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anti-Human PAK4 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended PAK4 Antibody (supplied by: Log in to see )

Antigen
P21-Activated Kinase 4 (PAK4) Antibodies
  • 5730488L07Rik
  • AW555722
  • mKIAA1142
Epitope
pSer99
31
17
15
13
11
8
6
4
3
3
3
3
3
2
2
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
167
59
58
14
3
3
2
1
Host
Rabbit
143
26
2
Clonality
Polyclonal
Conjugate
This PAK4 antibody is un-conjugated
11
11
10
5
5
5
3
3
3
3
3
3
3
3
2
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
125
66
42
31
20
18
7
6
3
1
1
Supplier
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Catalog No. ABIN679073
$ 291.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.342316 ABIN214461 IHC (p) WB Rabbit Internal Region Log in to see Polyclonal
0.0008986177 ABIN360324 EIA IHC (p) WB Rabbit Ig N-Term Log in to see Polyclonal 2
0.0008986177 ABIN392437 IHC (p) WB Rabbit Ig AA 156-187, N-Term Log in to see Polyclonal
0.0008986177 ABIN303231 IHC (p) WB Rabbit Center Log in to see Polyclonal
0.0008986177 ABIN127123 IHC (p) IP WB Rabbit N-Term Log in to see Polyclonal
0.0008986177 ABIN2626536 IHC (p) Mouse IgG1 AA 1-308 Log in to see 1C7
0.0008986177 ABIN679075 IHC (p) WB Biotin Rabbit IgG pSer99 Log in to see Polyclonal
0.0008986177 ABIN679082 IHC (p) WB HRP Rabbit IgG pSer99 Log in to see Polyclonal
0.0008986177 ABIN1737831 IHC (p) WB Rabbit IgG Internal Region Log in to see Polyclonal
0.0008986177 ABIN1841460 IHC (p) WB Rabbit N-Term Log in to see Polyclonal
0.0008986177 ABIN747505 IHC (p) WB Biotin Mouse IgG Log in to see Polyclonal
0.0008986177 ABIN747512 IHC (p) WB HRP Mouse IgG Log in to see Polyclonal
0.0008986177 ABIN747520 IHC (p) WB Biotin Rabbit IgG Log in to see Polyclonal
0.0008986177 ABIN747527 IHC (p) WB HRP Rabbit IgG Log in to see Polyclonal
0.0008986177 ABIN747503 IF (p) IHC (p) WB Mouse IgG Log in to see Polyclonal
0.0008986177 ABIN747518 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal
0.0008986177 ABIN4343475 ELISA IF IHC IHC (p) WB Rabbit Center Log in to see Polyclonal
0.0008986177 ABIN2963732 FACS IHC (p) WB Rabbit AA 186-206, Internal Region Log in to see Polyclonal
0.0008986177 ABIN2766754 IHC (p) WB Rabbit Internal Region Log in to see Polyclonal

Similar anti-PAK4 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine) Human
ELISA 10 Antibodies 14 Antibodies 65 Antibodies
Immunochromatography (IC) 1 Antibodies 1 Antibodies
Immunofluorescence (IF) 3 Antibodies 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 32 Antibodies 21 Antibodies
Immunohistochemistry (IHC) 8 Antibodies 11 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 10 Antibodies 11 Antibodies
Immunoprecipitation (IP) 1 Antibodies 1 Antibodies
Western Blotting (WB) 29 Antibodies 35 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Flow Cytometry (FACS) 6 Antibodies

General

Antigen P21-Activated Kinase 4 (PAK4) Antibodies
Epitope pSer99
(31), (17), (15), (13), (11), (8), (6), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(167), (59), (58), (14), (3), (3), (2), (1)
Host Rabbit
(143), (26), (2)
Clonality
Polyclonal
Conjugate This PAK4 antibody is un-conjugated
(11), (11), (10), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (2)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(125), (66), (42), (31), (20), (18), (7), (6), (3), (1), (1)
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Product Details anti-PAK4 Antibody

Target Details PAK4 Application Details Handling Images
Specificity This phosphorylation site is homologous in the listed cross reactive species at the specified location.
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic phosphopeptide derived from human PAK4 around the phosphorylation site of Ser99
Isotype IgG

Target Details PAK4

Product Details anti-PAK4 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Pak4 (PAK4 Antibody Abstract)
Background

p21-activated kinases (PAKs) belong to the family of serine/threonine kinases involved in the control of various cellular processes, including the cell cycle, dynamics of the cytoskeleton, apoptosis, oncogenic transformation, and transcription. All PAK family members are characterized by the presence of p21-binding domain. p21-activated kinases are regulated by the small GTP-binding proteins Rac and Cdc42, and lipids, which stimulate autophosphorylation and phosphorylation of exogenous substrates. Serine (Ser-474) is the likely autophosphorylation site in the kinase domain of PAK4 in vivo. Phosphospecific directed against serine 474 detect activated PAK4 on the Golgi membrane when PAK4 is co-expressed with activated Cdc42. Current data strongly implicates PAK-4 in oncogenesis. PAK4 is frequently overexpressed in human tumor cell lines of various tissue origins. Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates the proto-oncogene RAF1 and stimulates its kinase activity. Promotes cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Phosphorylates CTNND1, probably to regulate cytoskeletal organization and cell morphology. Keeps microtubules stable through MARK2 inhibition and destabilizes the F-actin network leading to the disappearance of stress fibers and focal adhesions.

Subcellular location: Cytoplasm, Nucleus, Mitochondrion


Synonyms: p21 activated kinase 4 , p21CDKN1A activated kinase 4, PAK 4, Protein kinase related to S.cerevisiae STE20 effector for Cdc42Hs, Serine threonine kinase PAK 4, Serine/threonine protein kinase PAK 4, Serine/threonine protein kinase PAK4, KIAA1142, Serine/threonine-protein kinase PAK 7, PAK7,
Gene ID 10298
Research Area Cancer, Cell Cycle, Cytoskeleton, Microfilaments, Apoptosis/Necrosis, Kinases/Phosphatases
Pathways RTK Signaling

Application Details

Product Details anti-PAK4 Antibody Target Details PAK4 Handling Images back to top
Application Notes WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
Restrictions For Research Use only

Handling

Product Details anti-PAK4 Antibody Target Details PAK4 Application Details Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 100 μg/mL BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

Images

Product Details anti-PAK4 Antibody Target Details PAK4 Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-PAK4 antibody (P21-Activated Kinase 4) (pSer99) (ABIN679073) Formalin-fixed and paraffin embedded human prostate labeled with Anti-Phospho-PAK4(Se...