You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human NFATC1 antibody for Flow Cytometry

Recommended NFATC1 Antibody (supplied by: Log in to see )

Antigen
Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1 (NFATC1) Antibodies
  • 2210017P03Rik
  • AI449492
  • AV076380
  • NF-ATc
  • nf-atc
  • NF-ATC
  • NF-ATc1
  • NFAT
  • NFAT2
  • nfat2
  • nfatc
  • NFATc
  • NFATC1
  • Nfatcb
  • NFATmac
  • si:ch211-198c22.2
Epitope
AA 197-304
29
27
14
13
13
7
6
6
5
5
5
3
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
146
35
29
2
1
1
1
Host
Mouse
100
45
1
Clonality
Monoclonal
Conjugate
This NFATC1 antibody is conjugated to Alexa Fluor 488
5
4
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Mass Cytometry (CyTOF)
118
52
40
13
11
8
8
7
6
3
2
2
2
2
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN2657092
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30402437 ABIN2662815 FACS PE Mouse IgG1 AA 197-304 Log in to see 7A6 7
0.0010469276 ABIN2666355 FACS Mouse IgG1 AA 197-304 Log in to see 7A6 8
0.0010469276 ABIN233537 GS ICC FACS IP WB Mouse IgG1 AA 1-654 Log in to see
0.0010469276 ABIN2657093 FACS CyTOF Alexa Fluor 488 Mouse IgG1 AA 197-304 Log in to see 7A6 7
0.0010469276 ABIN2662816 FACS PE Mouse IgG1 AA 197-304 Log in to see 7A6 7
0.0010469276 ABIN2666356 FACS Mouse IgG1 AA 197-304 Log in to see 7A6 8

Top referenced anti-NFATC1 antibody for Flow Cytometry

Similar anti-NFATC1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 120 Antibodies 18 Antibodies 14 Antibodies
Mass Cytometry (CyTOF) 2 Antibodies 2 Antibodies 2 Antibodies
Immunoprecipitation (IP) 9 Antibodies 2 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 14 Antibodies 4 Antibodies 4 Antibodies
Immunohistochemistry (IHC) 41 Antibodies 11 Antibodies 9 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies 11 Antibodies
Immunofluorescence (IF) 9 Antibodies

General

Antigen Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1 (NFATC1) Antibodies
Epitope AA 197-304
(29), (27), (14), (13), (13), (7), (6), (6), (5), (5), (5), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(146), (35), (29), (2), (1), (1), (1)
Host Mouse
(100), (45), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This NFATC1 antibody is conjugated to Alexa Fluor 488
(5), (4), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Mass Cytometry (CyTOF)
(118), (52), (40), (13), (11), (8), (8), (7), (6), (3), (2), (2), (2), (2), (1), (1)
Pubmed 7 references available
Supplier Log in to see

Product Details anti-NFATC1 Antibody

Target Details NFATC1 Application Details Handling References for anti-NFATC1 Antibody (ABIN2657092) Images
Purification The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Clone 7A6
Isotype IgG1

Target Details NFATC1

Product Details anti-NFATC1 Antibody Application Details Handling References for anti-NFATC1 Antibody (ABIN2657092) Images back to top
Antigen
Alternative Name NFATc1 (NFATC1 Antibody Abstract)
Background The product of this gene is a component of the nuclear factor of activated T cells DNA-binding transcription complex. The protein complex consists of NFAT1, NFAT2 (NFATc1 or NFATc), NFAT3, and NFAT4. All members of this family are transcription factors with a Rel homology domain and regulate gene transcription in concert with AP-1 (Jun/Fos) to orchestrate an effective immune response. NFAT proteins are predominantly expressed in cells of the immune system but are also expressed in skeletal muscle, keratinocytes and adipocytes, regulating cell differentiation programs in these cells. In resting cells, NFAT proteins are heavily phosphorylated and localized in the cytoplasm. Increased intracellular calcium concentrations activate the calcium/calmodulin-dependent serine phosphatase calcineurin, which dephosphorylates NFAT proteins, resulting in their subsequent translocation to the nucleus. Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. The product of this gene is an inducible nuclear component. It functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. Five transcript variants encoding distinct isoforms have been identified for this gene. Different isoforms of this protein may regulate inducible expression of different cytokine genes.
Research Area Signaling
Pathways RTK Signaling, WNT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-NFATC1 Antibody Target Details NFATC1 Handling References for anti-NFATC1 Antibody (ABIN2657092) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-NFATC1 Antibody Target Details NFATC1 Application Details References for anti-NFATC1 Antibody (ABIN2657092) Images back to top
Concentration 0.5 mg/mL
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C

References for anti-NFATC1 Antibody (ABIN2657092)

Product Details anti-NFATC1 Antibody Target Details NFATC1 Application Details Handling Images back to top
Background publications

Zhao, Wang, Liu, He, Jia: "NFATc1: functions in osteoclasts." in: The international journal of biochemistry & cell biology, Vol. 42, Issue 5, pp. 576-9, 2010

Brandt, Liman, Bendfeldt, Mueller, Reinke, Radbruch, Worm, Baumgrass: "Whole blood flow cytometric measurement of NFATc1 and IL-2 expression to analyze cyclosporine A-mediated effects in T cells." in: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 77, Issue 7, pp. 607-13, 2010

Serfling, Berberich-Siebelt, Avots: "NFAT in lymphocytes: a factor for all events?" in: Science's STKE : signal transduction knowledge environment, Vol. 2007, Issue 398, pp. pe42, 2007

Hogan, Chen, Nardone, Rao: "Transcriptional regulation by calcium, calcineurin, and NFAT." in: Genes & development, Vol. 17, Issue 18, pp. 2205-32, 2003

Timmerman, Healy, Ho, Chen, Goodnow, Crabtree: "Redundant expression but selective utilization of nuclear factor of activated T cells family members." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 6, pp. 2735-40, 1997

Hoey, Sun, Williamson, Xu: "Isolation of two new members of the NF-AT gene family and functional characterization of the NF-AT proteins." in: Immunity, Vol. 2, Issue 5, pp. 461-72, 1995

Northrop, Ullman, Crabtree: "Characterization of the nuclear and cytoplasmic components of the lymphoid-specific nuclear factor of activated T cells (NF-AT) complex." in: The Journal of biological chemistry, Vol. 268, Issue 4, pp. 2917-23, 1993

Images

Product Details anti-NFATC1 Antibody Target Details NFATC1 Application Details Handling References for anti-NFATC1 Antibody (ABIN2657092) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-NFATC1 antibody (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1) (AA 197-304) (Alexa Fluor 488) (ABIN2657092) anti-Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1 (NFATC1) (AA 197-304) antibody (Alexa Fluor 488)