anti-Mouse (Murine) RHEB antibody for ELISA

Recommended RHEB Antibody (supplied by: Log in to see )

Antigen
Ras Homolog Enriched in Brain (RHEB) Antibodies
  • RHEB
  • rheb2
  • im:7161941
  • zgc:153231
  • RHEB2
  • Ras homolog enriched in brain
  • ras homolog enriched in brain
  • RHEB
  • rheb
  • Rheb
Epitope
Internal Region
15
10
9
8
8
7
6
5
2
2
2
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
134
80
60
4
3
2
2
2
2
2
1
1
1
1
1
1
1
Host
Rabbit
87
47
Clonality
Polyclonal
Conjugate
This RHEB antibody is un-conjugated
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
110
30
26
16
13
7
4
3
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Catalog No. ABIN1003087
$ 466.62
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1003086 ELISA WB Rabbit IgG N-Term Log in to see Polyclonal 4
1 ABIN4350382 ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN4902224 ELISA WB Rabbit Log in to see Polyclonal
1 ABIN4350383 ELISA IF IHC IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN1031539 ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN2744044 IHC (p) ELISA WB Mouse IgG1, kappa AA 1-184 Log in to see 2C11
1 ABIN1091350 ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN1091351 ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN2964045 IHC (p) ELISA WB Mouse IgG1 kappa AA 1-184, full length Log in to see 2C11
1 ABIN1031063 IHC ELISA WB Rabbit Middle Region Log in to see Polyclonal
1 ABIN2959903 ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN2959902 IF IHC ELISA WB Rabbit Middle Region Log in to see Polyclonal
1 ABIN2435078 ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN2433457 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN1887872 ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN2430735 IHC ELISA Rabbit IgG Log in to see Polyclonal
1 ABIN2431049 IHC ELISA Rabbit IgG Log in to see Polyclonal
1 ABIN2424077 IHC ELISA Rabbit IgG Log in to see Polyclonal
1 ABIN2425557 IHC ELISA Rabbit IgG Log in to see Polyclonal
1 ABIN1887803 IHC ELISA WB Rabbit Middle Region Log in to see Polyclonal

General

Antigen Ras Homolog Enriched in Brain (RHEB) Antibodies
Epitope Internal Region
(15), (10), (9), (8), (8), (7), (6), (5), (2), (2), (2), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(134), (80), (60), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(87), (47)
Clonality
Polyclonal
Conjugate This RHEB antibody is un-conjugated
(4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(110), (30), (26), (16), (13), (7), (4), (3), (1)
Pubmed 4 references available
Supplier Log in to see

Product Details anti-RHEB Antibody

Target Details RHEB Application Details Handling References for anti-RHEB antibody (ABIN1003087) Images
Blocking Peptide Blocking peptide for this product available: ABIN1004040
Purification Affinity chromatography purified via peptide column.
Immunogen Rabbit polyclol Rheb antibody was raised against a 15 amino acid peptide from the middle region of human Rheb (Genbank accession No. AAH16155).
Isotype IgG

Target Details RHEB

Product Details anti-RHEB Antibody Application Details Handling References for anti-RHEB antibody (ABIN1003087) Images back to top
Antigen
Alternative Name Rheb (Ras Homolog Enriched in Brain) (RHEB Antibody Abstract)
Background Rheb (Ras homolog enriched in brain) is an evolutionarily conserved member of the Ras family of small GTP-binding proteins originally found to be rapidly induced by synaptic activity in the hippocampus following seizure. While it is expressed at relatively high levels in the brain, Rheb is widely expressed in other tissues and may be induced by growth factor stimulation. Similar to other family members, Rheb triggers activation of the Raf-MEK-MAPK pathway. Biochemical and genetic studies demon-strate that Rheb has an important role in regulating the insulin/Target of rapamycin (TOR) signaling pathway. TOR is a serine/threonine protein kinase that acts as a sensor for ATP and amino acids, balancing the availability of nutrients with protein translation and cell growth. A dimeric protein complex termed TSC1/TSC2 indirectly inhibits TOR activity by inhibiting Rheb via the GAP activity of TSC2.
Pathways RTK Signaling

Application Details

Product Details anti-RHEB Antibody Target Details RHEB Handling References for anti-RHEB antibody (ABIN1003087) Images back to top
Application Notes Rheb antibody can be used for the detection of Rheb by Western blot at 1 µg/ml. (Optimal dilution should be determined by user.) Antibody can also be used for immunohistochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only

Handling

Product Details anti-RHEB Antibody Target Details RHEB Application Details References for anti-RHEB antibody (ABIN1003087) Images back to top
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months

References for anti-RHEB antibody (ABIN1003087)

Product Details anti-RHEB Antibody Target Details RHEB Application Details Handling Images back to top
Product cited in:

Inoki, Li, Xu, Guan: "Rheb GTPase is a direct target of TSC2 GAP activity and regulates mTOR signaling." in: Genes & development, Vol. 17, Issue 15, pp. 1829-34, 2003

Stocker, Radimerski, Schindelholz, Wittwer, Belawat, Daram, Breuer, Thomas, Hafen: "Rheb is an essential regulator of S6K in controlling cell growth in Drosophila." in: Nature cell biology, Vol. 5, Issue 6, pp. 559-65, 2003

Yee, Worley: "Rheb interacts with Raf-1 kinase and may function to integrate growth factor- and protein kinase A-dependent signals." in: Molecular and cellular biology, Vol. 17, Issue 2, pp. 921-33, 1997

Background publications

Yamagata, Sanders, Kaufmann, Yee, Barnes, Nathans, Worley: "rheb, a growth factor- and synaptic activity-regulated gene, encodes a novel Ras-related protein." in: The Journal of biological chemistry, Vol. 269, Issue 23, pp. 16333-9, 1994