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Human MERTK ELISA Kit

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Antigen
C-Mer Proto-Oncogene Tyrosine Kinase (MERTK) ELISA Kits
  • C-EYK
  • MERTK
  • DKFZp469D2215
  • si:ch1073-268l12.2
  • MER
  • RP38
  • c-mer
  • Eyk
  • Mer
  • Nyk
  • nmf12
  • rdy
  • c-mer proto-oncogene tyrosine kinase
  • MERTK
  • LOC100219161
  • mertk
  • Mertk
Epitope
AA 26-499
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
14
6
1
Method Type
Sandwich ELISA
Detection Range
156-10000 pg/mL
Minimum Detection Limit
156 pg/mL
Application
ELISA
Options
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Catalog No. ABIN921101
$ 430.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
11.361965 ABIN419919 Sandwich ELISA Tissue Homogenate, Cell Lysate, Biological Fluids 0.31-20 ng/mL 0.31 ng/mL Log in to see
11.361965 ABIN2703325 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 0.15-40 ng/mL 0.15 ng/mL Log in to see 2
11.361965 ABIN1571194 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 0.312-20 ng/mL 0.312 ng/mL Log in to see
11.361965 ABIN2708316 Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Tissue Homogenate, Serum 156-10000 pg/mL 156 pg/mL Log in to see
11.361965 ABIN2114552 Cell Culture Supernatant, Cell Lysate, Tissue Homogenate, Serum Log in to see
1 ABIN828857 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate 62.5-4000 pg/mL 62.5 pg/mL Log in to see
1 ABIN5523516 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 0.312-20 ng/mL 0.312 ng/mL Log in to see
1 ABIN2684954 Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Serum, Tissue Homogenate 0.156-10 ng/mL 0.156 ng/mL Log in to see
1 ABIN2042590 Sandwich ELISA 6-10000 pg/mL 6 pg/mL Log in to see
1 ABIN1164614 0.312-20 ng/mL 0.312 ng/mL Log in to see
1 ABIN4971157 Sandwich ELISA Cell Culture Supernatant, Plasma, Tissue Homogenate, Serum, Cell Lysate, Biological Fluids 0.312-20 ng/mL 0.312 ng/mL Log in to see
1 ABIN2542249 Sandwich ELISA Tissue Homogenate, Cell Lysate, Biological Fluids 0.31-20 ng/mL 0.31 ng/mL Log in to see
1 ABIN2116225 0.312-20 ng/mL 0.312 ng/mL Log in to see
1 ABIN2952251 0.312-20 ng/mL 0.312 ng/mL Log in to see

Top referenced MERTK ELISA Kit for ELISA

  • Human MERTK ELISA Kit for Sandwich ELISA - ABIN2703325 (2 Publications): Qin, Wang, Ma, Yang, Fu, Liang, Huang, Yang, Zhong: The association of Tyro3/Axl/Mer signaling with inflammatory response, disease activity in patients with primary Sjögren's syndrome. in Joint, bone, spine : revue du rhumatisme 2015 (PubMed)

  • Human MERTK ELISA Kit for Sandwich ELISA - ABIN921101 (2 Publications): Graham, Dawson, Mullaney, Snodgrass, Earp: Cloning and mRNA expression analysis of a novel human protooncogene, c-mer. in Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research 1994 (PubMed)

Similar MERTK ELISA Kits

Application / Reactivity Human
ELISA 15 ELISA Kits

General

Antigen C-Mer Proto-Oncogene Tyrosine Kinase (MERTK) ELISA Kits
Epitope AA 26-499
Reactivity Human
Kits with alternative reactivity to:
(14), (6), (1)
Method Type Sandwich ELISA
Detection Range 156-10000 pg/mL
Minimum Detection Limit 156 pg/mL
Application ELISA
Pubmed 2 references available
Supplier Log in to see

Product Details MERTK ELISA Kit

Target details Application Details Handling References for MERTK Kit (ABIN921101) Images
Purpose Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Mer
Brand PicoKine™
Sample Type Cell Culture Supernatant, Cell Lysate, Tissue Homogenate, Serum
Detection Method Colorimetric
Analytical Method Quantitative
Specificity Expression system for standard: sf21
Immunogen sequence: R26-A499
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: sf21
Immunogen sequence: R26-A499

Target details

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Antigen
Alternative Name MERTK (MERTK ELISA Kit Abstract)
Background

Protein Function: Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll- like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3. .

Background: Proto-oncogene tyrosine-protein kinase MER is an enzyme that in humans is encoded by the MERTK gene. This gene is a member of the MER/AXL/TYRO3 receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type(immunoglobulin-like) domains, and one tyrosine kinase domain. Its gene is mapped to chromosome 2q14.1. Mer encodes a 984-amino acid protein with a calculated molecular mass of 109 kD. It is expressed in numerous neoplastic B- and T-cell lines. Mutations in this gene have been associated with disruption of the retinal pigment epithelium(RPE) phagocytosis pathway and onset of autosomal recessive retinitis pigmentosa(RP)

Synonyms: Tyrosine-protein kinase Mer,2.7.10.1,Proto-oncogene c-Mer,Receptor tyrosine kinase MerTK,MERTK,MER,

Full Gene Name: Tyrosine-protein kinase Mer

Cellular Localisation: Membrane, Single-pass type I membrane protein.
Gene ID 10461
UniProt Q12866
Research Area Neurology, Tyrosine Kinases
Pathways RTK Signaling

Application Details

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Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Comment

Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.

Tissue Specificity: Not expressed in normal B- and T-lymphocytes but is expressed in numerous neoplastic B- and T-cell lines. Highly expressed in testis, ovary, prostate, lung, and kidney, with lower expression in spleen, small intestine, colon, and liver.

Plate Pre-coated,Strips (12 x 8)
Protocol human Mer ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Mer has been precoated onto 96-well plates. Standards(sf21, R26-A499) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Mer is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Mer amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Mer standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, tissue lysates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Mer standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 1124, Standard deviation: 58.45, CV(%): 5.2
  • Sample 2: n=16, Mean(pg/ml): 3287, Standard deviation: 210.4, CV(%): 6.4
  • Sample 3: n=16, Mean(pg/ml): 5853, Standard deviation: 280.9, CV(%): 4.8,
  • Sample 1: n=24, Mean(pg/ml): 1374, Standard deviation: 92.1, CV(%): 6.7
  • Sample 2: n=24, Mean(pg/ml): 3467, Standard deviation: 249.6, CV(%): 7.2
  • Sample 3: n=24, Mean(pg/ml): 6124, Standard deviation: 306.2, CV(%): 5
Restrictions For Research Use only

Handling

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Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for MERTK Kit (ABIN921101)

Product Details MERTK ELISA Kit Target details Application Details Handling Images back to top
Background publications

Weier, Fung, Lersch: "Assignment of protooncogene MERTK (a.k.a. c-mer) to human chromosome 2q14.1 by in situ hybridization." in: Cytogenetics and cell genetics, Vol. 84, Issue 1-2, pp. 91-2, 1999

Graham, Dawson, Mullaney, Snodgrass, Earp: "Cloning and mRNA expression analysis of a novel human protooncogene, c-mer." in: Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research, Vol. 5, Issue 6, pp. 647-57, 1994

Images

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Supplier Images
ELISA image for MERTK ELISA Kit (C-Mer Proto-Oncogene Tyrosine Kinase) (ABIN921101) Human Mer PicoKine ELISA Kit standard curve