anti-Human VEGFR2 antibody for Functional Studies

Recommended VEGFR2 Antibody (supplied by: Log in to see )

Antigen
Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) Antibodies
  • vegfr-2
  • KDR
  • 6130401C07
  • Flk-1
  • Flk1
  • Krd-1
  • Ly73
  • VEGFR-2
  • VEGFR2
  • sVEGFR-2
  • Vegfr-2
  • CD309
  • FLK1
  • VEGFR
  • FLK-1
  • flk-1
  • FLK/KDR
  • flk1
  • flk1b
  • kdrb
  • si:busm1-205d10.1
  • si:ch211-254j6.1
  • si:ch211-278f21.4
  • wu:fc31a09
  • fk52c05
  • flk
  • kdr
  • kdra
  • vegfr2
  • vegfr4
  • vegr2
  • wu:fk52c05
  • vascular endothelial growth factor receptor kdr-like
  • kinase insert domain protein receptor
  • kinase insert domain receptor
  • kinase insert domain receptor (a type III receptor tyrosine kinase)
  • kinase insert domain receptor like
  • LOC100136647
  • Kdr
  • KDR
  • kdr
  • kdrl
Epitope
Extracellular Domain
76
60
56
39
25
22
22
21
18
18
16
15
15
15
13
13
13
12
12
11
11
10
9
7
6
6
6
5
5
4
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
612
364
177
25
7
4
4
4
3
3
3
2
1
1
1
1
1
1
1
1
Host
Rabbit
532
144
60
4
2
2
1
Clonality
Polyclonal
Conjugate
This VEGFR2 antibody is conjugated to Biotin
44
32
25
24
18
12
11
9
9
5
5
5
5
4
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
1
1
Application
Enzyme Immunoassay (EIA), Functional Studies (Func), Immunoprecipitation (IP), Western Blotting (WB)
462
243
212
203
120
109
42
33
26
25
18
17
11
9
9
8
7
5
5
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN155183
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN155185 EIA Func IHC (fro) IP WB Rabbit Extracellular Domain Log in to see Polyclonal 3
1 ABIN115682 EIA Func IHC (fro) IP WB Rabbit Extracellular Domain Log in to see Polyclonal 3
1 ABIN155184 EIA Func IP WB Rabbit Extracellular Domain Log in to see Polyclonal 3
1 ABIN3024043 Func FACS IF Mouse IgG1, kappa Log in to see KDR721
1 ABIN3024044 Func FACS IF Mouse IgG1, kappa Log in to see KDR721

General

Antigen Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) Antibodies
Epitope Extracellular Domain
(76), (60), (56), (39), (25), (22), (22), (21), (18), (18), (16), (15), (15), (15), (13), (13), (13), (12), (12), (11), (11), (10), (9), (7), (6), (6), (6), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(612), (364), (177), (25), (7), (4), (4), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(532), (144), (60), (4), (2), (2), (1)
Clonality
Polyclonal
Conjugate This VEGFR2 antibody is conjugated to Biotin
(44), (32), (25), (24), (18), (12), (11), (9), (9), (5), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1)
Application Enzyme Immunoassay (EIA), Functional Studies (Func), Immunoprecipitation (IP), Western Blotting (WB)
(462), (243), (212), (203), (120), (109), (42), (33), (26), (25), (18), (17), (11), (9), (9), (8), (7), (5), (5), (2), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-VEGFR2 Antibody

Target Details VEGFR2 Application Details Handling References for anti-VEGFR2 antibody (ABIN155183) Images
Specificity The antibody will detect native and recombinant VEGFR-2/KDR.
Purification Antigenaffinity chromatography with immobilised recombinant soluble VEGFR-2/KDR
Immunogen Highly pure recombinant human soluble extracellular domain of KDR (110 kDa)

Target Details VEGFR2

Product Details anti-VEGFR2 Antibody Application Details Handling References for anti-VEGFR2 antibody (ABIN155183) Images back to top
Antigen
Alternative Name CD309 / VEGFR-2 / Flk-1 (KDR Antibody Abstract)
Background VEGF receptor 2 is a member of a receptor tyrosine kinase family whose activation plays an essential role in a large number of biological processes such as embryonic development, wound healing, cell proliferation, migration and differentiation. Like other growth factor receptors, upon ligand binding VEGF receptor 2 dimerises and is autophosphorylated on multiple tyrosine residues. These sites can be involved in the regulation of kinase activity or serve as binding sites for SH2 and phosphotyrosine binding containing signalling proteins. Phosphorylation of Tyrosines 1054 and 1059 in the activation loop is required for activation of VEGF receptor 2 and its intrinsic tyrosine kinase activity. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.Synonyms: FLK1, KDR, Kinase NYK, Kinase insert domain receptor, Protein-tyrosine kinase receptor Flk-1, VEGF Receptor 2, VEGFR2, Vascular endothelial growth factor receptor 2
Gene ID 3791
NCBI Accession NP_002244
UniProt P35968
Research Area Cytokines, Receptors
Pathways RTK Signaling, Glycosaminoglycan Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling

Application Details

Product Details anti-VEGFR2 Antibody Target Details VEGFR2 Handling References for anti-VEGFR2 antibody (ABIN155183) Images back to top
Application Notes ELISA: 5-15 μg/mL. Western blot. Immunoprecipitation. Neutralizing experiments.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-VEGFR2 Antibody Target Details VEGFR2 Application Details References for anti-VEGFR2 antibody (ABIN155183) Images back to top
Reconstitution Reconstitute in sterile water to a concentration of 0.1 mg/mL.
Buffer PBS pH 7.4 w/o preservative and stabilizer
Precaution of Use Do Not Add Sodium Azide
Handling Advice Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store vial at 2-8 °C prior to restoration. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

References for anti-VEGFR2 antibody (ABIN155183)

Product Details anti-VEGFR2 Antibody Target Details VEGFR2 Application Details Handling Images back to top
Background publications

Morbidelli, Birkenhaeger, Roeckl, Granger, Kaerst, Weich, Ziche: "Distinct capillary density and progression promoted by vascular endothelial growth factor-A homodimers and heterodimers." in: Angiogenesis, Vol. 1, Issue 1, pp. 117-30, 2003

Simon, Röckl, Hornig, Gröne, Theis, Weich, Fuchs, Yayon, Gröne: "Receptors of vascular endothelial growth factor/vascular permeability factor (VEGF/VPF) in fetal and adult human kidney: localization and [125I]VEGF binding sites." in: Journal of the American Society of Nephrology : JASN, Vol. 9, Issue 6, pp. 1032-44, 1998

Clauss, Weich, Breier, Knies, Röckl, Waltenberger, Risau: "The vascular endothelial growth factor receptor Flt-1 mediates biological activities. Implications for a functional role of placenta growth factor in monocyte activation and chemotaxis." in: The Journal of biological chemistry, Vol. 271, Issue 30, pp. 17629-34, 1996