anti-Human TIE1 antibody for Flow Cytometry

Recommended TIE1 Antibody (supplied by: Log in to see )

Antigen
tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1) Antibodies
  • tie-1
  • JTK14
  • TIE
  • D430008P04Rik
  • tyrosine kinase with immunoglobulin-like and EGF-like domains 1
  • tyrosine-protein kinase receptor Tie-1
  • endothelium-specific receptor tyrosine kinase 1
  • TIE1
  • LOC100350418
  • CpipJ_CPIJ018933
  • tie1
  • Tie1
Epitope
AA 22-760
32
18
15
13
12
12
11
8
7
6
6
5
4
4
3
3
2
1
1
1
Reactivity
Human
232
58
29
3
1
Host
Goat
124
107
5
1
Clonality
Polyclonal
Conjugate
This TIE1 antibody is un-conjugated
13
10
10
9
6
6
5
5
5
5
5
5
5
5
5
4
4
2
2
2
1
1
1
1
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)
187
147
84
42
34
13
8
8
6
4
2
2
1
1
1
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Catalog No. ABIN4899695
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
14.052211 ABIN4896538 FACS PE Goat IgG AA 22-760 Log in to see Polyclonal
14.052211 ABIN1996996 FACS PE Mouse IgG2b AA 1-760 Log in to see 03
14.052211 ABIN449625 FACS ELISA WB Biotin Mouse IgG1 Log in to see Cl-6F12
14.052211 ABIN449624 CyTOF ELISA FACS WB Mouse IgG1 Log in to see Cl-6F12
1 ABIN155170 EIA FACS WB Biotin Mouse IgG1 Log in to see 6F12
1 ABIN155172 EIA FACS WB Mouse IgG1 Extracellular Domain Log in to see 8C9
1 ABIN155171 EIA FACS WB Mouse IgG1 Log in to see 6F12
1 ABIN1824345 FACS IP WB Mouse IgG2a Extracellular Domain Log in to see 9C1
1 ABIN5640830 FACS PE Goat IgG AA 22-760 Log in to see Polyclonal
1 ABIN5634412 CyTOF FACS IHC WB Goat IgG AA 22-760 Log in to see Polyclonal
1 ABIN933891 FACS IP WB Mouse IgG2a Extracellular Domain Log in to see 9C1
1 ABIN2574439 FACS IHC (p) WB Goat IgG AA 22-760 Log in to see Polyclonal
1 ABIN1589693 FACS ELISA WB Mouse IgG1 Log in to see 6F12
1 ABIN4247730 ELISA FACS WB Alexa Fluor 405 Mouse IgG1 Log in to see Cl-6F12
1 ABIN4247739 ELISA FACS WB DyLight 488 Mouse IgG1 Log in to see Cl-6F12
1 ABIN4247734 FACS Allophycocyanin Mouse IgG1 Log in to see Cl-6F12
1 ABIN4247732 ELISA FACS WB Alexa Fluor 647 Mouse IgG1 Log in to see Cl-6F12
1 ABIN4247741 ELISA FACS WB DyLight 755 Mouse IgG1 Log in to see Cl-6F12
1 ABIN4247736 ELISA FACS WB DyLight 650 Mouse IgG1 Log in to see Cl-6F12
1 ABIN4247738 ELISA FACS WB DyLight 680 Mouse IgG1 Log in to see Cl-6F12

General

Antigen tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1) Antibodies
Epitope AA 22-760
(32), (18), (15), (13), (12), (12), (11), (8), (7), (6), (6), (5), (4), (4), (3), (3), (2), (1), (1), (1)
Reactivity Human
(232), (58), (29), (3), (1)
Host Goat
(124), (107), (5), (1)
Clonality
Polyclonal
Conjugate This TIE1 antibody is un-conjugated
(13), (10), (10), (9), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)
(187), (147), (84), (42), (34), (13), (8), (8), (6), (4), (2), (2), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-TIE1 Antibody

