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anti-Zebrafish (Danio rerio) FGF2 antibody for Immunohistochemistry

Recommended FGF2 Antibody (supplied by: Log in to see )

Antigen
Fibroblast Growth Factor 2 (Basic) (FGF2) Antibodies
  • bFGF
  • BFGF
  • Fgf-2
  • FGF-2
  • Fgfb
  • FGFB
  • HBGF-2
Epitope
Middle Region
14
13
12
12
10
8
8
6
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
255
54
39
17
16
8
4
2
1
1
Host
Rabbit
175
107
6
6
1
1
Clonality
Polyclonal
Conjugate
This FGF2 antibody is un-conjugated
28
16
11
6
5
5
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
194
167
90
34
25
21
19
17
14
11
11
10
6
5
3
2
1
1
1
1
1
1
1
1
1
1
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Catalog No. ABIN2777106
$ 289.00
Plus shipping costs $45.00

Top referenced anti-FGF2 antibody for Immunohistochemistry

Similar anti-FGF2 Antibodies

Application / Reactivity Cow (Bovine) Human Mouse (Murine) Rat (Rattus) Chicken Dog (Canine) Rabbit Zebrafish (Danio rerio)
Western Blotting (WB) 14 Antibodies 172 Antibodies 29 Antibodies 20 Antibodies 5 Antibodies 3 Antibodies 6 Antibodies 1 Antibodies
Transfected Cell Culture (TCC) 1 Antibodies
Radioimmunoassay (RIA) 7 Antibodies 3 Antibodies 2 Antibodies 2 Antibodies
Radial Immunodiffusion (RID) 1 Antibodies
Neutralization (Neut) 21 Antibodies 6 Antibodies
Immunostaining (ISt) 1 Antibodies
Immunoprecipitation (IP) 21 Antibodies 2 Antibodies 2 Antibodies

General

Antigen Fibroblast Growth Factor 2 (Basic) (FGF2) Antibodies
Epitope Middle Region
(14), (13), (12), (12), (10), (8), (8), (6), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
(255), (54), (39), (17), (16), (8), (4), (2), (1), (1)
Host Rabbit
(175), (107), (6), (6), (1), (1)
Clonality
Polyclonal
Conjugate This FGF2 antibody is un-conjugated
(28), (16), (11), (6), (5), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(194), (167), (90), (34), (25), (21), (19), (17), (14), (11), (11), (10), (6), (5), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-FGF2 Antibody

Target Details FGF2 Application Details Handling References for anti-FGF2 Antibody (ABIN2777106) Images
Blocking Peptide Blocking peptide for this product available: ABIN976833
Sequence RLESNNYNTY RSRKYTSWYV ALKRTGQYKL GSKTGPGQKA ILFLPMSAKS
Predicted Reactivity Guinea pig : 100 %, Human : 100 %, Mouse : 100 %, Rabbit : 100 %, Rat : 100 %, Bovine : 90 %, Horse : 90 %, Pig : 90 %, Sheep : 90 %, African clawed frog : 80 %, Chicken : 80 %, Zebrafish : 80 %
Characteristics This is a rabbit polyclonal antibody against FGF2. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human FGF2

Target Details FGF2

Product Details anti-FGF2 Antibody Application Details Handling References for anti-FGF2 Antibody (ABIN2777106) Images back to top
Antigen
Alternative Name FGF2 (FGF2 Antibody Abstract)
Background The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
Molecular Weight 31 kDa
Gene ID 2247
NCBI Accession NM_002006, NP_001997
UniProt P09038
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-FGF2 Antibody Target Details FGF2 Handling References for anti-FGF2 Antibody (ABIN2777106) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 288 AA

Restrictions For Research Use only

Handling

Product Details anti-FGF2 Antibody Target Details FGF2 Application Details References for anti-FGF2 Antibody (ABIN2777106) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-FGF2 Antibody (ABIN2777106)

Product Details anti-FGF2 Antibody Target Details FGF2 Application Details Handling Images back to top
Background publications

Goodger, Robinson, Murphy, Gasiunas, Harmer, Blundell, Pye, Gallagher: "Evidence that heparin saccharides promote FGF2 mitogenesis through two distinct mechanisms." in: The Journal of biological chemistry, Vol. 283, Issue 19, pp. 13001-8, 2008

Images

Product Details anti-FGF2 Antibody Target Details FGF2 Application Details Handling References for anti-FGF2 Antibody (ABIN2777106) back to top
Supplier Images
Western Blotting (WB) image for anti-FGF2 antibody (Fibroblast Growth Factor 2 (Basic)) (Middle Region) (ABIN2777106) WB Suggested Anti-FGF2 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: ...