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anti-Human HBEGF antibody for Immunoprecipitation

Recommended HBEGF Antibody (supplied by: Log in to see )

Antigen
Heparin-Binding EGF-Like Growth Factor (HBEGF) Antibodies
  • AW047313
  • Dtr
  • DTR
  • DTS
  • Dts
  • DTSF
  • GFHB
  • HB-EGF
  • Hb-egf
  • hbegf
  • HBEGF
  • HEGFL
  • Hegfl
  • zgc:165443
  • zgc:175091
Epitope
EGF Like Domain
16
13
13
11
6
6
5
4
3
2
2
1
1
1
1
1
Reactivity
Human
58
19
16
Host
Mouse
50
11
Clonality
Monoclonal
Conjugate
This HBEGF antibody is conjugated to Biotin
5
4
3
3
2
2
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
35
31
11
6
5
3
3
2
1
1
1
1
1
Supplier
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Catalog No. ABIN2451994
$ 434.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089235755 ABIN3201018 ICC IF IP ELISA WB Mouse IgG1 EGF Like Domain, Soluble, Bound Log in to see 4G10 4

Top referenced anti-HBEGF antibody for Immunoprecipitation

Similar anti-HBEGF Antibodies

Application / Reactivity Human
ELISA 30 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 1 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunofluorescence (IF) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 5 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 5 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Neutralization (Neut) 3 Antibodies
Western Blotting (WB) 35 Antibodies

General

Antigen Heparin-Binding EGF-Like Growth Factor (HBEGF) Antibodies
Epitope EGF Like Domain
(16), (13), (13), (11), (6), (6), (5), (4), (3), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human
(58), (19), (16)
Host Mouse
(50), (11)
Clonality (Clone)
Monoclonal   ( )
Conjugate This HBEGF antibody is conjugated to Biotin
(5), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
(35), (31), (11), (6), (5), (3), (3), (2), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-HBEGF Antibody

Target Details HBEGF Application Details Handling References for anti-HBEGF Antibody (ABIN2451994) Images
Cross-Reactivity (Details) Does not react with mouse.
Characteristics biotin/IgG = 7.5
Purification Purified
Sterility Sterile filtered
Immunogen Recombinant human HB-EGF ectodomain expressed in SF21 cell
Clone 4G10
Isotype IgG1

Target Details HBEGF

Product Details anti-HBEGF Antibody Application Details Handling References for anti-HBEGF Antibody (ABIN2451994) Images back to top
Antigen
Alternative Name HB-EGF (HBEGF Antibody Abstract)
Background Background: Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is synthesized as a membrane-anchored precursor that is proteolytically cleaved to release the soluble mature growth factor, HB-EGF. The former functions as juxtacrine and the latter as paracrine growth factor. Soluble HB-EGF shows several forms in Western blotting with apparent molecular weights 19~27 kDa due to heterogeneous O-glycosylation and N-terminal truncation. HB-EGF activates EGFR and ErbB4 and promotes the development in many tissues. In human ProHB-EGF is the cellular receptor for diphtheria toxin. Non-toxic mutant of diphtheria toxin, CRM197, inhibits HB-EGF function. As HB-EGF level is elevated in most ovarian cancer, CRM197 is being tested as an anticancer drug. The hybridoma clone 4G10 was established and characterized in the laboratory of Prof. E. Mekada of Osaka University, who is a leading scientist in this field.
UniProt Q99075
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-HBEGF Antibody Target Details HBEGF Handling References for anti-HBEGF Antibody (ABIN2451994) Images back to top
Application Notes 1) Western blotting: 0. 2~1 g/mL
2) Immunoprecipitation: 2 g/mL
3) Indirect immuno-fluorescence staining: 5~10 g/mL
Restrictions For Research Use only

Handling

Product Details anti-HBEGF Antibody Target Details HBEGF Application Details References for anti-HBEGF Antibody (ABIN2451994) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS, 50 % glycerol
Preservative Azide free
Storage -20 °C/-80 °C
Storage Comment -20 C (For long term storage: -80 C)

References for anti-HBEGF Antibody (ABIN2451994)

Product Details anti-HBEGF Antibody Target Details HBEGF Application Details Handling Images back to top
Product cited in:

Miyamoto, Hirata, Yamazaki, Kageyama, Hasuwa, Mizushima, Tanaka, Yagi, Sonoda, Kai, Kanoh, Nakano, Mekada: "Heparin-binding EGF-like growth factor is a promising target for ovarian cancer therapy." in: Cancer research, Vol. 64, Issue 16, pp. 5720-7, 2004

Prenzel, Zwick, Daub, Leserer, Abraham, Wallasch, Ullrich: "EGF receptor transactivation by G-protein-coupled receptors requires metalloproteinase cleavage of proHB-EGF." in: Nature, Vol. 402, Issue 6764, pp. 884-8, 2000

Iwamoto, Higashiyama, Mitamura, Taniguchi, Klagsbrun, Mekada et al.: "Heparin-binding EGF-like growth factor, which acts as the diphtheria toxin receptor, forms a complex with membrane protein DRAP27/CD9, which up-regulates functional receptors and diphtheria toxin ..." in: The EMBO journal, Vol. 13, Issue 10, pp. 2322-30, 1994

Higashiyama, Abraham, Miller, Fiddes, Klagsbrun: "A heparin-binding growth factor secreted by macrophage-like cells that is related to EGF." in: Science (New York, N.Y.), Vol. 251, Issue 4996, pp. 936-9, 1991