name||FGF2 (FGF2 Antibody Abstract)|
|Background||The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. Also known as: BFGF, FGFB, HBGF-2, prostatropin.|
|Pathways||RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-FGF2 Antibody Target Details FGF2 Handling References for anti-FGF2 Antibody (ABIN350320) Images back to top
A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
|Restrictions||For Research Use only|
HandlingProduct Details anti-FGF2 Antibody Target Details FGF2 Application Details References for anti-FGF2 Antibody (ABIN350320) Images back to top
|Reconstitution||Reconstitute in 150 µL of sterile water. Centrifuge to remove any insoluble material.|
|Handling Advice||Avoid freeze and thaw cycles.|
|Storage||4 °C/-20 °C|
|Storage Comment||Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.|
|Expiry Date||12 months|
References for anti-FGF2 Antibody (ABIN350320)Product Details anti-FGF2 Antibody Target Details FGF2 Application Details Handling Images back to top
Alexander, Watt, Sawaji et al.: "Activin A is an anticatabolic autocrine cytokine in articular cartilage whose production is controlled by fibroblast growth factor 2 and NF-kappaB." in: Arthritis and rheumatism, Vol. 56, Issue 11, pp. 3715-25, 2007 (PubMed).
Neuzil, Swettenham, Wang et al.: "alpha-Tocopheryl succinate inhibits angiogenesis by disrupting paracrine FGF2 signalling." in: FEBS letters, Vol. 581, Issue 24, pp. 4611-5, 2007 (PubMed).
Arkudas, Tjiawi, Bleiziffer et al.: "Fibrin gel-immobilized VEGF and bFGF efficiently stimulate angiogenesis in the AV loop model." in: Molecular medicine (Cambridge, Mass.), Vol. 13, Issue 9-10, pp. 480-7, 2007 (PubMed).
Prats, Kaghad, Prats et al.: "High molecular mass forms of basic fibroblast growth factor are initiated by alternative CUG codons." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, Issue 6, pp. 1836-40, 1989 (PubMed).
Ago, Kitagawa, Fujishima et al.: "Crystal structure of basic fibroblast growth factor at 1.6 A resolution." in: Journal of biochemistry, Vol. 110, Issue 3, pp. 360-3, 1992 (PubMed).
Zhu, Komiya, Chirino et al.: "Three-dimensional structures of acidic and basic fibroblast growth factors." in: Science (New York, N.Y.), Vol. 251, Issue 4989, pp. 90-3, 1991 (PubMed).
Watson, Anthony, Pickett et al.: "Reverse transcription with nested polymerase chain reaction shows expression of basic fibroblast growth factor transcripts in human granulosa and cumulus cells from in vitro fertilisation patients." in: Biochemical and biophysical research communications, Vol. 187, Issue 3, pp. 1227-31, 1992 (PubMed).