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anti-Human VEGFR2 antibody for Immunofluorescence

Recommended VEGFR2 Antibody (supplied by: Log in to see )

Antigen
Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) Antibodies
  • vegfr-2
  • KDR
  • 6130401C07
  • Flk-1
  • Flk1
  • Krd-1
  • Ly73
  • VEGFR-2
  • VEGFR2
  • sVEGFR-2
  • Vegfr-2
  • CD309
  • FLK1
  • VEGFR
  • FLK-1
  • flk-1
  • FLK/KDR
  • flk1
  • flk1b
  • kdrb
  • si:busm1-205d10.1
  • si:ch211-254j6.1
  • si:ch211-278f21.4
  • wu:fc31a09
  • fk52c05
  • flk
  • kdr
  • kdra
  • vegfr2
  • vegfr4
  • vegr2
  • wu:fk52c05
  • vascular endothelial growth factor receptor 2
  • kinase insert domain protein receptor
  • kinase insert domain receptor
  • kinase insert domain receptor (a type III receptor tyrosine kinase)
  • kinase insert domain receptor like
  • vascular endothelial growth factor receptor-2
  • vegfr-2
  • Kdr
  • KDR
  • kdr
  • kdrl
Epitope
AA 1300-1367, C-Term, Native Form
86
53
51
39
24
24
24
18
18
18
17
17
14
14
14
13
12
11
10
10
8
6
6
6
6
5
5
5
4
4
4
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Hamster, Human
580
305
155
18
5
4
3
2
2
2
2
1
1
1
1
1
1
1
1
Host
Rabbit
510
127
51
4
2
2
1
Clonality
Polyclonal
Conjugate
This VEGFR2 antibody is un-conjugated
42
27
22
16
15
12
9
7
6
5
4
4
3
3
3
3
3
3
3
3
3
3
2
2
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
434
241
214
156
116
86
45
30
26
22
18
16
9
8
7
6
5
3
2
2
1
1
1
1
1
1
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Catalog No. ABIN250774
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.418354 ABIN1532661 IF IHC ELISA Rabbit IgG Log in to see Polyclonal 1
11.418354 ABIN1106249 EIA FACS IF WB Mouse IgG1 Log in to see 4B4 3
11.418354 ABIN151962 ICC IF WB Rabbit C-Term, AA 1300-1367 Log in to see Polyclonal
11.418354 ABIN1870315 IF IHC WB Rabbit IgG pTyr1214 Log in to see Polyclonal
11.418354 ABIN1870314 IF IHC WB Rabbit IgG pTyr1175 Log in to see Polyclonal
11.418354 ABIN2705327 IC IF IHC WB Rabbit C-Term, pTyr1175 Log in to see Polyclonal
11.418354 ABIN2957790 IC IF IHC WB Rabbit C-Term, pTyr1214 Log in to see Polyclonal
11.418354 ABIN257630 ICC IF IHC (p) IHC WB Rabbit pTyr951 Log in to see Polyclonal
11.418354 ABIN151961 ICC IF WB Rabbit Internal Region, AA 1200-1300 Log in to see Polyclonal
11.418354 ABIN2501113 IF IHC Rabbit IgG Tyr1175 Log in to see Polyclonal
11.418354 ABIN2501114 IF IHC Rabbit IgG Tyr1214 Log in to see Polyclonal
11.418354 ABIN2501115 IF IHC Rabbit IgG pTyr1175 Log in to see Polyclonal
11.418354 ABIN2490518 FACS IF ELISA WB Mouse IgG1 AA 20-764 Log in to see 4B4
11.418354 ABIN2491097 IF IHC ELISA Rabbit IgG Internal Region Log in to see Polyclonal
11.418354 ABIN2493822 FACS IF WB Rabbit Ig Fraction C-Term Log in to see Polyclonal
11.418354 ABIN3028781 FACS IF IHC ELISA WB Rabbit Ig Fraction AA 1326-1356 Log in to see Polyclonal
11.418354 ABIN3020298 IF IHC WB Rabbit pTyr1175 Log in to see Polyclonal
11.418354 ABIN3020301 IF IHC WB Rabbit pTyr1214 Log in to see Polyclonal
11.418354 ABIN273374 IF IHC Rabbit IgG pTyr1175 Log in to see Polyclonal
11.418354 ABIN273375 IF IHC Rabbit IgG pTyr1175 Log in to see Polyclonal

Top referenced anti-VEGFR2 antibody for Immunofluorescence

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Application / Reactivity Human
Blocking Antibody (Inhibition) 1 Antibodies
Blocking Peptide (BP) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 3 Antibodies
Dot Blot (DB) 18 Antibodies
ELISA 201 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 16 Antibodies
Flow Cytometry (FACS) 120 Antibodies
Functional Studies (Func) 6 Antibodies
Immunochromatography (IC) 8 Antibodies
Immunocytochemistry (ICC) 42 Antibodies
Immunofluorescence (IF) 115 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 198 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 21 Antibodies

General

Antigen Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) Antibodies
Epitope AA 1300-1367, C-Term, Native Form
(86), (53), (51), (39), (24), (24), (24), (18), (18), (18), (17), (17), (14), (14), (14), (13), (12), (11), (10), (10), (8), (6), (6), (6), (6), (5), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Hamster, Human
(580), (305), (155), (18), (5), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(510), (127), (51), (4), (2), (2), (1)
Clonality
Polyclonal
Conjugate This VEGFR2 antibody is un-conjugated
(42), (27), (22), (16), (15), (12), (9), (7), (6), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(434), (241), (214), (156), (116), (86), (45), (30), (26), (22), (18), (16), (9), (8), (7), (6), (5), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 6 references available
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Product Details anti-VEGFR2 Antibody

