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anti-Human NFAT5 antibody for Gel Shift

Recommended NFAT5 Antibody (supplied by: Log in to see )

Antigen
Nuclear Factor of Activated T-Cells 5, Tonicity-Responsive (NFAT5) Antibodies
  • zgc:63502
  • wu:fi46f11
  • TonEBP
  • NFAT5
  • nfatz
  • orebp
  • nf-at5
  • nfatl1
  • tonebp
  • DKFZp469P1514
  • AI225870
  • B130038B15Rik
  • CAG-8
  • CAG80
  • NFATL1
  • OREBP
  • mKIAA0827
  • NF-AT5
  • NFATZ
  • TONEBP
  • Nfat
  • nuclear factor of activated T-cells 5, tonicity-responsive
  • nuclear factor of activated T cells 5
  • NFAT5
  • nfat5
  • Nfat5
Epitope
C-Term
16
7
7
5
3
3
2
2
2
2
1
1
1
Reactivity
Hamster, Human, Mouse (Murine), Rabbit, Rat (Rattus)
69
46
31
Host
Rabbit
59
12
Clonality
Polyclonal
Conjugate
This NFAT5 antibody is un-conjugated
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
Application
Chromatin Immunoprecipitation (ChIP), Gel Shift (GS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
37
30
22
12
11
5
3
Supplier
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Available images

Catalog No. ABIN267152
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Top referenced anti-NFAT5 antibody for Gel Shift

Similar anti-NFAT5 Antibodies

Application / Reactivity Rat (Rattus) Rabbit Mouse (Murine)
Chromatin Immunoprecipitation (ChIP) 1 Antibodies 1 Antibodies 1 Antibodies
ELISA 2 Antibodies
Gel Shift (GS) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 1 Antibodies 1 Antibodies
Immunofluorescence (IF) 1 Antibodies 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 3 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 7 Antibodies 1 Antibodies
Immunoprecipitation (IP) 1 Antibodies 1 Antibodies
Western Blotting (WB) 11 Antibodies 1 Antibodies

General

Antigen Nuclear Factor of Activated T-Cells 5, Tonicity-Responsive (NFAT5) Antibodies
Epitope C-Term
(16), (7), (7), (5), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Reactivity Hamster, Human, Mouse (Murine), Rabbit, Rat (Rattus)
(69), (46), (31)
Host Rabbit
(59), (12)
Clonality
Polyclonal
Conjugate This NFAT5 antibody is un-conjugated
(3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Chromatin Immunoprecipitation (ChIP), Gel Shift (GS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(37), (30), (22), (12), (11), (5), (3)
Pubmed 1 reference available
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Product Details anti-NFAT5 Antibody

Target Details NFAT5 Application Details Handling References for anti-NFAT5 Antibody (ABIN267152) Images
Cross-Reactivity (Details) Predicted cross-reactivity based on sequence identity: Mouse (94%).
Purification Immunogen affinity purified
Immunogen Synthetic peptide corresponding to residues C D(1439) L L V S L Q N Q G N N L T G S F(1455) of human NFAT5.
Isotype IgG

Target Details NFAT5

Product Details anti-NFAT5 Antibody Application Details Handling References for anti-NFAT5 Antibody (ABIN267152) Images back to top
Antigen
Alternative Name NFAT5 (NFAT5 Antibody Abstract)
Background Gene Symbol: NFAT5
Gene ID 10725
UniProt O94916
Research Area Chromatin and Nuclear Signaling, Transcription Factors, Adaptive Immunity
Pathways RTK Signaling, WNT Signaling

Application Details

Product Details anti-NFAT5 Antibody Target Details NFAT5 Handling References for anti-NFAT5 Antibody (ABIN267152) Images back to top
Application Notes Western Blot 1:1000, Chromatin Immunoprecipitation 1:10 - 1:500, Immunohistochemistry 1:20, Immunoprecipitation 3 μg, Immunohistochemistry-Paraffin 1:20, Gel Super Shift Assays 1:1 - 1:100, Immunofluorescence 1:100, Immunocytochemistry 1:1000ChIP usage was reported in scientific literature (PMID: 22266867). WB: Detects an approx. 170 kDa protein representing NFAT 5 in HEK293 cells transfected with the human NFAT 5 gene. ICC: Staining of NFAT 5 in HEK293 cells transfected with the human NFAT5 gene results in primarily cytoplasmic staining.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-NFAT5 Antibody Target Details NFAT5 Application Details References for anti-NFAT5 Antibody (ABIN267152) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS containing 1 mg/mL BSA
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze-thaw cycles.

