anti-Human VEGFR2 antibody for Functional Studies

Recommended VEGFR2 Antibody (supplied by: Log in to see )

Antigen
Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) Antibodies
  • vegfr-2
  • KDR
  • 6130401C07
  • Flk-1
  • Flk1
  • Krd-1
  • Ly73
  • VEGFR-2
  • VEGFR2
  • sVEGFR-2
  • Vegfr-2
  • CD309
  • FLK1
  • VEGFR
  • FLK-1
  • flk-1
  • FLK/KDR
  • flk1
  • flk1b
  • kdrb
  • si:busm1-205d10.1
  • si:ch211-254j6.1
  • si:ch211-278f21.4
  • wu:fc31a09
  • fk52c05
  • flk
  • kdr
  • kdra
  • vegfr2
  • vegfr4
  • vegr2
  • wu:fk52c05
  • vascular endothelial growth factor receptor 2
  • kinase insert domain protein receptor
  • kinase insert domain receptor
  • kinase insert domain receptor (a type III receptor tyrosine kinase)
  • kinase insert domain receptor like
  • vascular endothelial growth factor receptor-2
  • vegfr-2
  • Kdr
  • KDR
  • kdr
  • kdrl
Epitope
Extracellular Domain
76
61
58
40
25
22
22
21
18
18
16
15
15
15
13
13
13
12
12
11
10
10
9
7
7
6
6
5
5
4
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
620
371
182
25
7
4
4
4
3
3
2
2
1
1
1
1
1
1
1
1
Host
Rabbit
542
142
60
4
2
2
1
Clonality
Polyclonal
Conjugate
This VEGFR2 antibody is un-conjugated
45
32
24
20
18
12
11
9
9
5
5
5
5
4
4
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
1
1
Application
Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
466
242
217
203
125
108
41
33
26
23
18
17
11
9
9
8
7
5
5
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN115682
$ 445.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN155183 EIA Func IP WB Biotin Rabbit Extracellular Domain Log in to see Polyclonal 3
1 ABIN155184 EIA Func IP WB Rabbit Extracellular Domain Log in to see Polyclonal 3
1 ABIN155185 EIA Func IHC (fro) IP WB Rabbit Extracellular Domain Log in to see Polyclonal 3
1 ABIN3024043 Func FACS IF Mouse IgG1, kappa Log in to see KDR721
1 ABIN3024044 Func FACS IF Mouse IgG1, kappa Log in to see KDR721

General

Antigen Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) Antibodies
Epitope Extracellular Domain
(76), (61), (58), (40), (25), (22), (22), (21), (18), (18), (16), (15), (15), (15), (13), (13), (13), (12), (12), (11), (10), (10), (9), (7), (7), (6), (6), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(620), (371), (182), (25), (7), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(542), (142), (60), (4), (2), (2), (1)
Clonality
Polyclonal
Conjugate This VEGFR2 antibody is un-conjugated
(45), (32), (24), (20), (18), (12), (11), (9), (9), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1)
Application Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
(466), (242), (217), (203), (125), (108), (41), (33), (26), (23), (18), (17), (11), (9), (9), (8), (7), (5), (5), (2), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-VEGFR2 Antibody

Target Details VEGFR2 Application Details Handling ProductDetails: References for anti-VEGFR2 antibody (ABIN115682) Images
Specificity The polyclonal antibody will detect native and recombinant Human VEGFR-2/KDR in ELISA experiments and on the surface or solubilized from different Human cell types.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Protein A Affinity Chromatography
Immunogen Recombinant Human soluble extracellular domain of KDR protein (D1-7). Remarks: The antibody is identical with the former described antibody r212 (see Lit.)

Target Details VEGFR2

Product Details anti-VEGFR2 Antibody Application Details Handling ProductDetails: References for anti-VEGFR2 antibody (ABIN115682) Images back to top
Antigen
Alternative Name CD309 / VEGFR-2 / Flk-1 (KDR Antibody Abstract)
Background VEGF receptor 2 is a member of a receptor tyrosine kinase family whose activation plays an essential role in a large number of biological processes such as embryonic development, wound healing, cell proliferation, migration and differentiation. Like other growth factor receptors, upon ligand binding VEGF receptor 2 dimerises and is autophosphorylated on multiple tyrosine residues. These sites can be involved in the regulation of kinase activity or serve as binding sites for SH2 and phosphotyrosine binding containing signalling proteins. Phosphorylation of Tyrosines 1054 and 1059 in the activation loop is required for activation of VEGF receptor 2 and its intrinsic tyrosine kinase activity. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.Synonyms: FLK1, KDR, Kinase NYK, Kinase insert domain receptor, Protein-tyrosine kinase receptor Flk-1, VEGF Receptor 2, VEGFR2, Vascular endothelial growth factor receptor 2
Gene ID 3791
NCBI Accession NP_002244
UniProt P35968
Research Area Cytokines, Receptors
Pathways RTK Signaling, Glycosaminoglycan Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Regulation of long-term Neuronal Synaptic Plasticity

Application Details

Product Details anti-VEGFR2 Antibody Target Details VEGFR2 Handling ProductDetails: References for anti-VEGFR2 antibody (ABIN115682) Images back to top
Application Notes ELISA: Use at 5-15 μg/mL. Western blotting: Use at 10-20 μg/mL. Immunoprecipitation: Use 1-2 μg/mg protein lysate. Neutralizing experiments: Depending on the conditions and ligand concentration startwith 25-50 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-VEGFR2 Antibody Target Details VEGFR2 Application Details ProductDetails: References for anti-VEGFR2 antibody (ABIN115682) Images back to top
Reconstitution Restore in sterile water to a concentration of 1.0 mg/mL.
Buffer PBS, pH 7.4 without preservatives or stabilizers
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment The lyophilized antibody, thought stable at room temperature, is best stored desiccated below 0 °C. Reconstituted anti-VEGFR-2/KDR is stable at 2-8 °C for one month or can be stored in working aliquots at -20 °C for more than six months.

ProductDetails: References for anti-VEGFR2 antibody (ABIN115682)

Product Details anti-VEGFR2 Antibody Target Details VEGFR2 Application Details Handling Images back to top
Background publications

Morbidelli, Birkenhaeger, Roeckl, Granger, Kaerst, Weich, Ziche: "Distinct capillary density and progression promoted by vascular endothelial growth factor-A homodimers and heterodimers." in: Angiogenesis, Vol. 1, Issue 1, pp. 117-30, 2003

Simon, Röckl, Hornig, Gröne, Theis, Weich, Fuchs, Yayon, Gröne: "Receptors of vascular endothelial growth factor/vascular permeability factor (VEGF/VPF) in fetal and adult human kidney: localization and [125I]VEGF binding sites." in: Journal of the American Society of Nephrology : JASN, Vol. 9, Issue 6, pp. 1032-44, 1998

Clauss, Weich, Breier, Knies, Röckl, Waltenberger, Risau: "The vascular endothelial growth factor receptor Flt-1 mediates biological activities. Implications for a functional role of placenta growth factor in monocyte activation and chemotaxis." in: The Journal of biological chemistry, Vol. 271, Issue 30, pp. 17629-34, 1996