This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Tie1 (rh Tie1, NP_005415.1, Met 1-Gln 760) and conjugated with PE under optimum conditions, the unreacted PE was removed.
Tyrosine-protein kinase receptor Tie-1, also known as TIE1, is a single-pass type I membrane protein which belongs to theprotein kinase superfamily, Tyr protein kinase family and Tie subfamily. TIE1 contains threeEGF-like domains, threefibronectin type-III domains, twoIg-like C2-type (immunoglobulin-like) domains and oneprotein kinase domain. TIE1 is an endothelial-cell-specific tyrosine kinase receptor. It is a cell surface protein expressed exclusively in endothelial cells. It is required for the survival and growth of microvascular endothelial cells during the capillary sprouting phase of vascular development. TIE1 is an orphan receptor tyrosine kinase that is expressed almost exclusively in endothelial cells and that is required for normal embryonic vascular development. TIE1 upregulates the cell adhesion molecules (CAMs) VCAM-1, E-selectin, and ICAM-1 through a p38-dependent mechanism. Attachment of monocyte derived immune cells to endothelial cells is also enhanced by TIE1 expression. TIE1 has a proinflammatory effect and may play a role in the endothelial inflammatory diseases such as atherosclerosis.