References for anti-NFAT5 Antibody (ABIN267152)

Product Details anti-NFAT5 Antibody Target Details NFAT5 Application Details Handling Images back to top
Product cited in:

Johnson, Shapiro, Risbud: "RNA Sequencing Reveals a Role of TonEBP Transcription Factor in Regulation of Pro-inflammatory Genes in Response to Hyperosmolarity in Healthy Nucleus Pulposus Cells: A HOMEOSTATIC RESPONSE?" in: The Journal of biological chemistry, Vol. 291, Issue 52, pp. 26686-26697, 2016 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Images

Product Details anti-NFAT5 Antibody Target Details NFAT5 Application Details Handling References for anti-NFAT5 Antibody (ABIN267152) back to top
Supplier Images
Western Blotting (WB) image for anti-NFAT5 antibody (Nuclear Factor of Activated T-Cells 5, Tonicity-Responsive) (C-Term) (ABIN267152) Figure 1 shows a Western blot of human NFAT5 from transfected BHK cell lysate with AB...
Western Blotting (WB) image for anti-NFAT5 antibody (Nuclear Factor of Activated T-Cells 5, Tonicity-Responsive) (C-Term) (ABIN267152) Western Blot: NFAT5 Antibody [ABIN267152] - Analysis of human NFAT5 from transfected ...
Western Blotting (WB) image for anti-NFAT5 antibody (Nuclear Factor of Activated T-Cells 5, Tonicity-Responsive) (C-Term) (ABIN267152) Western Blot: NFAT5 Antibody [ABIN267152] - Analysis of NFAT5 was performed by loadin...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-NFAT5 antibody (Nuclear Factor of Activated T-Cells 5, Tonicity-Responsive) (C-Term) (ABIN267152) Immunohistochemistry-Paraffin: NFAT5 Antibody [ABIN267152] - Normal biopsies of depar...
Immunoprecipitation (IP) image for anti-NFAT5 antibody (Nuclear Factor of Activated T-Cells 5, Tonicity-Responsive) (C-Term) (ABIN267152) Immunoprecipitation: NFAT5 Antibody [ABIN267152] - Analysis of NFAT5 was performed on...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-NFAT5 antibody (Nuclear Factor of Activated T-Cells 5, Tonicity-Responsive) (C-Term) (ABIN267152) Immunohistochemistry-Paraffin: NFAT5 Antibody [ABIN267152] - Normal biopsies of depar...
Immunofluorescence (IF) image for anti-NFAT5 antibody (Nuclear Factor of Activated T-Cells 5, Tonicity-Responsive) (C-Term) (ABIN267152) Immunocytochemistry/Immunofluorescence: NFAT5 Antibody [ABIN267152] - Analysis of NFA...
Immunofluorescence (IF) image for anti-NFAT5 antibody (Nuclear Factor of Activated T-Cells 5, Tonicity-Responsive) (C-Term) (ABIN267152) Immunocytochemistry/Immunofluorescence: NFAT5 Antibody [ABIN267152] - Analysis of NFA...
Immunofluorescence (IF) image for anti-NFAT5 antibody (Nuclear Factor of Activated T-Cells 5, Tonicity-Responsive) (C-Term) (ABIN267152) Immunocytochemistry/Immunofluorescence: NFAT5 Antibody [ABIN267152] - Analysis of NFA...
Immunofluorescence (IF) image for anti-NFAT5 antibody (Nuclear Factor of Activated T-Cells 5, Tonicity-Responsive) (C-Term) (ABIN267152) Immunocytochemistry/Immunofluorescence: NFAT5 Antibody [ABIN267152] - Analysis of NFA...