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anti-Mouse (Murine) FLT4 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended FLT4 Antibody (supplied by: Log in to see )

Antigen
Fms-Related Tyrosine Kinase 4 (FLT4) Antibodies
  • 6130401C07
  • AI323512
  • cb1059
  • Chy
  • Flk-1
  • Flk1
  • Flt-4
  • FLT41
  • Krd-1
  • LMPH1A
  • Ly73
  • PCL
  • sVEGFR-2
  • VEGFR-2
  • VEGFR-3
  • VEGFR2
  • Vegfr3
  • VEGFR3
Epitope
Extracellular Domain
35
14
14
13
12
9
9
6
5
3
3
3
2
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
159
75
31
2
1
Host
Rat
120
61
16
4
1
Clonality
Monoclonal
Conjugate
This FLT4 antibody is un-conjugated
13
7
6
6
4
2
2
2
2
2
2
2
2
2
2
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
114
83
55
30
27
22
20
11
10
9
4
3
3
2
1
Supplier
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Catalog No. ABIN967368
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008982679 ABIN678578 IF (p) IHC (p) WB Rabbit IgG AA 910-960 Log in to see Polyclonal 2
0.0008982679 ABIN266201 IHC (p) IHC Rabbit IgG C-Term Log in to see Polyclonal
0.0008982679 ABIN737406 IF (p) IHC (p) WB Rabbit IgG AA 920-970 Log in to see Polyclonal 1
0.0008982679 ABIN957559 IHC (fro) FACS IHC (p) IP WB Rat IgG2a, kappa Log in to see AFL4
0.0008982679 ABIN373447 IHC (p) WB Rabbit C-Term Log in to see Polyclonal
0.0008982679 ABIN1724128 IHC (fro) FACS IHC (p) IP WB Rat IgG2a kappa Log in to see AFL4
0.0008982679 ABIN1860935 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 30-326 Log in to see Polyclonal
0.0008982679 ABIN1078659 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 30-326 Log in to see Polyclonal
0.0008982679 ABIN678580 IHC (p) WB Biotin Rabbit IgG AA 910-960 Log in to see Polyclonal
0.0008982679 ABIN678587 IHC (p) WB HRP Rabbit IgG AA 910-960 Log in to see Polyclonal
0.0008982679 ABIN737408 IHC (p) WB Biotin Rabbit IgG AA 920-970 Log in to see Polyclonal
0.0008982679 ABIN737415 IHC (p) WB HRP Rabbit IgG AA 920-970 Log in to see Polyclonal
0.0008982679 ABIN258867 IHC (p) IHC WB Rat IgG2 Log in to see RM0003-5F63
0.0008982679 ABIN1734382 IHC (p) IP WB Rabbit IgG C-Term Log in to see Polyclonal
0.0008982679 ABIN2792782 IHC (fro) IP IHC (p) IHC WB Rat IgG2a, kappa Log in to see AFL4

Top referenced anti-FLT4 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-FLT4 Antibodies

Application / Reactivity Mouse (Murine)
ELISA 19 Antibodies
Flow Cytometry (FACS) 17 Antibodies
Functional Studies (Func) 3 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (IF) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 14 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 7 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 16 Antibodies
Immunoprecipitation (IP) 7 Antibodies
Western Blotting (WB) 34 Antibodies

General

Antigen Fms-Related Tyrosine Kinase 4 (FLT4) Antibodies
Epitope Extracellular Domain
(35), (14), (14), (13), (12), (9), (9), (6), (5), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(159), (75), (31), (2), (1)
Host Rat
(120), (61), (16), (4), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This FLT4 antibody is un-conjugated
(13), (7), (6), (6), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(114), (83), (55), (30), (27), (22), (20), (11), (10), (9), (4), (3), (3), (2), (1)
Pubmed 2 references available
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Product Details anti-FLT4 Antibody

