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anti-Dog (Canine) SHC1 antibody for Immunohistochemistry (Frozen Sections)

Recommended SHC1 Antibody (supplied by: Log in to see )

Antigen
SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1) Antibodies
  • SHC1
  • SHC
  • SHCA
  • P66shc
  • si:dkey-246i14.4
  • wu:fc10h03
  • shc
  • p66shc
  • Shc
  • ShcA
  • p66
  • SHC (Src homology 2 domain containing) transforming protein 1
  • Shc1p
  • squalene--hopene cyclase
  • src homology 2 domain-containing transforming protein C1
  • SHC1
  • shc1
  • LOC100229782
  • Shc1
Epitope
pTyr317
41
39
18
17
15
15
14
10
9
9
7
7
5
4
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine)
223
177
140
9
6
6
2
2
1
1
1
Host
Mouse
206
28
Clonality
Monoclonal
Conjugate
This SHC1 antibody is un-conjugated
7
7
6
5
5
5
5
5
5
5
5
5
5
5
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
161
78
66
58
44
36
11
7
5
4
4
3
1
1
Supplier
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Catalog No. ABIN126889
$ 555.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN126888 EIA IHC (fro) WB Mouse IgG1 pTyr239, pTyr240 Log in to see 1-00E-003

Similar anti-SHC1 Antibodies

Application / Reactivity Human Mouse (Murine) Dog (Canine)
Immunofluorescence (fixed cells) (IF/ICC) 3 Antibodies 2 Antibodies
Western Blotting (WB) 152 Antibodies 108 Antibodies 10 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Immunoprecipitation (IP) 11 Antibodies 8 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 36 Antibodies 26 Antibodies
Immunohistochemistry (IHC) 78 Antibodies 45 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 6 Antibodies 5 Antibodies 2 Antibodies

General

Antigen SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1) Antibodies
Epitope pTyr317
(41), (39), (18), (17), (15), (15), (14), (10), (9), (9), (7), (7), (5), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine)
(223), (177), (140), (9), (6), (6), (2), (2), (1), (1), (1)
Host Mouse
(206), (28)
Clonality (Clone)
Monoclonal   ( )
Conjugate This SHC1 antibody is un-conjugated
(7), (7), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (1), (1), (1)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(161), (78), (66), (58), (44), (36), (11), (7), (5), (4), (4), (3), (1), (1)
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Product Details anti-SHC1 Antibody

Target Details SHC1 Application Details Handling Images
Specificity This antibody specifically recognizes shc when it is phosphorylated at Tyr 317.
Characteristics Synonyms: SHC-transforming protein 1, SHC-transforming protein A, SHC-transforming protein 3,SHCA, Src homology 2 domain-containing-transforming protein C1
Purification Size exclusion chromatography.
Components incl. positive Control
Immunogen Synthetic phosphopeptide conjugated to KLH
Clone 1-50E-012
Isotype IgG1

Target Details SHC1

Product Details anti-SHC1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name SHC1 / SHC (SHC1 Antibody Abstract)
Background Mammalian cells can express three alternatively spliced isoforms of the shc adaptor protein: shc/p46, shc/p52 and shc/p66. shc/p66 contains a unique N-terminal protein domain. In addition to tyrosine phosphorylation of Tyr 239/240 and/or Tyr 317, shc/p66 is phosphorylated at serine 36, e.g. in response to EGF. Serine phosphorylation of shc/p66 impairs its ability to bind to the activated EGF receptor thus inhibiting EGF receptor downstream signalling pathways.Synonyms: SHC-transforming protein 1, SHC-transforming protein 3, SHC-transforming protein A, SHCA, Src homology 2 domain-containing-transforming protein C1
Gene ID 6464
UniProt P29353
Pathways RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, ER-Nucleus Signaling

Application Details

Product Details anti-SHC1 Antibody Target Details SHC1 Handling Images back to top
Application Notes Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-SHC1 Antibody Target Details SHC1 Application Details Images back to top
Reconstitution Restore with 1 mL H2O (15 min, RT).
Buffer 1 mL2 x PBS / 0.09 % Na-azide / PEG and Sucrose
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C/-80 °C
Storage Comment Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.
Expiry Date 12 months

Images

Product Details anti-SHC1 Antibody Target Details SHC1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-SHC1 antibody (SHC (Src Homology 2 Domain Containing) Transforming Protein 1) (pTyr317) (ABIN126889) anti-SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1) (pTyr317) antibody