anti-Mouse (Murine) VEGFR2 antibody for Immunofluorescence

Recommended VEGFR2 Antibody (supplied by: Log in to see )

Antigen
Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) Antibodies
  • vegfr-2
  • KDR
  • 6130401C07
  • Flk-1
  • Flk1
  • Krd-1
  • Ly73
  • VEGFR-2
  • VEGFR2
  • sVEGFR-2
  • Vegfr-2
  • CD309
  • FLK1
  • VEGFR
  • FLK-1
  • flk-1
  • FLK/KDR
  • flk1
  • flk1b
  • kdrb
  • si:busm1-205d10.1
  • si:ch211-254j6.1
  • si:ch211-278f21.4
  • wu:fc31a09
  • fk52c05
  • flk
  • kdr
  • kdra
  • vegfr2
  • vegfr4
  • vegr2
  • wu:fk52c05
  • vascular endothelial growth factor receptor 2
  • kinase insert domain protein receptor
  • kinase insert domain receptor
  • kinase insert domain receptor (a type III receptor tyrosine kinase)
  • kinase insert domain receptor like
  • vascular endothelial growth factor receptor-2
  • vegfr-2
  • Kdr
  • KDR
  • kdr
  • kdrl
Epitope
C-Term, AA 1300-1367
75
61
58
40
25
23
22
21
18
17
16
15
15
15
13
13
13
12
12
11
10
10
9
7
7
6
6
5
5
4
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
620
370
182
25
7
4
4
4
3
3
2
2
1
1
1
1
1
1
1
1
Host
Rabbit
542
142
60
4
2
2
1
Clonality
Polyclonal
Conjugate
This VEGFR2 antibody is un-conjugated
45
32
24
20
18
12
11
9
9
5
5
5
5
4
4
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
466
242
217
203
124
108
40
34
26
24
19
17
11
9
9
8
7
6
5
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN151962
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.178167 ABIN2705327 IC IF IHC WB Rabbit C-Term, pTyr1175 Log in to see Polyclonal
11.178167 ABIN1532661 IF IHC ELISA Rabbit IgG Log in to see Polyclonal 1
11.178167 ABIN151961 ICC IF WB Rabbit Internal Region, AA 1200-1300 Log in to see Polyclonal
11.178167 ABIN2957790 IC IF IHC WB Rabbit C-Term, pTyr1214 Log in to see Polyclonal
11.178167 ABIN1870315 IF IHC WB Rabbit IgG pTyr1214 Log in to see Polyclonal
11.178167 ABIN1870314 IF IHC WB Rabbit IgG pTyr1175 Log in to see Polyclonal
11.178167 ABIN273374 IF IHC Rabbit IgG pTyr1175 Log in to see Polyclonal
11.178167 ABIN273375 IF IHC Rabbit IgG pTyr1175 Log in to see Polyclonal
11.178167 ABIN273858 IF IHC Rabbit IgG Log in to see Polyclonal
11.178167 ABIN273860 IF IHC Rabbit IgG Log in to see Polyclonal
11.178167 ABIN273859 IF IHC Rabbit IgG Log in to see Polyclonal
11.178167 ABIN273861 IF IHC Rabbit IgG Log in to see Polyclonal
1 ABIN196766 IF IHC (p) Rabbit pTyr1175 Log in to see Polyclonal 2
1 ABIN196770 IF IHC (p) WB Rabbit pTyr951 Log in to see Polyclonal 2
1 ABIN197240 IF IHC (p) WB Rabbit AA 949-953 Log in to see Polyclonal 2
1 ABIN197236 IF IHC (p) WB Rabbit Tyr1175 Log in to see Polyclonal 2
1 ABIN197238 IF IHC (p) WB Rabbit AA 1212-1216 Log in to see Polyclonal 2
1 ABIN3182010 ELISA IF IHC WB Rabbit IgG pTyr1214 Log in to see Polyclonal
1 ABIN3184634 ELISA IF IHC WB Rabbit IgG Ser696 Log in to see Polyclonal
1 ABIN3184635 ELISA IF IHC WB Rabbit IgG Ser7 Log in to see Polyclonal

General

Antigen Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) Antibodies
Epitope C-Term, AA 1300-1367
(75), (61), (58), (40), (25), (23), (22), (21), (18), (17), (16), (15), (15), (15), (13), (13), (13), (12), (12), (11), (10), (10), (9), (7), (7), (6), (6), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(620), (370), (182), (25), (7), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(542), (142), (60), (4), (2), (2), (1)
Clonality
Polyclonal
Conjugate This VEGFR2 antibody is un-conjugated
(45), (32), (24), (20), (18), (12), (11), (9), (9), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(466), (242), (217), (203), (124), (108), (40), (34), (26), (24), (19), (17), (11), (9), (9), (8), (7), (6), (5), (2), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-VEGFR2 Antibody

