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|Application / Reactivity||Human||Mouse (Murine)||Dog (Canine)|
|Immunofluorescence (fixed cells) (IF/ICC)||3 Antibodies||2 Antibodies|
|Western Blotting (WB)||152 Antibodies||108 Antibodies||10 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Immunoprecipitation (IP)||11 Antibodies||8 Antibodies||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||36 Antibodies||26 Antibodies|
|Immunohistochemistry (IHC)||78 Antibodies||45 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||6 Antibodies||5 Antibodies||2 Antibodies|
|Antigen||SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1) Antibodies|
|Reactivity||Dog (Canine), Human, Mouse (Murine) Alternatives|
|Conjugate||This SHC1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
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Product Details anti-SHC1 AntibodyTarget Details SHC1 Application Details Handling Images
|Specificity||This antibody specifically recognizes shc when it is phosphorylated at Tyr 317.|
|Characteristics||Synonyms: SHC-transforming protein 1, SHC-transforming protein A, SHC-transforming protein 3,SHCA, Src homology 2 domain-containing-transforming protein C1|
|Purification||Size exclusion chromatography.|
|Components||incl. positive Control|
|Immunogen||Synthetic phosphopeptide conjugated to KLH|
Target Details SHC1Product Details anti-SHC1 Antibody Application Details Handling Images back to top
|Alternative Name||SHC1 / SHC (SHC1 Antibody Abstract)|
|Background||Mammalian cells can express three alternatively spliced isoforms of the shc adaptor protein: shc/p46, shc/p52 and shc/p66. shc/p66 contains a unique N-terminal protein domain. In addition to tyrosine phosphorylation of Tyr 239/240 and/or Tyr 317, shc/p66 is phosphorylated at serine 36, e.g. in response to EGF. Serine phosphorylation of shc/p66 impairs its ability to bind to the activated EGF receptor thus inhibiting EGF receptor downstream signalling pathways.Synonyms: SHC-transforming protein 1, SHC-transforming protein 3, SHC-transforming protein A, SHCA, Src homology 2 domain-containing-transforming protein C1|
|Pathways||RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, ER-Nucleus Signaling|
Application DetailsProduct Details anti-SHC1 Antibody Target Details SHC1 Handling Images back to top
Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-SHC1 Antibody Target Details SHC1 Application Details Images back to top
|Reconstitution||Restore with 1 mL H2O (15 min, RT).|
|Buffer||1 mL2 x PBS / 0.09 % Na-azide / PEG and Sucrose|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||-20 °C/-80 °C|
Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.
|Expiry Date||12 months|
ImagesProduct Details anti-SHC1 Antibody Target Details SHC1 Application Details Handling back to top