Target Details TIE1 Application Details Handling References for anti-TIE1 antibody (ABIN4899695) Images
Specificity Detects human Tie-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50 % cross-reactivity with recombinant mouse Tie-1 is observed and less than 1 % cross-reactivity with recombinant human Tie-2 is observed.
Purification Antigen Affinity-purified
Sterility 0.2 μm filtered
Immunogen Mouse myeloma cell line NS0-derived recombinant human Tie-1, Ala22-Gln760, Accession # P35590
Isotype IgG

Target Details TIE1

Product Details anti-TIE1 Antibody Application Details Handling References for anti-TIE1 antibody (ABIN4899695) Images back to top
Antigen
Alternative Name Tie-1 (TIE1 Antibody Abstract)
Background Alternative Names: EC 2.7.10, EC 2.7.10.1, JTK14soluble TIE1 variant 3, receptor tyrosine kinase, soluble TIE1 variant 1, soluble TIE1 variant 2, soluble TIE1 variant 5, Tie1, TIEsoluble TIE1 variant 4, tyrosine kinase with immunoglobulin and epidermal growth factor homologydomains 1, tyrosine kinase with immunoglobulin-like and EGF-like domains 1, tyrosine-protein kinase receptor Tie-1
Gene ID 7075
UniProt P35590
Research Area Lineage Markers, Tyrosine Kinases
Pathways RTK Signaling

Application Details

Product Details anti-TIE1 Antibody Target Details TIE1 Handling References for anti-TIE1 antibody (ABIN4899695) Images back to top
Application Notes Western Blot 0.1 μg/mL
Flow Cytometry 2.5 μg/10^6 cells
Immunohistochemistry 5-15 μg/mL
CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-TIE1 Antibody Target Details TIE1 Application Details References for anti-TIE1 antibody (ABIN4899695) Images back to top
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-TIE1 antibody (ABIN4899695)

Product Details anti-TIE1 Antibody Target Details TIE1 Application Details Handling Images back to top
Product cited in:

Hakanpaa, Sipila, Leppanen, Gautam, Nurmi, Jacquemet, Eklund, Ivaska, Alitalo, Saharinen: "Endothelial destabilization by angiopoietin-2 via integrin β1 activation." in: Nature communications, Vol. 6, pp. 5962, 2015 Method employed by authors: Immunofluorescence (IF) (Sample species: Human).

Marron, Singh, Tahir, Kavumkal, Kim, Koh, Brindle: "Regulated proteolytic processing of Tie1 modulates ligand responsiveness of the receptor-tyrosine kinase Tie2." in: The Journal of biological chemistry, Vol. 282, Issue 42, pp. 30509-17, 2007 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Kim, Shin, Kim, Choi, Byun, Jeon, Suh, Kim: "Interaction between Tie receptors modulates angiogenic activity of angiopoietin2 in endothelial progenitor cells." in: Cardiovascular research, Vol. 72, Issue 3, pp. 394-402, 2006 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Danet, Pan, Luongo, Bonnet, Simon: "Expansion of human SCID-repopulating cells under hypoxic conditions." in: The Journal of clinical investigation, Vol. 112, Issue 1, pp. 126-35, 2003 Method employed by authors: Flow Cytometry (FACS) (Sample species: Human).

Marron, Hughes, Edge, Forder, Brindle: "Evidence for heterotypic interaction between the receptor tyrosine kinases TIE-1 and TIE-2." in: The Journal of biological chemistry, Vol. 275, Issue 50, pp. 39741-6, 2001 Method employed by authors: Immunoprecipitation (IP) (Sample species: Human).

Images

Product Details anti-TIE1 Antibody Target Details TIE1 Application Details Handling References for anti-TIE1 antibody (ABIN4899695) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1) (AA 22-760) antibody (ABIN4899695) Detection of Tie-1 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein...
Immunohistochemistry (IHC) image for anti-tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1) (AA 22-760) antibody (ABIN4899695) Tie-1 in Human Placenta. Tie-1 was detected in immersion fixed paraffin-embedded sect...
Flow Cytometry (FACS) image for anti-tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1) (AA 22-760) antibody (ABIN4899695) Detection of Tie-1 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein...
Immunohistochemistry (IHC) image for anti-tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1) (AA 22-760) antibody (ABIN4899695) Tie-1 in Human Placenta. Tie-1 was detected in immersion fixed paraffin-embedded sect...