Target Details FLT4 Application Details Handling References for anti-FLT4 Antibody (ABIN967368) Images
Brand BD Pharmingen™
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Please refer to us for technical protocols.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen VEGFR-3 extracellular domain
Clone AFL4
Isotype IgG2a, kappa

Target Details FLT4

Product Details anti-FLT4 Antibody Application Details Handling References for anti-FLT4 Antibody (ABIN967368) Images back to top
Antigen
Alternative Name VEGFR-3 (FLT4 Antibody Abstract)
Background The AFL4 antibody reacts with mouse VEGFR-3, an endothelial-specific receptor tyrosine kinase (RTK) related to VEGF receptors. The immunogen used was the extracellular domain of VEGFR-3, corresponding to nucleotides 45-2354 in the GeneBank L07296. VEGFR-3 is induced in all endothelial cells (EC’s) during early embryogenesis, and its expression eventually disappears from the vascular endothelial cells of adult tissues. VEGFR-3 is constitutively expressed in the adult lymphatic endothelium. Although VEGFR-3 is not expressed in adult blood vessels, it is induced in vascular endothelial cells of tumor-bearing tissues. Studies have shown that both VEGF-C and VEGF-D, ligands for VEGFR-3, promote lymphatic vessel proliferation throughout central tumor areas and at their peripheries, demonstrating that such molecules aid in the spread of tumor cells from the primary tumor to local lymph nodes.
Pathways RTK Signaling

Application Details

Product Details anti-FLT4 Antibody Target Details FLT4 Handling References for anti-FLT4 Antibody (ABIN967368) Images back to top
Application Notes Immunohistochemistry:
The AFL4 antibody, specific for mouse VEGFR-3, is tested for immunohistochemical staining of acetone-fixed frozen sections and zinc-fixed and formalin-fixed, paraffin-embedded sections. Antigen retrieval with Retrievagen A is recommended for formalin-fixed, paraffin sections. Tissues tested were mouse spleen, liver, kidney, lung and small intestine. The antibody stains endothelial cells of lymphatic vessels. The isotype control recommended for use with this antibody is purified rat IgG2akappa. For optimal indirect immunohistochemical staining, the AFL4 antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination with polyclonal, biotin conjugated anti-rat Igs (multiple adsorbed) as the secondary antibody and Streptavidin-HRP together with the DAB Detection System. More conveniently, the Anti-Rat Ig HRP Detection Kit, which contains the biotinylated secondary antibody, antibody diluent, streptavidin-HRP and DAB substrate, can be used for staining.
Restrictions For Research Use only

Handling

Product Details anti-FLT4 Antibody Target Details FLT4 Application Details References for anti-FLT4 Antibody (ABIN967368) Images back to top
Format Liquid
Buffer Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-FLT4 Antibody (ABIN967368)

Product Details anti-FLT4 Antibody Target Details FLT4 Application Details Handling Images back to top
Product cited in:

Stacker, Caesar, Baldwin, Thornton, Williams, Prevo, Jackson, Nishikawa, Kubo, Achen: "VEGF-D promotes the metastatic spread of tumor cells via the lymphatics." in: Nature medicine, Vol. 7, Issue 2, pp. 186-91, 2001

Kubo, Fujiwara, Jussila, Hashi, Ogawa, Shimizu, Awane, Sakai, Takabayashi, Alitalo, Yamaoka, Nishikawa: "Involvement of vascular endothelial growth factor receptor-3 in maintenance of integrity of endothelial cell lining during tumor angiogenesis." in: Blood, Vol. 96, Issue 2, pp. 546-53, 2000

Images

Product Details anti-FLT4 Antibody Target Details FLT4 Application Details Handling References for anti-FLT4 Antibody (ABIN967368) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-FLT4 antibody (Fms-Related Tyrosine Kinase 4) (Extracellular Domain) (ABIN967368) Immunohistochemical staining of mouse liver cells. Formalin-fixed, paraffin-embedded ...