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|Application / Reactivity||Rat (Rattus)||Human|
|Cell Culture (CC)||1 Antibodies||1 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies|
|Immunocytochemistry (ICC)||4 Antibodies|
|Immunofluorescence (IF)||3 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||26 Antibodies|
|Immunohistochemistry (IHC)||33 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||20 Antibodies|
|Immunoprecipitation (IP)||3 Antibodies|
|Neutralization (Neut)||2 Antibodies|
|Western Blotting (WB)||71 Antibodies|
|Antigen||Vascular Endothelial Growth Factor A (VEGFA) Antibodies|
|Epitope||AA 27-191 Alternatives|
|Reactivity||Human, Rat (Rattus) Alternatives|
|Conjugate||This VEGF antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-VEGF AntibodyTarget Details VEGF Application Details Handling Images
|Purpose||Rabbit IgG polyclonal antibody for Vascular endothelial growth factor A(VEGFA) detection. Tested with WB, IHC-P in Human,Rat.|
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Vascular endothelial growth factor A(VEGFA) detection. Tested with WB, IHC-P in Human,Rat.
Gene Name: vascular endothelial growth factor A
Protein Name: Vascular endothelial growth factor A
|Purification||Immunogen affinity purified.|
|Immunogen||E.coli-derived human VEGF recombinant protein (Position: A27-R191). Human VEGF shares 78% amino acid (aa) sequence identity with both mouse and rat VEGF.|
Target Details VEGFProduct Details anti-VEGF Antibody Application Details Handling Images back to top
|Alternative Name||VEGFA (VEGFA Antibody Abstract)|
VEGF, a homodimeric glycoprotein of relative molecular mass 45,000, is the only mitogen that specifically acts on endothelial cells. It may be a major regulator of tumor angiogenesis in vivo. It is, however, structurally related to platelet-derived growth factor. VEGF shares homology with the PDGF A chain and B chain, including conservation of all 8 cysteines found in PDGFA and PDGFB. VEGF gene contains 8 exons. VEGF induces remodeling and enhances TH2-mediated sensitization and inflammation in the lung. And this gene also can regulate haematopoietic stem cell survival by an internal autocrine loop mechanism. What's more, it also stimulates neurogenesis in vitro and in vivo.
Synonyms: MGC70609 antibody|MVCD1 antibody|Vascular endothelial growth factor A antibody|Vascular Endothelial Growth Factor antibody|Vascular Permeability Factor antibody|VEGF-A antibody|VEGFA antibody|VEGFA_HUMAN antibody|VPF antibody
|Research Area||Cancer, Growth Factors, Angiogenesis|
|Pathways||RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals|
Application DetailsProduct Details anti-VEGF Antibody Target Details VEGF Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat, Predicted Species: Human, The detection limit for VEGF is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
|Restrictions||For Research Use only|
HandlingProduct Details anti-VEGF Antibody Target Details VEGF Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
ImagesProduct Details anti-VEGF Antibody Target Details VEGF Application Details Handling back to top