Target Details VEGFR2 Application Details Handling References for anti-VEGFR2 Antibody (ABIN250774) Images
Specificity This has only been shown to recognize the native form (180-190 kDa protein) of VEGFR-2, known as Flk-1/ KDR/ VEGFR 2 (Vascular Endothelial Growth Factor Receptor 2), though it was raised against the precursor form.
Cross-Reactivity (Details) Predicted to react with mouse based on 100% sequence homology.
Purification Immunogen affinity purified
Immunogen A synthetic peptide made to a C-terminal region of the mouse VEGF Receptor 2 protein (between residues 1300-1367). [Swiss-Prot# P35918]

Target Details VEGFR2

Product Details anti-VEGFR2 Antibody Application Details Handling References for anti-VEGFR2 Antibody (ABIN250774) Images back to top
Antigen
Alternative Name CD309 / VEGFR-2 / Flk-1 (KDR Antibody Abstract)
Background Gene Symbol: KDR
Molecular Weight Theoretical MW: 150 kDa
Gene ID 3791
UniProt P35918
Pathways RTK Signaling

Application Details

Product Details anti-VEGFR2 Antibody Target Details VEGFR2 Handling References for anti-VEGFR2 Antibody (ABIN250774) Images back to top
Application Notes Western Blot 1 μg/mL, Immunocytochemistry/Immunofluorescence 1:10This VEGF Receptor 2 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a doublet is seen at ~150 kDa with CSF-1R/VEGFR2 chimera transfected lysates representing VEGFR-2. A non-specific band is also seen at ~85 kDa. In ICC/IF punctate membrane staining was observed in Hela cells. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Western blot protocol specific for VEGF Receptor 2 Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 %) on samples to be analyzed.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Rinse membrane with dH2O and then stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk + 1 % BSA in TBS overnight at 4 °C.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Dilute the rabbit anti-VEGFR2 primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturer's instructions (we used BioFX Super Plus ECL).Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Restrictions For Research Use only

Handling

Product Details anti-VEGFR2 Antibody Target Details VEGFR2 Application Details References for anti-VEGFR2 Antibody (ABIN250774) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Tris-Citrate/Phosphate ( pH 7.0 - 8.0)
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.

References for anti-VEGFR2 Antibody (ABIN250774)

Product Details anti-VEGFR2 Antibody Target Details VEGFR2 Application Details Handling Images back to top
Product cited in:

Lim, Yap, Lim, Goh, Ng: "Identification of autocrine growth factors secreted by CHO cells for applications in single-cell cloning media." in: Journal of proteome research, Vol. 12, Issue 7, pp. 3496-510, 2013 (Sample species: Hamster). Further details: Western Blotting

Roan, Fang, Chang, Hsu, Huang, Chiou, Tsai, Lam: "Rosuvastatin improves vascular function of arteriovenous fistula in a diabetic rat model." in: Journal of vascular surgery, Vol. 56, Issue 5, pp. 1381-9.e1, 2012 (Sample species: Rat (Rattus)). Further details: Flow Cytometry

Hu-Lowe, Zou, Grazzini, Hallin, Wickman, Amundson, Chen, Rewolinski, Yamazaki, Wu, McTigue, Murray, Kania, OConnor, Shalinsky, Bender: "Nonclinical antiangiogenesis and antitumor activities of axitinib (AG-013736), an oral, potent, and selective inhibitor of vascular endothelial growth factor receptor tyrosine kinases 1, 2, 3." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 14, Issue 22, pp. 7272-83, 2008

Takahashi, Hattori, Iwamatsu, Takizawa, Shibuya: "A novel snake venom vascular endothelial growth factor (VEGF) predominantly induces vascular permeability through preferential signaling via VEGF receptor-1." in: The Journal of biological chemistry, Vol. 279, Issue 44, pp. 46304-14, 2004

Rahimi, Dayanir, Lashkari: "Receptor chimeras indicate that the vascular endothelial growth factor receptor-1 (VEGFR-1) modulates mitogenic activity of VEGFR-2 in endothelial cells." in: The Journal of biological chemistry, Vol. 275, Issue 22, pp. 16986-92, 2000

Background publications

Gluzman-Poltorak, Cohen, Shibuya, Neufeld: "Vascular endothelial growth factor receptor-1 and neuropilin-2 form complexes." in: The Journal of biological chemistry, Vol. 276, Issue 22, pp. 18688-94, 2001

Images

Product Details anti-VEGFR2 Antibody Target Details VEGFR2 Application Details Handling References for anti-VEGFR2 Antibody (ABIN250774) back to top
Supplier Images
Western Blotting (WB) image for anti-VEGFR2 antibody (Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase)) (AA 1300-1367) (ABIN250774) Detection of VEGFR2 doublet in CSF-IR/VEGFR2 chimera transfected lysate using ABIN250...
Immunofluorescence (IF) image for anti-VEGFR2 antibody (Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase)) (AA 1300-1367) (ABIN250774) Immunocytochemistry/Immunofluorescence: VEGF R2/KDR/Flk-1 Antibody [ABIN250774] - VEG...