anti-Human VEGF antibody for Neutralization

Recommended VEGF Antibody (supplied by: Log in to see )

Antigen
Vascular Endothelial Growth Factor A (VEGFA) Antibodies
  • vegf
  • vegfa
  • wu:fj82c06
  • VEGF
  • vegf-a
  • vpf
  • vefg
  • VEGF-A
  • VPF
  • eVEGF120
  • eVEGF164
  • MVCD1
  • Vegf
  • Vegf120
  • Vegf164
  • Vegf188
  • Vpf
  • VEGF164
  • vascular endothelial growth factor Aa
  • vascular endothelial growth factor A
  • vegfaa
  • vegfa-a
  • VEGFA
  • vegfa
  • Vegfa
Reactivity
Human
304
116
110
29
24
16
13
8
3
3
3
2
1
1
1
1
1
1
Host
Mouse
272
98
24
4
2
1
1
Clonality
Monoclonal
Conjugate
This VEGF antibody is un-conjugated
43
24
13
7
6
5
4
4
4
4
4
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Neutralization (Neut), ELISA, Western Blotting (WB)
296
196
81
58
26
26
26
18
17
17
11
10
7
5
4
2
2
2
1
1
Supplier
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Catalog No. ABIN1003427
$ 431.97
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1104978 IHC (p) Neut ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN2682752 Neut Rabbit IgG AA 1-191 Log in to see R012
1 ABIN2682750 Neut Rabbit IgG AA 1-191 Log in to see R010
1 ABIN465276 ELISA IHC Neut WB Biotin Goat Log in to see Polyclonal
1 ABIN465469 IHC Neut ELISA WB Goat Log in to see Polyclonal
1 ABIN465403 IHC Neut ELISA WB Rabbit Log in to see Polyclonal
1 ABIN1825527 Neut ELISA WB Mouse IgG1 Log in to see 2F66
1 ABIN957935 IHC (p) Neut ELISA WB Mouse IgG2b AA 27-191 Log in to see 26503
1 ABIN1589697 Neut ELISA WB Mouse IgG1 Log in to see 3(6D3)
1 ABIN199789 Neut WB Mouse IgG1, kappa Log in to see
1 ABIN2385162 Neut IHC ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN2384632 Neut ELISA WB Mouse IgG1 Log in to see 3(6D3)
1 ABIN2154531 Neut ELISA WB Mouse IgG1 Log in to see 2F66

General

Antigen Vascular Endothelial Growth Factor A (VEGFA) Antibodies
Reactivity Human
(304), (116), (110), (29), (24), (16), (13), (8), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1)
Host Mouse
(272), (98), (24), (4), (2), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This VEGF antibody is un-conjugated
(43), (24), (13), (7), (6), (5), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Neutralization (Neut), ELISA, Western Blotting (WB)
(296), (196), (81), (58), (26), (26), (26), (18), (17), (17), (11), (10), (7), (5), (4), (2), (2), (2), (1), (1)
Pubmed 2 references available
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Product Details anti-VEGF Antibody

Target Details VEGF Application Details Handling References for anti-VEGF antibody (ABIN1003427) Images
Purification Protein G affinity chromatography
Immunogen Recombint human VEGF165 protein
Clone 3
Isotype IgG1

Target Details VEGF

Product Details anti-VEGF Antibody Application Details Handling References for anti-VEGF antibody (ABIN1003427) Images back to top
Antigen
Alternative Name Vascular Endothelial Growth Factor A (VEGFA Antibody Abstract)
Background Monoclonals were produced with the help of BALB/c mice using recombinant human VEGF165 (45 kDa) as the immunizing antigen. The humanized mouse IgG1 antibody (clone #3) from hybridomas was purified from cell culture supernatant by Protein G chromatography.
Research Area Cytokines, Growth Factors, Receptors, Immunology, Inflammation, Signaling
Pathways RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling

Application Details

Product Details anti-VEGF Antibody Target Details VEGF Handling References for anti-VEGF antibody (ABIN1003427) Images back to top
Application Notes ELISA: Use at 1 – 15µg/ml. Neutralization: Block the VEGF-A induced proliferation of ACE cell at a 1:6 molar ratio of ligand to antibody. Inhibit the binding of VEGF-A to the VEGF receptors 1 (Flt-1) and 2 (KDR). Western blotting: Use at 2-10 µg/ml Optimal dilutions should be determined by each laboratory for each application.
Restrictions For Research Use only

Handling

Product Details anti-VEGF Antibody Target Details VEGF Application Details References for anti-VEGF antibody (ABIN1003427) Images back to top
Format Liquid
Buffer PBS (pH 7.4) w/o preservative
Preservative Without preservative
Handling Advice The lyophilized antibody, thought stable at room temperature, is best stored desiccated below 0°C. Reconstituted anti-VEGF-A is stable at 4°C for > one month or can be stored in working aliquots at -20°C for more than six months.
Storage -20 °C

References for anti-VEGF antibody (ABIN1003427)

Product Details anti-VEGF Antibody Target Details VEGF Application Details Handling Images back to top
Product cited in:

Kim, Li, Winer, Armanini, Gillett, Phillips, Ferrara: "Inhibition of vascular endothelial growth factor-induced angiogenesis suppresses tumour growth in vivo." in: Nature, Vol. 362, Issue 6423, pp. 841-4, 1993

Kim, Li, Houck, Winer, Ferrara: "The vascular endothelial growth factor proteins: identification of biologically relevant regions by neutralizing monoclonal antibodies." in: Growth factors (Chur, Switzerland), Vol. 7, Issue 1, pp. 53-64, 1992