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|Application / Reactivity||Mouse (Murine)||Human|
|Chromatin Immunoprecipitation (ChIP)||1 Antibodies||3 Antibodies|
|ELISA||5 Antibodies||35 Antibodies|
|Flow Cytometry (FACS)||6 Antibodies|
|Gel Shift (GS)||1 Antibodies|
|Immunocytochemistry (ICC)||2 Antibodies|
|Immunofluorescence (IF)||5 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||11 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||4 Antibodies|
|Immunoprecipitation (IP)||2 Antibodies|
|Mass Cytometry (CyTOF)||2 Antibodies|
|Western Blotting (WB)||16 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies|
|Antigen||Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1 (NFATC1) Antibodies|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This NFATC1 antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-NFATC1 AntibodyTarget Details NFATC1 Application Details Handling References for anti-NFATC1 Antibody (ABIN967559) Images
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
Target Details NFATC1Product Details anti-NFATC1 Antibody Application Details Handling References for anti-NFATC1 Antibody (ABIN967559) Images back to top
|Alternative Name||NF-ATc1 (NFATC1 Antibody Abstract)|
|Background||The NF-AT family of transcription factors are regulators of early immune response genes in T cells following their activation by CD40L, FasL and nterleukins including IL-2, -3, -4 and -5. NF-AT proteins function in both signal transduction and transcription control. The DNA binding, active NF-AT complex contains a cytoplasmic, Ca2+/calcineurin dependent, cyclosporin sensitive subunit, designated NF-ATc, which is present in the cytoplasm of resting cells. Upon cell activation, NF-ATc is dephosphorylated and thus activated by calcineurin, resulting in rapid translocation of NF-ATc to the nucleus. The active NF-AT complex also contains a nuclear component, NF-ATn. In vitro, NF-AT cooperates with the mitogenic transcription factor AP-1 to induce multiple cytokine genes. NF-ATc is encoded by four genes: NF-ATc1 (originally named NF-ATc), NF-ATc2 (originally named NF-ATp), NF-ATc3 (NF-AT3) and NF- ATc4 (NF-AT4). These proteins are differentially expressed in tissues, suggesting that each may activate distinct sets of genes. NF-ATc1 is expressed in lymphoid organs and is upregulated in activated T cell and thymocytes. NF-ATc1 is required for the development of T cells and has been shown to contribute to the production of IL-2 and the development of Th2 responses. 7A6 recognizes human and mouse NF-ATc1. The antibody recognizes a region between amino acids 197 to 304 of human NF-ATc1, which lies outside the region of homology between NF-ATc1 and NF-ATc2.3 Please note that in the original publication for the antibody (Northrop et al. 1994), the clone name 7A6 is not specifically identified.|
|Molecular Weight||100-120 kDa|
|Pathways||RTK Signaling, WNT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway|
Application DetailsProduct Details anti-NFATC1 Antibody Target Details NFATC1 Handling References for anti-NFATC1 Antibody (ABIN967559) Images back to top
|Application Notes||7A6 may be used for western blot analysis of NF-ATc1 (1-2 µg/ml). Note that while the predicted M W of NF-ATc is ~100 kDa, the observed M W may range between 100 to 120 kDa due to post-translational modification of NF-ATc. Other applications include immunoprecipitation (2 µg/ml), mobility shift assays (EMSA) and immunofluorescence microscopy of paraformaldehyde-fixed, tissue cultured cells. Jurkat human T cells (ATCC TIB-152) are suggested as a positive control for expression of NF-ATc.|
Related Products: ABIN967389, ABIN968537
|Restrictions||For Research Use only|
HandlingProduct Details anti-NFATC1 Antibody Target Details NFATC1 Application Details References for anti-NFATC1 Antibody (ABIN967559) Images back to top
|Buffer||Aqueous buffered solution containing ≤0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store undiluted at 4°C.|
References for anti-NFATC1 Antibody (ABIN967559)Product Details anti-NFATC1 Antibody Target Details NFATC1 Application Details Handling Images back to top
|Product cited in:||
Yoshida, Nishina, Takimoto et al.: "The transcription factor NF-ATc1 regulates lymphocyte proliferation and Th2 cytokine production." in: Immunity, Vol. 8, Issue 1, pp. 115-24, 1998 (PubMed).
Timmerman, Healy, Ho et al.: "Redundant expression but selective utilization of nuclear factor of activated T cells family members." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 6, pp. 2735-40, 1997 (PubMed).
Timmerman, Clipstone, Ho et al.: "Rapid shuttling of NF-AT in discrimination of Ca2+ signals and immunosuppression." in: Nature, Vol. 383, Issue 6603, pp. 837-40, 1996 (PubMed).
Northrop, Ho, Chen et al.: "NF-AT components define a family of transcription factors targeted in T-cell activation." in: Nature, Vol. 369, Issue 6480, pp. 497-502, 1994 (PubMed).
Crabtree: "Contingent genetic regulatory events in T lymphocyte activation." in: Science (New York, N.Y.), Vol. 243, Issue 4889, pp. 355-61, 1989 (PubMed).
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