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|Antigen||Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor) (FLT1) Antibodies|
|Epitope||Extracellular Domain Alternatives|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This FLT1 antibody is conjugated to Biotin Alternatives|
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-FLT1 AntibodyTarget Details FLT1 Application Details Handling Images
|Specificity||The unconjugated monoclonal antibody will recognize recombinant and naturally occurring form of VEGFR-1/Flt-1.|
|Cross-Reactivity (Details)||Species reactivity (tested):Human, Mouse.|
|Purification||Protein G Chromatography|
|Immunogen||Recombinant Human soluble extracellular Flt-1 protein (D5)|
Target Details FLT1Product Details anti-FLT1 Antibody Application Details Handling Images back to top
|Alternative Name||VEGFR-1 / Flt-1 (FLT1 Antibody Abstract)|
|Background||Endothelial cells express three different vascular endothelial growth factor (VEGF) receptors, belonging to the family of receptor tyrosine kinases (RTKs). They are named VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), VEGFR-3 (Flt-4). Their expression is almost exclusively restricted to endothelial cells, but VEGFR-1 can also be found on monocytes, dendritic cells and on trophoblast cells. The flt-1 gene was first described in 1990. The receptor contains seven immunoglobulin-like extracellular domains, a single transmembrane region and an intracellular splited tyrosine kinase domain. Compared to VEGFR-2 the Flt-1 receptor has a higher affinity for VEGF but a weaker signaling activity. VEGFR-1 thus leads not to proliferation of endothelial cells, but mediates signals for differentiation. Interestingly a naturally occuring soluble variant of VEGFR-1 (sVEGFR-1) was found in HUVE supernatants in 1996, which is generated by alternative splicing of the flt-1 mRNA.Synonyms: FLT, FLT1, FRT, Fms-like tyrosine kinase 1, Tyrosine-protein kinase FRT, Tyrosine-protein kinase receptor FLT, VEGF Receptor 1, VEGFR1, Vascular endothelial growth factor receptor 1, Vascular permeability factor receptor|
|Research Area||Cytokines, Receptors|
|Pathways||RTK Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals|
Application DetailsProduct Details anti-FLT1 Antibody Target Details FLT1 Handling Images back to top
ELISA (undiluted 1.5 - 8 μg/mL). Western blotting (1 - 10 μg/mL). Immunofluorescence. Immunoprecipitation (1 - 5 μg/mL lysate or reaction volume).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-FLT1 Antibody Target Details FLT1 Application Details Images back to top
|Reconstitution||Restore with distilled sterile water to a concentration of 0.1-1.0 mg/mL.|
|Buffer||PBS, 0.02 % Sodium Azide, 50X BSA|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|