You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human IGF1R antibody for Flow Cytometry

Recommended IGF1R Antibody (supplied by: Log in to see )

Antigen
Insulin-Like Growth Factor 1 Receptor (IGF1R) Antibodies
  • A330103N21Rik
  • CD221
  • D930020L01
  • DKFZp469I0618
  • hyft
  • IGF-1R
  • igf-1r
  • IGF1R
  • IGFIR
  • IGFIRC
  • IGFR
  • IGFR1
  • JTK13
  • xIGF-1R
  • xigf1r
  • xIGFR
Epitope
Chain alpha
31
30
28
26
24
21
19
14
14
13
13
13
13
13
12
8
6
6
5
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
429
172
170
2
2
2
2
1
1
Host
Mouse
296
128
2
2
Clonality
Monoclonal
Conjugate
This IGF1R antibody is un-conjugated
20
19
18
16
10
9
8
8
8
8
8
8
8
8
6
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
263
107
100
88
70
57
38
29
9
8
7
4
3
3
2
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN181732
$ 467.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30226976 ABIN111141 Func FACS IHC (p) WB Mouse IgG1 Chain alpha Log in to see 1H7 4
0.30226976 ABIN652374 FACS IF IHC (p) WB Rabbit Ig AA 51-77, N-Term Log in to see Polyclonal 3
0.30226976 ABIN967487 FM FACS Mouse IgG1, kappa Log in to see 1H7 3
0.30226976 ABIN2662549 Dep FACS IHC PE Mouse IgG1 kappa Log in to see 1H7-CD221 7
0.30226976 ABIN2665602 FACS IF IHC Mouse IgG1 kappa Log in to see 1H7-CD221 6
0.30226976 ABIN2664409 FACS Mouse IgG1 kappa Log in to see 1H7-CD221 6
0.30226976 ABIN2487672 FACS PE Mouse IgG1 Log in to see
0.30226976 ABIN2487671 FACS PE Mouse IgG1 Log in to see
0.30226976 ABIN2479301 FACS IHC (p) WB Mouse IgG1 Log in to see 1H7 2
0.30226976 ABIN2487673 IHC (fp) FACS WB Mouse IgG1 Log in to see
0.30226976 ABIN1995884 FACS PE Rabbit IgG AA 1-932 Log in to see 12
0.30226976 ABIN2487674 IHC (fp) FACS WB Mouse IgG1 Log in to see
0.30226976 ABIN2487675 IHC (fp) FACS WB Mouse IgG1 Log in to see
0.30226976 ABIN1995882 FACS FITC Rabbit IgG AA 1-932 Log in to see
0.30226976 ABIN2630035 FACS Alexa Fluor 488 Mouse IgG1 kappa Log in to see 1H7
0.30226976 ABIN2678080 FACS APC Mouse IgG1 kappa Log in to see 1H7
0.30226976 ABIN2649188 FACS IF Rabbit Log in to see
0.30226976 ABIN2214106 FACS APC Rabbit Log in to see
0.0010482287 ABIN319437 FACS PE Mouse IgG1 Chain alpha Log in to see 1H7 3
0.0010482287 ABIN111142 FACS PE Mouse IgG1 Chain alpha Log in to see 1H7 2

Top referenced anti-IGF1R antibody for Flow Cytometry

Similar anti-IGF1R Antibodies

Application / Reactivity Human
Depletion (Dep) 2 Antibodies
ELISA 107 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies
Flow Cytometry (FACS) 58 Antibodies
Fluorescence Microscopy (FM) 1 Antibodies
Functional Studies (Func) 9 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 8 Antibodies
Immunofluorescence (IF) 29 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 88 Antibodies
Immunohistochemistry (IHC) 101 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 72 Antibodies
Immunoprecipitation (IP) 39 Antibodies
Intracellular Staining (ICS) 1 Antibodies
Neutralization (Neut) 3 Antibodies

