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anti-Opossum (Didelphimorphia) FGF2 antibody for Western Blotting

Recommended FGF2 Antibody (supplied by: Log in to see )

Antigen
Fibroblast Growth Factor 2 (Basic) (FGF2) Antibodies
  • bFGF
  • BFGF
  • Fgf-2
  • FGF-2
  • Fgfb
  • FGFB
  • HBGF-2
Reactivity
Opossum (Didelphimorphia), Mouse (Murine), Guinea Pig, Rat (Rattus), Human
300
56
42
38
15
9
5
3
1
1
1
Host
Sheep
181
147
6
6
1
1
Clonality
Polyclonal
Conjugate
This FGF2 antibody is un-conjugated
30
18
13
8
5
5
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
240
208
96
56
34
27
21
20
17
11
11
11
7
5
3
2
2
1
1
1
1
1
1
1
1
1
1
1
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Catalog No. ABIN350320
$ 454.67
Plus shipping costs $45.00

Top referenced anti-FGF2 antibody for Western Blotting

Similar anti-FGF2 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Opossum (Didelphimorphia) Guinea Pig
Western Blotting (WB) 213 Antibodies 31 Antibodies 22 Antibodies 1 Antibodies 2 Antibodies
Transfected Cell Culture (TCC) 1 Antibodies
Radioimmunoassay (RIA) 3 Antibodies 2 Antibodies 2 Antibodies
Radial Immunodiffusion (RID) 1 Antibodies
Neutralization (Neut) 22 Antibodies
Immunostaining (ISt) 1 Antibodies
Immunoprecipitation (IP) 21 Antibodies 2 Antibodies 4 Antibodies
Immunohistochemistry (Paraformaldehyde) (IHC (pfa)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 49 Antibodies 5 Antibodies 6 Antibodies 3 Antibodies

General

Antigen Fibroblast Growth Factor 2 (Basic) (FGF2) Antibodies
Reactivity Opossum (Didelphimorphia), Mouse (Murine), Guinea Pig, Rat (Rattus), Human
(300), (56), (42), (38), (15), (9), (5), (3), (1), (1), (1)
Host Sheep
(181), (147), (6), (6), (1), (1)
Clonality
Polyclonal
Conjugate This FGF2 antibody is un-conjugated
(30), (18), (13), (8), (5), (5), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(240), (208), (96), (56), (34), (27), (21), (20), (17), (11), (11), (11), (7), (5), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 7 references available
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Product Details anti-FGF2 Antibody

Target Details FGF2 Application Details Handling References for anti-FGF2 Antibody (ABIN350320) Images
Specificity Specific for FGF2.
Purification Whole serum
Immunogen A synthetic peptides from human basic FGF2 were used as the antigen. The peptides are homologous in many other species including rat and mouse.

Target Details FGF2

Product Details anti-FGF2 Antibody Application Details Handling References for anti-FGF2 Antibody (ABIN350320) Images back to top
Antigen
Alternative Name FGF2 (FGF2 Antibody Abstract)
Background The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. Also known as: BFGF, FGFB, HBGF-2, prostatropin.
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-FGF2 Antibody Target Details FGF2 Handling References for anti-FGF2 Antibody (ABIN350320) Images back to top
Application Notes A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only

Handling

Product Details anti-FGF2 Antibody Target Details FGF2 Application Details References for anti-FGF2 Antibody (ABIN350320) Images back to top
Format Lyophilized
Reconstitution Reconstitute in 150 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months

References for anti-FGF2 Antibody (ABIN350320)

Product Details anti-FGF2 Antibody Target Details FGF2 Application Details Handling Images back to top
Background publications

Alexander, Watt, Sawaji, Hermansson, Saklatvala: "Activin A is an anticatabolic autocrine cytokine in articular cartilage whose production is controlled by fibroblast growth factor 2 and NF-kappaB." in: Arthritis and rheumatism, Vol. 56, Issue 11, pp. 3715-25, 2007

Arkudas, Tjiawi, Bleiziffer, Grabinger, Polykandriotis, Beier, Stuerzl, Horch, Kneser: "Fibrin gel-immobilized VEGF and bFGF efficiently stimulate angiogenesis in the AV loop model." in: Molecular medicine (Cambridge, Mass.), Vol. 13, Issue 9-10, pp. 480-7, 2007

Neuzil, Swettenham, Wang, Dong, Stapelberg: "alpha-Tocopheryl succinate inhibits angiogenesis by disrupting paracrine FGF2 signalling." in: FEBS letters, Vol. 581, Issue 24, pp. 4611-5, 2007

Watson, Anthony, Pickett, Lambden, Masson, Thomas: "Reverse transcription with nested polymerase chain reaction shows expression of basic fibroblast growth factor transcripts in human granulosa and cumulus cells from in vitro fertilisation patients." in: Biochemical and biophysical research communications, Vol. 187, Issue 3, pp. 1227-31, 1992

Ago, Kitagawa, Fujishima, Matsuura, Katsube: "Crystal structure of basic fibroblast growth factor at 1.6 A resolution." in: Journal of biochemistry, Vol. 110, Issue 3, pp. 360-3, 1992

Zhu, Komiya, Chirino, Faham, Fox, Arakawa, Hsu, Rees: "Three-dimensional structures of acidic and basic fibroblast growth factors." in: Science (New York, N.Y.), Vol. 251, Issue 4989, pp. 90-3, 1991

Prats, Kaghad, Prats, Klagsbrun, Luelias, Liauzun, Chalon, Tauber, Amalric, Smith: "High molecular mass forms of basic fibroblast growth factor are initiated by alternative CUG codons." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, Issue 6, pp. 1836-40, 1989