We are preparing the requested document. Please wait, this may take a while...!
|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||21 Antibodies||21 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies||2 Antibodies|
|Flow Cytometry (FACS)||1 Antibodies||1 Antibodies|
|Immunofluorescence (IF)||3 Antibodies||3 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies||11 Antibodies|
|Immunohistochemistry (IHC)||15 Antibodies||15 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||8 Antibodies||8 Antibodies|
|Immunoprecipitation (IP)||1 Antibodies||1 Antibodies|
|Western Blotting (WB)||33 Antibodies||34 Antibodies|
|Antigen||Ras Homolog Enriched in Brain (RHEB) Antibodies|
|Epitope||Internal Region Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This RHEB antibody is un-conjugated Alternatives|
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
|4 references available|
|Supplier||Log in to see|
Product Details anti-RHEB AntibodyTarget Details RHEB Application Details Handling References for anti-RHEB Antibody (ABIN1003087) Images
|Blocking Peptide||Blocking peptide for this product available: ABIN1004040|
|Purification||Affinity chromatography purified via peptide column.|
|Immunogen||Rabbit polyclol Rheb antibody was raised against a 15 amino acid peptide from the middle region of human Rheb (Genbank accession No. AAH16155).|
Target Details RHEBProduct Details anti-RHEB Antibody Application Details Handling References for anti-RHEB Antibody (ABIN1003087) Images back to top
|Alternative Name||Rheb (Ras Homolog Enriched in Brain) (RHEB Antibody Abstract)|
|Background||Rheb (Ras homolog enriched in brain) is an evolutionarily conserved member of the Ras family of small GTP-binding proteins originally found to be rapidly induced by synaptic activity in the hippocampus following seizure. While it is expressed at relatively high levels in the brain, Rheb is widely expressed in other tissues and may be induced by growth factor stimulation. Similar to other family members, Rheb triggers activation of the Raf-MEK-MAPK pathway. Biochemical and genetic studies demon-strate that Rheb has an important role in regulating the insulin/Target of rapamycin (TOR) signaling pathway. TOR is a serine/threonine protein kinase that acts as a sensor for ATP and amino acids, balancing the availability of nutrients with protein translation and cell growth. A dimeric protein complex termed TSC1/TSC2 indirectly inhibits TOR activity by inhibiting Rheb via the GAP activity of TSC2.|
Application DetailsProduct Details anti-RHEB Antibody Target Details RHEB Handling References for anti-RHEB Antibody (ABIN1003087) Images back to top
|Application Notes||Rheb antibody can be used for the detection of Rheb by Western blot at 1 µg/ml. (Optimal dilution should be determined by user.) Antibody can also be used for immunohistochemistry and ELISA and might be suited for other applications not tested so far.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-RHEB Antibody Target Details RHEB Application Details References for anti-RHEB Antibody (ABIN1003087) Images back to top
|Buffer||Antibody is supplied in PBS containing 0.02% sodium azide.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.|
|Expiry Date||12 months|
References for anti-RHEB Antibody (ABIN1003087)Product Details anti-RHEB Antibody Target Details RHEB Application Details Handling Images back to top
|Product cited in:||
Inoki, Li, Xu et al.: "Rheb GTPase is a direct target of TSC2 GAP activity and regulates mTOR signaling." in: Genes & development, Vol. 17, Issue 15, pp. 1829-34, 2003 (PubMed).
Stocker, Radimerski, Schindelholz et al.: "Rheb is an essential regulator of S6K in controlling cell growth in Drosophila." in: Nature cell biology, Vol. 5, Issue 6, pp. 559-65, 2003 (PubMed).
Yee, Worley: "Rheb interacts with Raf-1 kinase and may function to integrate growth factor- and protein kinase A-dependent signals." in: Molecular and cellular biology, Vol. 17, Issue 2, pp. 921-33, 1997 (PubMed).
Yamagata, Sanders, Kaufmann et al.: "rheb, a growth factor- and synaptic activity-regulated gene, encodes a novel Ras-related protein." in: The Journal of biological chemistry, Vol. 269, Issue 23, pp. 16333-9, 1994 (PubMed).