You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Chicken SHC1 antibody for Immunofluorescence

Recommended SHC1 Antibody (supplied by: Log in to see )

Antigen
SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1) Antibodies
  • p66
  • p66shc
  • P66shc
  • shc
  • SHC
  • Shc
  • SHC1
  • ShcA
  • SHCA
  • si:dkey-246i14.4
  • wu:fc10h03
Epitope
AA 359-473
41
39
17
15
13
13
13
10
9
9
9
8
7
6
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
216
170
131
9
6
5
2
2
1
1
1
Host
Mouse
203
24
Clonality
Monoclonal
Conjugate
This SHC1 antibody is un-conjugated
7
7
6
5
5
5
5
5
5
5
5
5
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
166
78
59
56
49
33
8
7
6
4
3
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN968228
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.31611317 ABIN968229 IF WB Mouse IgG1 AA 359-473 Log in to see 30-SHC 5
0.31611317 ABIN967733 IF IP WB Rabbit Ig Log in to see Polyclonal 5
0.31611317 ABIN967732 IF IP WB Rabbit Ig Log in to see Polyclonal 5

Top referenced anti-SHC1 antibody for Immunofluorescence

Similar anti-SHC1 Antibodies

Application / Reactivity Human Mouse (Murine) Dog (Canine) Rat (Rattus) Chicken
Western Blotting (WB) 160 Antibodies 113 Antibodies 10 Antibodies 79 Antibodies 6 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Immunoprecipitation (IP) 11 Antibodies 7 Antibodies 7 Antibodies 2 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 36 Antibodies 26 Antibodies 24 Antibodies
Immunohistochemistry (IHC) 81 Antibodies 49 Antibodies 46 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 7 Antibodies

General

Antigen SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1) Antibodies
Epitope AA 359-473
(41), (39), (17), (15), (13), (13), (13), (10), (9), (9), (9), (8), (7), (6), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(216), (170), (131), (9), (6), (5), (2), (2), (1), (1), (1)
Host Mouse
(203), (24)
Clonality (Clone)
Monoclonal   ( )
Conjugate This SHC1 antibody is un-conjugated
(7), (7), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (1), (1), (1)
Application Immunofluorescence (IF), Western Blotting (WB)
(166), (78), (59), (56), (49), (33), (8), (7), (6), (4), (3), (1), (1)
Pubmed 5 references available
Supplier Log in to see

Product Details anti-SHC1 Antibody

Target Details SHC1 Application Details Handling References for anti-SHC1 Antibody (ABIN968228) Images
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine), Chicken
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human SHC aa. 359-473
Clone 30-SHC
Isotype IgG1

Target Details SHC1

Product Details anti-SHC1 Antibody Application Details Handling References for anti-SHC1 Antibody (ABIN968228) Images back to top
Antigen
Alternative Name SHC (SHC1 Antibody Abstract)
Background The mammalian SHC proteins, which are expressed as multiple isoforms (46, 52, and 66 kDa), each contain a C-terminal SH2 domain and an N-terminal glycine/proline rich sequence. These proteins function as early signaling intermediates (also called adaptor proteins) which relay G protein coupled receptor (GPCR) and receptor tyrosine kinase (RTK)-induced signals via the Ras transduction pathway. To this end, the SHC proteins contain specific tyrosine residues which are phosphorylated following association with the active RTKs. Phosphorylated SHC forms a complex with the adaptor protein GRB2. Association of the SHC-GRB2 complex with the Ras guanine nucleotide exchange factor (Ras-GEF) mediates the localization of Ras-GEF to the plasma membrane. Once at the plasma membrane, Ras-GEF activates Ras by catalyzing the Ras-GTP for Ras-GDP exchange. Over-expression of SHC results in cell transformation, and phosphorylation of SHC correlates with activation of the ERK1/ERK2 kinases. The SHC proteins are mediators of signals that are essential for cell metabolism, growth, and differentiation.
Molecular Weight 66, 52 & 46 kDa
Research Area Signaling, Alzheimer's Disease, Tyrosine Kinases
Pathways RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-SHC1 Antibody Target Details SHC1 Handling References for anti-SHC1 Antibody (ABIN968228) Images back to top
Comment

Related Products: ABIN968535, ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-SHC1 Antibody Target Details SHC1 Application Details References for anti-SHC1 Antibody (ABIN968228) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.

References for anti-SHC1 Antibody (ABIN968228)

Product Details anti-SHC1 Antibody Target Details SHC1 Application Details Handling Images back to top
Product cited in:

Kiely, Sant, OConnor: "RACK1 is an insulin-like growth factor 1 (IGF-1) receptor-interacting protein that can regulate IGF-1-mediated Akt activation and protection from cell death." in: The Journal of biological chemistry, Vol. 277, Issue 25, pp. 22581-9, 2002

Ugi, Imamura, Ricketts, Olefsky: "Protein phosphatase 2A forms a molecular complex with Shc and regulates Shc tyrosine phosphorylation and downstream mitogenic signaling." in: Molecular and cellular biology, Vol. 22, Issue 7, pp. 2375-87, 2002

Imokawa, Kobayasi, Miyagishi: "Intracellular signaling mechanisms leading to synergistic effects of endothelin-1 and stem cell factor on proliferation of cultured human melanocytes. Cross-talk via trans-activation of the tyrosine kinase c-kit receptor." in: The Journal of biological chemistry, Vol. 275, Issue 43, pp. 33321-8, 2000

Laser, Willey, Jiang, Cooper, Menick, Zile, Kuppuswamy: "Integrin activation and focal complex formation in cardiac hypertrophy." in: The Journal of biological chemistry, Vol. 275, Issue 45, pp. 35624-30, 2000

Pelicci, Lanfrancone, Grignani, McGlade, Cavallo, Forni, Nicoletti, Grignani, Pawson, Pelicci: "A novel transforming protein (SHC) with an SH2 domain is implicated in mitogenic signal transduction." in: Cell, Vol. 70, Issue 1, pp. 93-104, 1992

Images

Product Details anti-SHC1 Antibody Target Details SHC1 Application Details Handling References for anti-SHC1 Antibody (ABIN968228) back to top
Supplier Images
Western Blotting (WB) image for anti-SHC1 antibody (SHC (Src Homology 2 Domain Containing) Transforming Protein 1) (AA 359-473) (ABIN968228) Western blot analysis of SHC on a HeLa cell lysate (Human cervical epitheloid carcino...
Immunofluorescence (IF) image for anti-SHC1 antibody (SHC (Src Homology 2 Domain Containing) Transforming Protein 1) (AA 359-473) (ABIN968228) Immunofluorescence staining of WI-38 cells (Human lung fibroblasts, ATCC CCL-75).