TIE1 antibody (AA 435-609)

Catalog No. ABIN7425716

The Mouse Monoclonal anti-TIE1 antibody (Clone D1) (ABIN7425716) specifically detects TIE1 in IHC. The antibody is reactive with Human samples.

Quick Overview for TIE1 antibody (AA 435-609) (ABIN7425716)

Target: TIE1 (tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TIE1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: D1

Product Details anti-TIE1 Antibody

Binding Specificity: AA 435-609

Purpose: Monoclonal Antibody to Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains Protein 1 (Tie1)

Specificity: The antibody is a mouse monoclonal antibody raised against Tie1. It has been selected for its ability to recognize Tie1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Pig

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains Protein 1 (Tie1) corresdonding to Asp435~Gly609 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:500-5000
Immunohistochemistry: 5-20 μg/mL,1:50-200
Immunocytochemistry: 5-20 μg/mL,1:50-200
Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for TIE1

Target: TIE1 (tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1))

Alternative Name: Tie1

Background: JTK14, Tyrosine Kinase With Immunoglobulin And Epidermal Growth Factor Homology Domains 1, Tyrosine-protein kinase receptor Tie-1

UniProt: P35590

Pathways: RTK Signaling