General

Antigen Insulin-Like Growth Factor 1 Receptor (IGF1R) Antibodies
Epitope Chain alpha
(31), (30), (28), (26), (24), (21), (19), (14), (14), (13), (13), (13), (13), (13), (12), (8), (6), (6), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(429), (172), (170), (2), (2), (2), (2), (1), (1)
Host Mouse
(296), (128), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IGF1R antibody is un-conjugated
(20), (19), (18), (16), (10), (9), (8), (8), (8), (8), (8), (8), (8), (8), (6)
Application Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(263), (107), (100), (88), (70), (57), (38), (29), (9), (8), (7), (4), (3), (3), (2), (1), (1), (1), (1)
Pubmed 3 references available
Supplier Log in to see

Product Details anti-IGF1R Antibody

Target Details IGF1R Application Details Handling References for anti-IGF1R Antibody (ABIN181732) Images
Specificity This antibody recognises CD221. The ligands for CD221 include IGF-I and IGF-II, which bind to CD221 and activate tyrosine kinase activity, resulting in phosphorylation of several intracellular signalling proteins. Clone 1H7 is reported to partically block binding of IGF-I and IGF-II to CD221. We recommend the use of BM2226LE for this purpose.
Purification Affinity Chromatography on Protein G
Immunogen Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 myeloma cell line.
Clone 1H7
Isotype IgG1

Target Details IGF1R

Product Details anti-IGF1R Antibody Application Details Handling References for anti-IGF1R Antibody (ABIN181732) Images back to top
Antigen
Alternative Name CD221 / IGF1R (IGF1R Antibody Abstract)
Background CD221 is a 155kD receptor tyrosine kinase, also known as Insulin-like growth factor I receptor (IGF-I Receptor). CD221 is composed of two extracellular alpha-subunits and two transmembrane beta-subunits. CD221 is expressed in a range of tissues, including kidney, liver, placenta, mammary gland, brain, ovary and skin.Synonyms: IGF-I receptor, IGF1 Receptor, Insulin-like growth factor 1 receptor
Gene ID 3480
NCBI Accession NP_000866
UniProt P08069
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways RTK Signaling

Application Details

Product Details anti-IGF1R Antibody Target Details IGF1R Handling References for anti-IGF1R Antibody (ABIN181732) Images back to top
Application Notes Western Blot. Flow Cytometry: Use 10 μL of 1/10 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on Paraffin Sections (1/10-1/40): Requires protein digestionpre-treatment. Recommended Positive Control: Pancreas, placenta.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-IGF1R Antibody Target Details IGF1R Application Details References for anti-IGF1R Antibody (ABIN181732) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-IGF1R Antibody (ABIN181732)

Product Details anti-IGF1R Antibody Target Details IGF1R Application Details Handling Images back to top
Product cited in:

Hantel, Lewrick, Reincke, Süss, Beuschlein: "Liposomal doxorubicin-based treatment in a preclinical model of adrenocortical carcinoma." in: The Journal of endocrinology, Vol. 213, Issue 2, pp. 155-61, 2012

Hantel, Lewrick, Schneider, Zwermann, Perren, Reincke, Süss, Beuschlein: "Anti insulin-like growth factor I receptor immunoliposomes: a single formulation combining two anticancer treatments with enhanced therapeutic efficiency." in: The Journal of clinical endocrinology and metabolism, Vol. 95, Issue 2, pp. 943-52, 2010

Background publications

Li, Kato, Paz, Kasuya, Fujita-Yamaguchi: "Two new monoclonal antibodies against the alpha subunit of the human insulin-like growth factor-I receptor." in: Biochemical and biophysical research communications, Vol. 196, Issue 1, pp. 92-8, 1993

Images

Product Details anti-IGF1R Antibody Target Details IGF1R Application Details Handling References for anti-IGF1R Antibody (ABIN181732) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-IGF1R antibody (Insulin-Like Growth Factor 1 Receptor) (Chain alpha) (ABIN181732) Mouse anti CD221 (IGF-I Receptor alpha) 1H7