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anti-Chicken SHC1 antibody for Immunoprecipitation

Recommended SHC1 Antibody (supplied by: Log in to see )

Antigen
SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1) Antibodies
  • p66
  • p66shc
  • P66shc
  • shc
  • SHC
  • Shc
  • SHC1
  • ShcA
  • SHCA
  • si:dkey-246i14.4
  • wu:fc10h03
Reactivity
Chicken, Dog (Canine), Frog, Human, Mouse (Murine), Rat (Rattus)
216
170
131
9
6
5
2
1
1
1
1
Host
Rabbit
202
25
Clonality
Polyclonal
Conjugate
This SHC1 antibody is un-conjugated
7
7
6
5
5
5
5
5
5
5
5
5
1
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
166
78
59
56
49
33
7
7
6
4
3
1
1
Supplier
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Catalog No. ABIN967733
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089235755 ABIN967732 IF IP WB Rabbit Ig Log in to see Polyclonal 5

Top referenced anti-SHC1 antibody for Immunoprecipitation

Similar anti-SHC1 Antibodies

Application / Reactivity Human Mouse (Murine) Dog (Canine) Rat (Rattus) Chicken Frog
Western Blotting (WB) 160 Antibodies 113 Antibodies 10 Antibodies 79 Antibodies 6 Antibodies 2 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Immunoprecipitation (IP) 11 Antibodies 7 Antibodies 7 Antibodies 2 Antibodies 2 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 36 Antibodies 26 Antibodies 24 Antibodies
Immunohistochemistry (IHC) 81 Antibodies 49 Antibodies

General

Antigen SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1) Antibodies
Reactivity Chicken, Dog (Canine), Frog, Human, Mouse (Murine), Rat (Rattus)
(216), (170), (131), (9), (6), (5), (2), (1), (1), (1), (1)
Host Rabbit
(202), (25)
Clonality
Polyclonal
Conjugate This SHC1 antibody is un-conjugated
(7), (7), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (1), (1), (1)
Application Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(166), (78), (59), (56), (49), (33), (7), (7), (6), (4), (3), (1), (1)
Pubmed 5 references available
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Product Details anti-SHC1 Antibody

Target Details SHC1 Application Details Handling References for anti-SHC1 Antibody (ABIN967733) Images
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Isotype Ig

Target Details SHC1

Product Details anti-SHC1 Antibody Application Details Handling References for anti-SHC1 Antibody (ABIN967733) Images back to top
Antigen
Alternative Name SHC (SHC1 Antibody Abstract)
Background The mammalian SHC proteins, which are expressed as multiple isoforms (46, 52, and 66 kDa), each contain a C-terminal SH2 domain and an N-terminal glycine/proline rich sequence. These proteins function as early signaling intermediates (also called adaptor proteins) which relay G protein coupled receptor (GPCR) and receptor tyrosine kinase (RTK)-induced signals via the Ras transduction pathway. To this end, the SHC proteins contain specific tyrosine residues which are phosphorylated following association with the active RTKs. Phosphorylated SHC forms a complex with the adaptor protein GRB2. Association of the SHC-GRB2 complex with the Ras guanine nucleotide exchange factor (Ras-GEF) mediates the localization of Ras-GEF to the plasma membrane. Once at the plasma membrane, Ras-GEF activates Ras by catalyzing the Ras-GTP for Ras-GDP exchange. Over-expression of SHC results in cell transformation, and phosphorylation of SHC correlates with activation of the ERK1/ERK2 kinases. The SHC proteins are mediators of signals that are essential for cell metabolism, growth, and differentiation.
Molecular Weight 66/52/46 kDa
Research Area Signaling, Alzheimer's Disease, Tyrosine Kinases
Pathways RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-SHC1 Antibody Target Details SHC1 Handling References for anti-SHC1 Antibody (ABIN967733) Images back to top
Comment

Related Products: ABIN968587, ABIN967390

Restrictions For Research Use only

Handling

Product Details anti-SHC1 Antibody Target Details SHC1 Application Details References for anti-SHC1 Antibody (ABIN967733) Images back to top
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C

References for anti-SHC1 Antibody (ABIN967733)

Product Details anti-SHC1 Antibody Target Details SHC1 Application Details Handling Images back to top
Product cited in:

Zubiaur, Fernández, Ferrero, Salmerón, Malissen, Malavasi, Sancho: "CD38 is associated with lipid rafts and upon receptor stimulation leads to Akt/protein kinase B and Erk activation in the absence of the CD3-zeta immune receptor tyrosine-based activation motifs." in: The Journal of biological chemistry, Vol. 277, Issue 1, pp. 13-22, 2002

Webb, Thomas, Gonias: "Plasminogen activator inhibitor 1 functions as a urokinase response modifier at the level of cell signaling and thereby promotes MCF-7 cell growth." in: The Journal of cell biology, Vol. 152, Issue 4, pp. 741-52, 2001

Conti, Sipione, Magrassi, Bonfanti, Rigamonti, Pettirossi, Peschanski, Haddad, Pelicci, Milanesi, Pelicci, Cattaneo: "Shc signaling in differentiating neural progenitor cells." in: Nature neuroscience, Vol. 4, Issue 6, pp. 579-86, 2001

Cook, McCormick: "Inhibition by cAMP of Ras-dependent activation of Raf." in: Science (New York, N.Y.), Vol. 262, Issue 5136, pp. 1069-72, 1993

Cutler, Liu, Damen, Krystal: "Multiple cytokines induce the tyrosine phosphorylation of Shc and its association with Grb2 in hemopoietic cells." in: The Journal of biological chemistry, Vol. 268, Issue 29, pp. 21463-5, 1993

Images

Product Details anti-SHC1 Antibody Target Details SHC1 Application Details Handling References for anti-SHC1 Antibody (ABIN967733) back to top
Supplier Images
Western Blotting (WB) image for anti-SHC1 antibody (SHC (Src Homology 2 Domain Containing) Transforming Protein 1) (ABIN967733) Western blot analysis of SHC on HepG2 cell lysate. Lane 1: 1:1000, lane 2: 1:2000, la...
Immunofluorescence (IF) image for anti-SHC1 antibody (SHC (Src Homology 2 Domain Containing) Transforming Protein 1) (ABIN967733) Immunofluorescent staining of A431 cells.
 image for anti-SHC1 antibody (SHC (Src Homology 2 Domain Containing) Transforming Protein 1) (ABIN967733) anti-SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1) antibody (Image 3)