Target Details VEGFR2 Application Details Handling ProductDetails: References for anti-VEGFR2 antibody (ABIN151962) Images
Purification Immunogen affinity purified
Immunogen A synthetic peptide made to a C-terminal region of the mouse VEGF Receptor 2 protein (between residues 1300-1367). [Swiss-Prot# P35918]

Target Details VEGFR2

Product Details anti-VEGFR2 Antibody Application Details Handling ProductDetails: References for anti-VEGFR2 antibody (ABIN151962) Images back to top
Antigen
Alternative Name CD309 / VEGFR-2 / Flk-1 (KDR Antibody Abstract)
Background Gene Symbol: KDR
Molecular Weight Theoretical MW: 150 kDa
Gene ID 3791
UniProt P35918
Pathways RTK Signaling, Glycosaminoglycan Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling

Application Details

Product Details anti-VEGFR2 Antibody Target Details VEGFR2 Handling ProductDetails: References for anti-VEGFR2 antibody (ABIN151962) Images back to top
Application Notes Western Blot 1:250-1:1000, Immunocytochemistry/Immunofluorescence 1:50-1:500This VEGF Receptor 2 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a doublet is seen at ~150 kDa. In ICC/IF nuclear and membrane staining can be seen in V6.5 mouse embryonic stem cells (Catalog No. NBP1-41162). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Western blot Protocol for VEGF Receptor 2 Antibody
Western Blot Protocol:
1. Perform SDS-PAGE (3-8 %) on samples to be analyzed, loading 50 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk + 0.5 % BSA in TBS for 1 hour.
. Dilute the rabbit anti-VEGFR2 primary antibody in blocking buffer and incubate 2 hours at room temperature.
. Wash the membrane in water for 5 minutes and apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) and incubate 1 hour at room temperature.
. Wash the blot in TBS containing 0.05-0.1 % Tween-20 for 10-20 minutes.
. Wash the blot in type I water for an additional 10-20 minutes (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturer's instructions (Amersham's ECL is the standard reagent used).Note: Tween-20 can be added to the blocking buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.Immunocytochemistry/Immunofluorescence Protocol for VEGF Receptor 2 Antibody Immunocytochemistry ProtocolCulture cells to appropriate density in 35 mm culture dishes or 6-well plates.
. Remove culture medium and add 10 % formalin to the dish. Fix at room temperature for 30 minutes.
. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
. To block nonspecific antibody binding incubate in 10 % normal goat serum from 1 hour to overnight at room temperature.
. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.
Restrictions For Research Use only

Handling

Product Details anti-VEGFR2 Antibody Target Details VEGFR2 Application Details ProductDetails: References for anti-VEGFR2 antibody (ABIN151962) Images back to top
Format Liquid
Concentration 2.55 mg/mL
Buffer Tris-Glycine and 0.15M NaCl
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.

ProductDetails: References for anti-VEGFR2 antibody (ABIN151962)

Product Details anti-VEGFR2 Antibody Target Details VEGFR2 Application Details Handling Images back to top
Product cited in:

Motiani, Savolainen, Eskelinen, Toivanen, Ishizu, Yli-Karjanmaa, Virtanen, Parkkola, Kapanen, Grönroos, Haaparanta-Solin, Solin, Savisto, Ahotupa, Löyttyniemi, Knuuti, Nuutila, Kalliokoski et al.: "Two weeks of moderate-intensity continuous training, but not high-intensity interval training, increases insulin-stimulated intestinal glucose uptake. ..." in: Journal of applied physiology (Bethesda, Md. : 1985), Vol. 122, Issue 5, pp. 1188-1197, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Rat (Rattus)).

Images

Product Details anti-VEGFR2 Antibody Target Details VEGFR2 Application Details Handling ProductDetails: References for anti-VEGFR2 antibody (ABIN151962) back to top
Supplier Images
Western Blotting (WB) image for anti-Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) (AA 1300-1367), (C-Term) antibody (ABIN151962) Detection of VEGFR-2 doublet in CSF-1 receptor/VEGFR2 chimera transfected lysate (20 ...
Western Blotting (WB) image for anti-Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) (AA 1300-1367), (C-Term) antibody (ABIN151962) Detection of VEGFR-2 doublet in VEGFR-2 induced HUVEC lysate (50 ug) using ABIN151962...
Immunofluorescence (IF) image for anti-Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) (AA 1300-1367), (C-Term) antibody (ABIN151962) Immunocytochemistry/Immunofluorescence: VEGF R2/KDR/Flk-1 Antibody [ABIN151962] - The...