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anti-Rat (Rattus) FLT1 antibody for Immunocytochemistry

Recommended FLT1 Antibody (supplied by: Log in to see )

Antigen
Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor) (FLT1) Antibodies
  • AI323757
  • FLT
  • FLT-1
  • Flt-1
  • FLT1
  • flt1b
  • sFlt1
  • VEGFR-1
  • VEGFR1
Epitope
N-Term
32
20
13
11
9
9
8
5
5
5
5
5
5
4
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
246
75
64
12
4
2
2
2
2
2
2
1
1
1
1
1
1
1
Host
Rabbit
189
86
7
4
1
1
Clonality
Monoclonal
Conjugate
This FLT1 antibody is un-conjugated
15
10
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
172
132
55
48
37
25
21
16
12
11
11
5
2
2
1
1
1
1
Supplier
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Available images

Catalog No. ABIN258537
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010161072 ABIN1175067 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 303-524 Log in to see Polyclonal
0.0010161072 ABIN1175070 ICC IF IHC WB FITC Rabbit IgG Log in to see Polyclonal
0.0010161072 ABIN2988378 ICC IF IP WB Rabbit IgG Log in to see Polyclonal

Similar anti-FLT1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 27 Antibodies 29 Antibodies
Flow Cytometry (FACS) 1 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 4 Antibodies 4 Antibodies
Immunofluorescence (IF) 4 Antibodies 7 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 21 Antibodies 22 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 9 Antibodies 12 Antibodies
Immunoprecipitation (IP) 2 Antibodies 10 Antibodies
Western Blotting (WB) 31 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies

General

Antigen Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor) (FLT1) Antibodies
Epitope N-Term
(32), (20), (13), (11), (9), (9), (8), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(246), (75), (64), (12), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(189), (86), (7), (4), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This FLT1 antibody is un-conjugated
(15), (10), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(172), (132), (55), (48), (37), (25), (21), (16), (12), (11), (11), (5), (2), (2), (1), (1), (1), (1)
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Product Details anti-FLT1 Antibody

Target Details FLT1 Application Details Handling Images
Specificity The antibody can detect both VEGFR1 and its splice isoform sFlt1.
Purification IgG purified
Immunogen A synthetic peptide corresponding to residues in N-terminus of human VEGFR1 was used as immunogen.
Clone Y103
Isotype IgG

Target Details FLT1

Product Details anti-FLT1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name VEGFR-1 / Flt-1 (FLT1 Antibody Abstract)
Background VEGFR-1 (vascular endothelial growth factor receptor 1) is a 180-kDa membranereceptor tyrosine kinase that is the receptor for vascular endothelial growth factor(VEGF), VEGFB and PGF. VEGF is a key regulator of physiological angiogenesisduring embryogenesis, skeletal growth and reproductive functions. VEGF has also beenimplicated in pathological angiogenesis associated with tumors, intraocular neovasculardisorders and other conditions (1). VEGFR-1 has seven extracellular Ig-like domains,the second domain from the N-terminus is necessary and sufficient for high affinityVEGF binding (2). The biological effects of VEGF are mediated by VEGFR-1 andVEGFR-2 (1), and two major tyrosine phosphorylation sites at Tyr 1213 and Tyr 1242and two minor tyrosine phosphorylation sites at Tyr 1327 and Tyr 1333 exist inVEGFR-1 (3). Alternate Names: anti-Flt 1 antibody, anti-FLT antibody, anti-Flt1 antibody, anti-Fms like tyrosine kinase 1antibody, anti-Fms related tyrosine kinase 1 antibody, anti-FRT antibody, anti-Tyrosineprotein kinase FRT antibody, anti-Tyrosine protein kinase receptor FLT antibody,anti-Vascular endothelial growth factor receptor 1 Precursor antibody, anti-Vascularpermeability factor receptor antibody, anti-VEGFR 1 antibody, anti-VEGFR1 antibody.
Gene Symbol: FLT1
Gene ID 2321, 14254, 54251
Research Area Cytokines, Receptors
Pathways RTK Signaling

Application Details

Product Details anti-FLT1 Antibody Target Details FLT1 Handling Images back to top
Application Notes In Western blot this antibody detects a band at approximately 180 kDa. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24647000)
Recommended dilutions: Flow Cytometry 1:1000, Immunocytochemistry/Immunofluorescence 1:250, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Frozen 1:250, Immunohistochemistry-Paraffin 1:250, Immunoprecipitation 1:100, Western Blot 1:500-1000
Protocol Protocol specific for VEGF Receptor 1 Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Xylene1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
Restrictions For Research Use only

Handling

Product Details anti-FLT1 Antibody Target Details FLT1 Application Details Images back to top
Format Liquid
Buffer PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.

Images

Product Details anti-FLT1 Antibody Target Details FLT1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-FLT1 antibody (Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor)) (N-Term) (ABIN258537) Western blot analysis on mouse brain tissue using anti-VEGFR-1 (N-term) (cat. #110-57...
Immunohistochemistry (IHC) image for anti-FLT1 antibody (Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor)) (N-Term) (ABIN258537) Immunohistochemical analysis of paraffin-embedded skin cancer using anti-VEGFR-1 (N-t...
Immunofluorescence (IF) image for anti-FLT1 antibody (Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor)) (N-Term) (ABIN258537) Immunofluorescent staining of A431 cells using anti-VEGFR-1 ABIN258537