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anti-Mouse (Murine) VEGFR2 antibody for Immunoprecipitation

Recommended VEGFR2 Antibody (supplied by: Log in to see )

Antigen
Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) Antibodies
  • 6130401C07
  • CD309
  • fk52c05
  • flk
  • FLK-1
  • flk-1
  • Flk-1
  • FLK/KDR
  • Flk1
  • flk1
  • FLK1
  • flk1b
  • kdr
  • KDR
  • kdra
  • kdrb
  • Krd-1
  • Ly73
  • si:busm1-205d10.1
  • si:ch211-254j6.1
  • si:ch211-278f21.4
  • sVEGFR-2
  • VEGFR
  • Vegfr-2
  • vegfr-2
  • VEGFR-2
  • VEGFR2
  • vegfr2
  • vegfr4
  • vegr2
  • wu:fc31a09
  • wu:fk52c05
Epitope
C-Term, AA 1158-1365
79
52
50
36
24
20
18
18
17
17
14
14
13
13
12
12
12
10
9
7
6
6
6
6
5
5
5
4
4
4
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
538
275
151
5
5
4
3
2
2
2
1
1
1
1
1
1
1
1
1
Host
Rabbit
484
107
51
2
2
2
1
Clonality
Polyclonal
Conjugate
This VEGFR2 antibody is un-conjugated
38
25
22
16
14
12
9
7
5
4
3
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
403
231
205
126
105
79
31
27
23
22
17
16
8
6
6
5
5
2
2
1
1
1
1
1
Supplier
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Available images

Catalog No. ABIN253982
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008912129 ABIN284422 FACS IP WB Rat IgG2a Log in to see 89B3A5
0.0008912129 ABIN2657390 BR FACS IP IHC Alexa Fluor 488 Rat IgG2a kappa Log in to see Avas12 7
0.0008912129 ABIN345527 IP Rat IgG2a Log in to see 89B3A5
0.0008912129 ABIN449650 IP ELISA WB Rat IgG1 Log in to see 3C8
0.0008912129 ABIN2657391 BR FACS IP IHC Alexa Fluor 488 Rat IgG2a kappa Log in to see Avas12 7
0.0008912129 ABIN2141992 FACS IP Rat IgG2a kappa Log in to see Avas12a1 2
0.0008912129 ABIN355027 IP WB Rabbit C-Term Log in to see Polyclonal
0.0008912129 ABIN1740654 IP WB Rabbit C-Term Log in to see Polyclonal
0.0008912129 ABIN2384593 IP WB Rabbit AA 1158-1365 Log in to see Polyclonal

Top referenced anti-VEGFR2 antibody for Immunoprecipitation

Similar anti-VEGFR2 Antibodies

Application / Reactivity Mouse (Murine)
Biochemical Assay (BCA) 1 Antibodies
Blocking Reagent (BR) 2 Antibodies
ELISA 71 Antibodies
ELISA (Detection) 6 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 39 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 10 Antibodies
Immunofluorescence (IF) 55 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 103 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 43 Antibodies
Immunoprecipitation (IP) 10 Antibodies
Intracellular Flow Cytometry (ICFC) 5 Antibodies

General

Antigen Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) Antibodies
Epitope C-Term, AA 1158-1365
(79), (52), (50), (36), (24), (20), (18), (18), (17), (17), (14), (14), (13), (13), (12), (12), (12), (10), (9), (7), (6), (6), (6), (6), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(538), (275), (151), (5), (5), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(484), (107), (51), (2), (2), (2), (1)
Clonality
Polyclonal
Conjugate This VEGFR2 antibody is un-conjugated
(38), (25), (22), (16), (14), (12), (9), (7), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(403), (231), (205), (126), (105), (79), (31), (27), (23), (22), (17), (16), (8), (6), (6), (5), (5), (2), (2), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-VEGFR2 Antibody

Target Details VEGFR2 Application Details Handling References for anti-VEGFR2 Antibody (ABIN253982) Images
Purification Unpurified
Immunogen Residues corresponding to the kinase insert or C-terminus of mouse VEGF Receptor 2 (residues 1158-1365). [Swiss-Prot# P35918]

Target Details VEGFR2

Product Details anti-VEGFR2 Antibody Application Details Handling References for anti-VEGFR2 Antibody (ABIN253982) Images back to top
Antigen
Alternative Name CD309 / VEGFR-2 / Flk-1 (KDR Antibody Abstract)
Background Gene Symbol: KDR
Molecular Weight Theoretical MW: 150 kDa
UniProt P35918
Pathways RTK Signaling

Application Details

Product Details anti-VEGFR2 Antibody Target Details VEGFR2 Handling References for anti-VEGFR2 Antibody (ABIN253982) Images back to top
Application Notes Western Blot 1:2000-1:10000, Immunohistochemistry 1:10-1:500, Immunoprecipitation 2-5 μL, Immunohistochemistry-Paraffin 1:10-1:500
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for VEGF Receptor 2 Antibody Western Blot Protocol
1. Perform SDS-PAGE (3-8 %) on samples to be analyzed, loading 20 µg of total transfected protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk in TBS with 0.5 % BSA for 1 hour.
. Dilute the rabbit anti-VEGFR2 primary antibody in blocking buffer and incubate 1.5 hours at room temperature.
. Wash the membrane in water for 5 minutes and apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) and incubate 1 hour at room temperature.
. Wash the blot in TBS containing 0.05-0.1 % Tween-20 for 10-20 minutes.
. Wash the blot in type I water for an additional 10-20 minutes (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturer's instructions (Amersham's ECL is the standard reagent used).Note: Tween-20 can be added to the blocking buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Restrictions For Research Use only

Handling

Product Details anti-VEGFR2 Antibody Target Details VEGFR2 Application Details References for anti-VEGFR2 Antibody (ABIN253982) Images back to top
Format Liquid
Buffer Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-VEGFR2 Antibody (ABIN253982)

Product Details anti-VEGFR2 Antibody Target Details VEGFR2 Application Details Handling Images back to top
Product cited in:

Meyer, Singh, Rahimi: "The carboxyl terminus controls ligand-dependent activation of VEGFR-2 and its signaling." in: The Journal of biological chemistry, Vol. 279, Issue 1, pp. 735-42, 2003

Rahimi, Dayanir, Lashkari: "Receptor chimeras indicate that the vascular endothelial growth factor receptor-1 (VEGFR-1) modulates mitogenic activity of VEGFR-2 in endothelial cells." in: The Journal of biological chemistry, Vol. 275, Issue 22, pp. 16986-92, 2000

Images

Product Details anti-VEGFR2 Antibody Target Details VEGFR2 Application Details Handling References for anti-VEGFR2 Antibody (ABIN253982) back to top
Supplier Images
Western Blotting (WB) image for anti-VEGFR2 antibody (Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase)) (C-Term) (ABIN253982) anti-Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) (C-Term), (AA 1158-1365) antibody