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anti-Mouse (Murine) NTRK3 antibody for Immunocytochemistry

Recommended NTRK3 Antibody (supplied by: Log in to see )

Antigen
Neurotrophic tyrosine Kinase, Receptor, Type 3 (NTRK3) Antibodies
  • AW125844
  • gp145(trkC)
  • Ntrk3_tv3
  • trkC
  • TrkC
  • TRKC
Epitope
AA 50-150, Extracellular Domain
44
15
14
12
8
6
6
6
5
4
4
4
3
3
3
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
129
57
37
4
2
2
1
1
1
1
Host
Rabbit
105
40
10
6
2
Clonality
Polyclonal
Conjugate
This NTRK3 antibody is un-conjugated
7
4
4
4
4
4
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)
130
78
70
15
8
5
1
1
Supplier
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Available images

Catalog No. ABIN409292
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010161072 ABIN409290 ICC IHC (fro) IF IHC (p) IHC WB Sheep Extracellular Domain Log in to see Polyclonal

Similar anti-NTRK3 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine) Pig (Porcine)
ELISA 6 Antibodies 25 Antibodies
Immunocytochemistry (ICC) 2 Antibodies 1 Antibodies 2 Antibodies
Immunofluorescence (IF) 2 Antibodies 1 Antibodies
Immunohistochemistry (IHC) 26 Antibodies 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 9 Antibodies 2 Antibodies
Western Blotting (WB) 37 Antibodies 3 Antibodies

General

Antigen Neurotrophic tyrosine Kinase, Receptor, Type 3 (NTRK3) Antibodies
Epitope AA 50-150, Extracellular Domain
(44), (15), (14), (12), (8), (6), (6), (6), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(129), (57), (37), (4), (2), (2), (1), (1), (1), (1)
Host Rabbit
(105), (40), (10), (6), (2)
Clonality
Polyclonal
Conjugate This NTRK3 antibody is un-conjugated
(7), (4), (4), (4), (4), (4)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)
(130), (78), (70), (15), (8), (5), (1), (1)
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Product Details anti-NTRK3 Antibody

Target Details NTRK3 Application Details Handling Images
Purification affinity purified
Immunogen A synthetic peptide made to the extracellular domain of the human TrkC protein (within residues 50-150). Immunogen has 100% homology to pig

Target Details NTRK3

Product Details anti-NTRK3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name TrkC (NTRK3 Antibody Abstract)
Background Gene Symbol: NTRK3
Gene ID 4916, 18213, 29613
UniProt Q16288
Research Area Cancer
Pathways RTK Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details anti-NTRK3 Antibody Target Details NTRK3 Handling Images back to top
Application Notes This TrkC antibody is useful for Immunohistochemistry-Paraffin and Immunocytochemistry / Immunofluorescence.In ICC/IF, membrane staining was observed in neuro2a cells. In IHC-P, staining was observed in the membrane of mouse brain and spinal cord. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:1000, Immunohistochemistry 1:200, Immunohistochemistry-Paraffin 1:200
Protocol Immunocytochemistry Protocol specific for TrkC antibody Culture cells to appropriate density in 35 mm culture dishes or 6-well plates.
1. Remove culture medium and add 10 % formalin to the dish. Fix at room temperature for 30 minutes.
. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
. To block nonspecific antibody binding incubate in 10 % normal goat serum from 1 hour to overnight at room temperature.
. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.Immunohistochemistry Protocol specific for TrkC antibody Immunohistochemistry-Paraffin Embedded SectionsAntigen Unmasking:Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.
0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes. Staining:
. Wash sections in deionized water three times for 5 minutes each.
. Wash sections in wash buffer for 5 minutes.
. Block each section with 100-400 µL blocking solution for 1 hour at room temperature.
. Remove blocking solution and add 100-400 µL diluted primary antibody. Incubate overnight at 4 C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in deionized water.
. Counterstain sections in hematoxylin.
. Wash sections in deionized water two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.
Restrictions For Research Use only

Handling

Product Details anti-NTRK3 Antibody Target Details NTRK3 Application Details Images back to top
Format Liquid
Concentration 1.26 mg/mL
Buffer PBS, 30 % glycerol, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.

Images

Product Details anti-NTRK3 Antibody Target Details NTRK3 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-NTRK3 antibody (Neurotrophic tyrosine Kinase, Receptor, Type 3) (AA 50-150) (ABIN409292) anti-Neurotrophic tyrosine Kinase, Receptor, Type 3 (NTRK3) (AA 50-150), (Extracellular Domain) antibody
Immunohistochemistry (IHC) image for anti-NTRK3 antibody (Neurotrophic tyrosine Kinase, Receptor, Type 3) (AA 50-150) (ABIN409292) anti-Neurotrophic tyrosine Kinase, Receptor, Type 3 (NTRK3) (AA 50-150), (Extracellular Domain) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-NTRK3 antibody (Neurotrophic tyrosine Kinase, Receptor, Type 3) (AA 50-150) (ABIN409292) anti-Neurotrophic tyrosine Kinase, Receptor, Type 3 (NTRK3) (AA 50-150), (Extracellular Domain) antibody (Image 3)
Immunohistochemistry (IHC) image for anti-NTRK3 antibody (Neurotrophic tyrosine Kinase, Receptor, Type 3) (AA 50-150) (ABIN409292) anti-Neurotrophic tyrosine Kinase, Receptor, Type 3 (NTRK3) (AA 50-150), (Extracellular Domain) antibody (Image 4)
Immunohistochemistry (IHC) image for anti-NTRK3 antibody (Neurotrophic tyrosine Kinase, Receptor, Type 3) (AA 50-150) (ABIN409292) anti-Neurotrophic tyrosine Kinase, Receptor, Type 3 (NTRK3) (AA 50-150), (Extracellular Domain) antibody (Image 5)
Immunohistochemistry (IHC) image for anti-NTRK3 antibody (Neurotrophic tyrosine Kinase, Receptor, Type 3) (AA 50-150) (ABIN409292) anti-Neurotrophic tyrosine Kinase, Receptor, Type 3 (NTRK3) (AA 50-150), (Extracellular Domain) antibody (Image 6)
Immunohistochemistry (IHC) image for anti-NTRK3 antibody (Neurotrophic tyrosine Kinase, Receptor, Type 3) (AA 50-150) (ABIN409292) anti-Neurotrophic tyrosine Kinase, Receptor, Type 3 (NTRK3) (AA 50-150), (Extracellular Domain) antibody (Image 7)